Recombinant Human p21-ARC/ARPC3 protein is a Human Full Length protein, in the 1 to 178 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M P A Y H S S L M D P D T K L I G N M A L L P I R S Q F K G P A P R E T K D T D I V D E A I Y Y F K A N V F F K N Y E I K N E A D R T L I Y I T L Y I S E C L K K L Q K C N S K S Q G E K E M Y T L G I T N F P I P G E P G F P L N A I Y A K P A N K Q E D E V M R A Y L Q Q L R Q E T G L R L C E K V F D P Q N D K P S K W W T C F V K R Q F M N K S L S G P G Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).
Alternative names
ARC21, ARPC3, Actin-related protein 2/3 complex subunit 3, Arp2/3 complex 21 kDa subunit, p21-ARC
Recommended products
Recombinant Human p21-ARC/ARPC3 protein is a Human Full Length protein, in the 1 to 178 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- O15145-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M P A Y H S S L M D P D T K L I G N M A L L P I R S Q F K G P A P R E T K D T D I V D E A I Y Y F K A N V F F K N Y E I K N E A D R T L I Y I T L Y I S E C L K K L Q K C N S K S Q G E K E M Y T L G I T N F P I P G E P G F P L N A I Y A K P A N K Q E D E V M R A Y L Q Q L R Q E T G L R L C E K V F D P Q N D K P S K W W T C F V K R Q F M N K S L S G P G Q
- Accession
- O15145
- Protein length
- Full Length
- Predicted molecular weight
- 22.9 kDa
- Amino acids
- 1 to 178
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab140715 is purified using conventional chromatography techniques.
General info
- Function
Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).
- Sequence similarities
Belongs to the ARPC3 family.
- Subcellular localisation
- Cytoskeleton, Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as p21-ARC
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The p21-ARC also known as ARPC3 is a subunit of the actin-related protein 2/3 complex (Arp2/3 complex). This protein plays a critical role in the nucleation of new actin filaments promoting the formation of branched actin networks. ARPC3 has a molecular mass of approximately 21 kDa and its expression can be found in a variety of tissues including the heart and kidney. The protein operates in the cytoplasm where it associates with other proteins in the Arp2/3 complex to mediate its function.
- Biological function summary
The p21-ARC/ARPC3 is an essential component of the highly conserved Arp2/3 complex involved in actin filament assembly and organization. This complex binds to the sides of pre-existing actin filaments and nucleates new ones creating branching and forming web-like structures needed for various cellular processes. The Arp2/3 complex including ARPC3 is involved in cell movement endocytosis and intracellular trafficking. This protein's activity enhances the dynamic restructuring of the actin cytoskeleton necessary for these functions.
- Pathways
ARPC3 influences pathways related to cell motility and cytoskeletal reorganization. It participates in the Wiskott-Aldrich Syndrome Protein (WASP) pathway where it acts alongside proteins such as WASP and the WAVE complex to regulate actin polymerization. The process converts signals from the cell surface into mechanical force driving the extension of pseudopodia and lamellipodia. Another important pathway involves the Rho family GTPases where ARPC3 links to actin dynamics cell polarity and the orchestration of actin assembly in response to signaling events within the cell.
- Associated diseases and disorders
Abnormalities in ARPC3 function or expression can influence neurological and immunological conditions. For instance dysregulation of the Arp2/3 complex can enhance susceptibility to diseases like Wiskott-Aldrich syndrome which is tied to impaired signaling and cytoskeletal rearrangement. ARPC3 interacts with proteins related to immune response such as WASP making it significant in the context of immune deficiency disorders. Furthermore mutations affecting actin cytoskeleton dynamics can lead to neurodegenerative diseases highlighting the important impact of ARPC3 and associated Arp2/3 complex proteins in maintaining cellular function.
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1 product image
SDS-PAGE - Recombinant Human p21-ARC/ARPC3 protein (ab140715)
15% SDS-PAGE analysis of ab140715 (3μg).
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