Recombinant Human OTUD6B protein (His tag) is a Human Full Length protein, in the 1 to 293 aa range, expressed in Escherichia coli, with >70% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M E A V L T E E L D E E E Q L L R R H R K E K K E L Q A K I Q G M K N A V P K N D K K R R K Q L T E D V A K L E K E M E Q K H R E E L E Q L K L T T K E N K I D S V A V N I S N L V L E N Q P P R I S K A Q K R R E K K A A L E K E R E E R I A E A E I E N L T G A R H M E S E K L A Q I L A A R Q L E I K Q I P S D G H C M Y K A I E D Q L K E K D C A L T V V A L R S Q T A E Y M Q S H V E D F L P F L T N P N T G D M Y T P E E F Q K Y C E D I V N T A A W G G Q L E L R A L S H I L Q T P I E I I Q A D S P P I I V G E E Y S K K P L I L V Y M R H A Y G L G E H Y N S V T R L V N I V T E N C S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Isoform 1. Deubiquitinating enzyme that may play a role in the ubiquitin-dependent regulation of protein synthesis, downstream of mTORC1 (PubMed:21267069, PubMed:27864334). May associate with the protein synthesis initiation complex and modify its ubiquitination to repress translation (PubMed:27864334). May also repress DNA synthesis and modify different cellular targets thereby regulating cell growth and proliferation (PubMed:27864334). May also play a role in proteasome assembly and function (PubMed:28343629). Isoform 2. Stimulates protein synthesis. Influences the expression of CCND1/cyclin D1 by promoting its translation and regulates MYC/c-Myc protein stability.
Alternative names
DUBA5, CGI-77, OTUD6B, Deubiquitinase OTUD6B, DUBA-5, OTU domain-containing protein 6B
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Recombinant Human OTUD6B protein (His tag) is a Human Full Length protein, in the 1 to 293 aa range, expressed in Escherichia coli, with >70% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q8N6M0-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence
- M E A V L T E E L D E E E Q L L R R H R K E K K E L Q A K I Q G M K N A V P K N D K K R R K Q L T E D V A K L E K E M E Q K H R E E L E Q L K L T T K E N K I D S V A V N I S N L V L E N Q P P R I S K A Q K R R E K K A A L E K E R E E R I A E A E I E N L T G A R H M E S E K L A Q I L A A R Q L E I K Q I P S D G H C M Y K A I E D Q L K E K D C A L T V V A L R S Q T A E Y M Q S H V E D F L P F L T N P N T G D M Y T P E E F Q K Y C E D I V N T A A W G G Q L E L R A L S H I L Q T P I E I I Q A D S P P I I V G E E Y S K K P L I L V Y M R H A Y G L G E H Y N S V T R L V N I V T E N C S
- Accession
- Q8N6M0
- Protein length
- Full Length
- Amino acids
- 1 to 293
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Isoform 1
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
OTUD6B also known as OTU domain-containing protein 6B functions as a deubiquitinating enzyme. It specifically targets ubiquitin chains on substrate proteins for removal which regulates protein stability and activity. The molecular mass of OTUD6B is approximately 37 kDa. Researchers found its expression in a variety of tissues with higher levels in lung and placenta tissues. This suggests its importance in those areas but it also appears present in several other tissues throughout the body.
- Biological function summary
OTUD6B plays a significant role in cellular homeostasis by modulating protein turnover. Its enzymatic activity affects key regulatory proteins influencing cell cycle and signaling pathways. It does not seem to act within a larger protein complex but interacts individually with specific targets to exert its effects. Its deubiquitinating function impacts various cellular activities ensuring proper protein function and cell response.
- Pathways
OTUD6B integrates into ubiquitin-proteasome pathways and impacts the regulation of protein degradation and turnover. It closely associates with cellular processes like cell cycle progression and apoptosis. OTUD6B interacts with proteins such as p53 and NF-kB playing a part in their stabilization and activity regulation. These interactions show its role in important signaling pathways that influence cell fate and stress response.
- Associated diseases and disorders
Alterations in OTUD6B function connect to cancer and neurodegenerative diseases. Dysregulation of its deubiquitinating activity can contribute to tumorigenesis through inappropriate protein stabilization affecting tumor suppressors or oncogenes. In neurodegenerative conditions abnormalities in protein degradation pathways can arise where OTUD6B's function becomes critical. It is connected with proteins like p53 and tau which are implicated in cancer progression and Alzheimer's disease respectively. Understanding OTUD6B's role could offer insight into new therapeutic approaches for these disorders.
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1 product image
SDS-PAGE - Recombinant Human OTUD6B protein (His tag) (ab268828)
SDS-PAGE analysis of ab268828.
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