Recombinant Human p21 protein is a Human Full Length protein, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, WB, FuncS.
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- Radiation oncology (London, England) 10:1522015Increased SHP-1 expression results in radioresistance, inhibition of cellular senescence, and cell cycle redistribution in nasopharyngeal carcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiyi Sun et. al.PubMed 26215037
- Oncoimmunology 3:e9522022014Anti-p21 autoantibodies detected in colorectal cancer patients: A proof of concept study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFaraz Bishehsari,Johannes S Gach,Naomi Akagi,Molly K Webber,Jessica Bauer,Barbara H JungPubMed 25960931
Key facts
- Purity
>80% SDS-PAGE
- Expression system
Escherichia coli
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, WB, FuncS
- Biologically active
No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab56278 can be used as a WB positive control in conjunction with ab18209. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes Kinase Assay. |
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Target data
Function
May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739). Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest (PubMed:9106657).
Alternative names
Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, p21, MDA-6, CDKN1A, MDA6, CAP20, CDKN1, CIP1, PIC1, SDI1, WAF1
Recommended products
Recombinant Human p21 protein is a Human Full Length protein, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, WB, FuncS.
Alternative names
Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, p21, MDA-6, CDKN1A, MDA6, CAP20, CDKN1, CIP1, PIC1, SDI1, WAF1
Key facts
- Purity
>80% SDS-PAGE
- Expression system
Escherichia coli
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, WB, FuncS
- Biologically active
No
- Accession
- P38936-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF
Sequence info
Amino acid sequence
- Accession
- Protein length
Full Length
- Nature
Recombinant
- Tags
GST tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739). Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest (PubMed:9106657).
- Sequence similarities
Belongs to the CDI family.
- Post-translational modifications
Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell proliferation. Phosphorylation of Thr-145 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. UV radiation-induced phosphorylation at Thr-80 by LKB1 and at Ser-146 by NUAK1 leads to its degradation.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The p21 protein also known as CDKN1A is an important regulator of cell cycle progression. It acts as a cyclin-dependent kinase inhibitor where it binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes. The molecular weight of p21 is approximately 21 kDa. Cellular expression of p21 is diverse; it occurs in various tissues reflecting its broad role in maintaining cell cycle control. This protein's expression level is often assessed using techniques such as p21 western blotting p21 ELISA and p21 IHC staining.
- Biological function summary
P21 plays a critical role in cell cycle regulation and DNA damage response. It associates with the p53 tumor suppressor protein forming an essential part of the p53 signaling pathway under stress conditions. p21 arrests cells in G1 phase allowing DNA repair or leading to cellular senescence. Through these mechanisms p21 integrates signals from stress and damage emphasizing its importance as a mediator in cellular checkpoint control.
- Pathways
P21 is closely involved in the p53 and the Transforming Growth Factor-beta (TGF-β) signaling pathways. This integration helps in transmitting growth-inhibitory signals. p21 interacts with proteins such as p53 and cyclins ensuring precise regulation of the cell cycle and growth arrest when necessary. Triggered by p53 activation p21 contributes to preventing undisturbed cell proliferation acting as a safeguard against tumor formation.
- Associated diseases and disorders
P21's deregulation associates with cancer and age-related diseases. Its impairment is a common feature in many cancers often linked with defects in the p53 pathway. Reduced p21 expression can contribute to unchecked cell division and tumor progression. Moreover in age-related diseases p21's role in promoting cellular senescence connects it with degenerative conditions. The intricate relationship between p21 and proteins such as p53 and cyclins highlights its potential as a therapeutic target for these disorders.
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2 product images
SDS-PAGE - Recombinant Human p21 protein (ab56278)
SDS-PAGE analysis of ab56278 with molecular weight markers. Approximate molecular weight 46kDa.
Sandwich ELISA - Recombinant Human p21 protein (ab56278)
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