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AB268829

Recombinant Human OTULIN protein (His tag)

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Recombinant Human OTULIN protein (His tag) is a Human Full Length protein, in the 1 to 352 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

FAM105B, OTULIN, Ubiquitin thioesterase otulin, Deubiquitinating enzyme otulin, OTU domain-containing deubiquitinase with linear linkage specificity, Ubiquitin thioesterase Gumby

1 Images
SDS-PAGE - Recombinant Human OTULIN protein (His tag) (AB268829)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human OTULIN protein (His tag) (AB268829)

SDS-PAGE analysis of ab268829.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q96BN8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MSRGTMPQPEAWPGASCAETPAREAAATARDGGKAAASGQPRPEMQCPAEHEEDMYRAADEIEKEKELLIHERGASEPRLSVAPEMDIMDYCKKEWRGNTQKATCMKMGYEEVSQKFTSIRRVRGDNYCALRATLFQAMSQAVGLPPWLQDPELMLLPEKLISKYNWIKQWKLGLKFDGKNEDLVDKIKESLTLLRKKWAGLAEMRTAEARQIACDELFTNEAEEYSLYEAVKFLMLNRAIELYNDKEKGKEVPFFSVLLFARDTSNDPGQLLRNHLNQVGHTGGLEQVEMFLLAYAVRHTIQVYRLSKYNTEEFITVYPTDPPKDWPVVTLIAEDDRHYNIPVRVCEETSL","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":352,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96BN8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OTULIN also known as OTU deubiquitinase with linear linkage specificity is a deubiquitinating enzyme that cleaves linear polyubiquitin chains from proteins. It exhibits a mass of approximately 42 kDa. OTULIN expresses widely in tissues but has higher expression in immune cells. Its mechanical action involves hydrolyzing Met1-linked ubiquitin chains which are unique among polyubiquitin linkages for being linear rather than branched.
Biological function summary

OTULIN regulates inflammation and immune signaling. It often functions as a part of the LUBAC (linear ubiquitin chain assembly complex) ensuring homeostasis by preventing excessive activation of immune responses. OTULIN’s activity modulates NF-kB signaling an important pathway for immune and inflammatory responses. The enzyme maintains this balance to prevent unwanted inflammation promoting cellular health.

Pathways

OTULIN influences both the NF-kB pathway and the Wnt signaling pathway. In the NF-kB pathway it interacts closely with proteins such as NEMO (NF-kB essential modulator) and inhibits prolonged activation by removing linear ubiquitin chains. Likewise in the Wnt pathway OTULIN's role involves the regulation of signaling having indirect effects on cell proliferation and differentiation processes. Its ability to modulate these pathways highlights its integral role in maintaining immune function and development.

OTULIN is linked to autoimmune and inflammatory conditions such as OTULIN-related autoinflammatory syndrome (ORAS) and rheumatoid arthritis. Mutations in the OTULIN gene can lead to dysregulation in inflammation indicating its significance in immune system-related diseases. Additionally through its interaction with proteins such as ubiquitin disruptions in OTULIN function may contribute to improper immune responses enhancing disease susceptibility.
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Specifications

Form

Liquid

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General info

Function

Deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response (PubMed : 23708998, PubMed : 23746843, PubMed : 23806334, PubMed : 23827681, PubMed : 24726323, PubMed : 24726327, PubMed : 26997266, PubMed : 27523608, PubMed : 27559085, PubMed : 28919039, PubMed : 35170849, PubMed : 35587511). Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex (PubMed : 23708998). Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex (PubMed : 23746843, PubMed : 23806334). OTULIN function is mainly restricted to homeostasis of the LUBAC complex : acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex (PubMed : 26670046). Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis (PubMed : 27523608). In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation (PubMed : 27523608). Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent pro-inflammatory signaling (PubMed : 23806334).

Sequence similarities

Belongs to the peptidase C65 family. Otulin subfamily.

Post-translational modifications

Ubiquitinated.. Acetylated.. Phosphorylated (PubMed:23746843, PubMed:24726323, PubMed:24726327). Phosphorylation at Tyr-56 prevents interaction with RNF31; dephosphorylation promotes interaction with RNF31 and the LUBAC complex (PubMed:24726323, PubMed:24726327).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response (PubMed : 23708998, PubMed : 23746843, PubMed : 23806334, PubMed : 23827681, PubMed : 24726323, PubMed : 24726327, PubMed : 26997266, PubMed : 27523608, PubMed : 27559085, PubMed : 28919039, PubMed : 35170849, PubMed : 35587511). Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex (PubMed : 23708998). Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex (PubMed : 23746843, PubMed : 23806334). OTULIN function is mainly restricted to homeostasis of the LUBAC complex : acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex (PubMed : 26670046). Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis (PubMed : 27523608). In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation (PubMed : 27523608). Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent pro-inflammatory signaling (PubMed : 23806334).
See full target information OTULIN
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