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AB268828

Recombinant Human OTUD6B protein (His tag)

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Recombinant Human OTUD6B protein (His tag) is a Human Full Length protein, in the 1 to 293 aa range, expressed in Escherichia coli, with >70%, suitable for SDS-PAGE.

View Alternative Names

DUBA5, CGI-77, OTUD6B, Deubiquitinase OTUD6B, DUBA-5, OTU domain-containing protein 6B

1 Images
SDS-PAGE - Recombinant Human OTUD6B protein (His tag) (AB268828)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human OTUD6B protein (His tag) (AB268828)

SDS-PAGE analysis of ab268828.

Key facts

Purity

>70% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q8N6M0

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MEAVLTEELDEEEQLLRRHRKEKKELQAKIQGMKNAVPKNDKKRRKQLTEDVAKLEKEMEQKHREELEQLKLTTKENKIDSVAVNISNLVLENQPPRISKAQKRREKKAALEKEREERIAEAEIENLTGARHMESEKLAQILAARQLEIKQIPSDGHCMYKAIEDQLKEKDCALTVVALRSQTAEYMQSHVEDFLPFLTNPNTGDMYTPEEFQKYCEDIVNTAAWGGQLELRALSHILQTPIEIIQADSPPIIVGEEYSKKPLILVYMRHAYGLGEHYNSVTRLVNIVTENCS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":293,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8N6M0","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OTUD6B also known as OTU domain-containing protein 6B functions as a deubiquitinating enzyme. It specifically targets ubiquitin chains on substrate proteins for removal which regulates protein stability and activity. The molecular mass of OTUD6B is approximately 37 kDa. Researchers found its expression in a variety of tissues with higher levels in lung and placenta tissues. This suggests its importance in those areas but it also appears present in several other tissues throughout the body.
Biological function summary

OTUD6B plays a significant role in cellular homeostasis by modulating protein turnover. Its enzymatic activity affects key regulatory proteins influencing cell cycle and signaling pathways. It does not seem to act within a larger protein complex but interacts individually with specific targets to exert its effects. Its deubiquitinating function impacts various cellular activities ensuring proper protein function and cell response.

Pathways

OTUD6B integrates into ubiquitin-proteasome pathways and impacts the regulation of protein degradation and turnover. It closely associates with cellular processes like cell cycle progression and apoptosis. OTUD6B interacts with proteins such as p53 and NF-kB playing a part in their stabilization and activity regulation. These interactions show its role in important signaling pathways that influence cell fate and stress response.

Alterations in OTUD6B function connect to cancer and neurodegenerative diseases. Dysregulation of its deubiquitinating activity can contribute to tumorigenesis through inappropriate protein stabilization affecting tumor suppressors or oncogenes. In neurodegenerative conditions abnormalities in protein degradation pathways can arise where OTUD6B's function becomes critical. It is connected with proteins like p53 and tau which are implicated in cancer progression and Alzheimer's disease respectively. Understanding OTUD6B's role could offer insight into new therapeutic approaches for these disorders.
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Specifications

Form

Liquid

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General info

Function

Isoform 1. Deubiquitinating enzyme that may play a role in the ubiquitin-dependent regulation of protein synthesis, downstream of mTORC1 (PubMed : 21267069, PubMed : 27864334). May associate with the protein synthesis initiation complex and modify its ubiquitination to repress translation (PubMed : 27864334). May also repress DNA synthesis and modify different cellular targets thereby regulating cell growth and proliferation (PubMed : 27864334). May also play a role in proteasome assembly and function (PubMed : 28343629).. Isoform 2. Stimulates protein synthesis. Influences the expression of CCND1/cyclin D1 by promoting its translation and regulates MYC/c-Myc protein stability.

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Product protocols

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Target data

Isoform 1. Deubiquitinating enzyme that may play a role in the ubiquitin-dependent regulation of protein synthesis, downstream of mTORC1 (PubMed : 21267069, PubMed : 27864334). May associate with the protein synthesis initiation complex and modify its ubiquitination to repress translation (PubMed : 27864334). May also repress DNA synthesis and modify different cellular targets thereby regulating cell growth and proliferation (PubMed : 27864334). May also play a role in proteasome assembly and function (PubMed : 28343629).. Isoform 2. Stimulates protein synthesis. Influences the expression of CCND1/cyclin D1 by promoting its translation and regulates MYC/c-Myc protein stability.
See full target information OTUD6B
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