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AB268827

Recombinant human OTUB2 protein (Active)

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Recombinant human OTUB2 protein (Active) is a Human Full Length protein, in the 1 to 234 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

C14orf137, OTB2, OTU2, OTUB2, Ubiquitin thioesterase OTUB2, Deubiquitinating enzyme OTUB2, OTU domain-containing ubiquitin aldehyde-binding protein 2, Otubain-2, Ubiquitin-specific-processing protease OTUB2

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Functional Studies - Recombinant human OTUB2 protein (Active) (AB268827)
  • FuncS

Supplier Data

Functional Studies - Recombinant human OTUB2 protein (Active) (AB268827)

The specific activity was determined to be ≥ 22 nmol/min/mg in a DUB assay using recombinant human ubiquitin-based proluciferin substrate.

SDS-PAGE - Recombinant human OTUB2 protein (Active) (AB268827)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human OTUB2 protein (Active) (AB268827)

SDS-PAGE analysis of ab268827.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity was determined to be ≥ 22 nmol/min/mg in a DUB assay using recombinant human ubiquitin-based proluciferin substrate.

Accession

Q96DC9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MSETSFNLISEKCDILSILRDHPENRIYRRKIEELSKRFTAIRKTKGDGNCFYRALGYSYLESLLGKSREIFKFKERVLQTPNDLLAAGFEEHKFRNFFNAFYSVVELVEKDGSVSSLLKVFNDQSASDHIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMATECDHIQITALSQALSIALQVEYVDEMDTALNHHVFPEAATPSVYLLYKTSHYNILYAADKH","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":234,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96DC9","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OTUB2 also known as OTU domain-containing ubiquitin aldehyde-binding protein 2 is an enzyme that acts mechanically as a deubiquitinating enzyme. It has a molecular mass of approximately 31 kDa. OTUB2 regulates ubiquitin signaling by cleaving ubiquitin from ubiquitinated proteins. This protein is expressed in various tissues with high expression in the liver and heart. It plays a role in modulating protein degradation and cellular signaling pathways.
Biological function summary

This protein influences cellular processes by interacting with ubiquitin-conjugating enzymes. OTUB2 often joins complexes that include ubiquitin-proteasome machinery components. Through these interactions it controls protein stability and degradation impacting cell cycle progression and DNA damage response. OTUB2's activity is essential for maintaining protein homeostasis which affects how cells respond to stress and changes in their environment.

Pathways

OTUB2 plays roles in the ubiquitin-proteasome and DNA repair pathways. In these pathways its interaction with other proteins like UBC13 and RAD51 is important for its function. These interactions help repair DNA damage and modulate the immune response. By regulating the stability of key signaling proteins OTUB2 ensures proper pathway function and cellular homeostasis.

OTUB2 connects to cancer and neurodegenerative diseases. In cancer its regulatory function on protein degradation affects tumor progression and resistance to therapy. With neurodegenerative diseases altered OTUB2 activity can disrupt protein homeostasis leading to accumulation of misfolded proteins. Interactions with proteins such as p53 in cancer and tau in neurodegenerative disorders link OTUB2 to disease mechanisms influencing therapeutic approaches and drug development strategies.
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Specifications

Form

Liquid

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General info

Function

Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.

Sequence similarities

Belongs to the peptidase C65 family.

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Product protocols

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Target data

Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.
See full target information OTUB2
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