Recombinant Human p27 KIP 1 protein (Tagged) is a Human Full Length protein, in the 1 to 198 aa range, expressed in Escherichia coli, with >70% purity and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Purity
- >70%
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M S N V R V S N G S P S L E R M D A R Q A E H P K P S A C R N L F G P V D H E E L T R D L E K H C R D M E E A S Q R K W N F D F Q N H K P L E G K Y E W Q E V E K G S L P E F Y Y R P P R P P K G A C K V P A Q E S Q D V S G S R P A A P L I G A P A N S E D T H L V D P K T D P S D S Q T G L A E Q C A G I R K R P A T D D S S T Q N K R A N R T E E N V S D G S P N A G S V E Q T P K K P G L R R R Q T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab56279 can be used as a WB positive control in conjunction with ab7961. |
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Target data
Function
Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Alternative names
KIP1, p27, CDKN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1
Recommended products
Recombinant Human p27 KIP 1 protein (Tagged) is a Human Full Length protein, in the 1 to 198 aa range, expressed in Escherichia coli, with >70% purity and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Purity
- >70%
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P46527-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF
Sequence info
Amino acid sequence
- M S N V R V S N G S P S L E R M D A R Q A E H P K P S A C R N L F G P V D H E E L T R D L E K H C R D M E E A S Q R K W N F D F Q N H K P L E G K Y E W Q E V E K G S L P E F Y Y R P P R P P K G A C K V P A Q E S Q D V S G S R P A A P L I G A P A N S E D T H L V D P K T D P S D S Q T G L A E Q C A G I R K R P A T D D S S T Q N K R A N R T E E N V S D G S P N A G S V E Q T P K K P G L R R R Q T
- Accession
- P46527
- Protein length
- Full Length
- Predicted molecular weight
- 52 kDa
- Amino acids
- 1 to 198
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purified by affinity chromatography
General info
- Function
Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
- Sequence similarities
Belongs to the CDI family.
- Post-translational modifications
Phosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK1 and CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF.
- Subcellular localisation
- Nucleus, Endosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein known as p27 KIP1 also referred to as p27 CDKN1B or KIP1 is a member of the kinase inhibitory protein family. It plays an important role in cell cycle regulation by inhibiting cyclin-dependent kinases (CDKs). p27 KIP1 weighs approximately 27 kDa and can be found in various tissues where it regulates cell proliferation. The protein functions as a suppressor by binding to cyclin-CDK complexes preventing the transition from G1 phase to S phase in the cell division cycle.
- Biological function summary
The function of p27 KIP1 involves its role in controlling cell growth and division. It achieves this by becoming a part of larger protein complexes involving CDKs and cyclins. By directly interacting with these complexes p27 KIP1 modulates cell cycle progression therefore acting as a brake on cellular proliferation. The protein is important in maintaining proper cell cycle checkpoints and preventing uncontrolled cell growth which is essential for normal cellular functioning.
- Pathways
The involvement of p27 KIP1 centers on cell cycle regulation and signaling pathways such as the PI3K/AKT pathway. Its interaction with CDKs and cyclins situates it within the core mechanisms that determine cell division timing. p27 KIP1 operates alongside other proteins like cyclin D and CDK4/6 fitting into the regulatory intricacies of these pathways. Proper functioning of these pathways ensures cellular homeostasis and prevents the development of oncogenic processes.
- Associated diseases and disorders
Dysfunction of p27 KIP1 has links to cancer and neurodegenerative diseases. Low levels or mutations can lead to uncontrolled cell proliferation contributing to the development and progression of cancers such as breast cancer. p27 KIP1's role in neurodegenerative diseases involves its regulation of neuronal cell cycle re-entry with abnormalities potentially exacerbating conditions like Alzheimer's disease. In these contexts its interaction with proteins such as cyclin E and CDK2 becomes particularly relevant in understanding disease mechanisms.
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3 product images
Sandwich ELISA - Recombinant Human p27 KIP 1 protein (Tagged) (ab56279)
SDS-PAGE - Recombinant Human p27 KIP 1 protein (Tagged) (ab56279)
SDS-PAGE analysis of ab56279 with molecular weight markers. Approximate molecular weight 52kDa.
Western blot - Recombinant Human p27 KIP 1 protein (Tagged) (ab56279)
ab7961 recognizes the tagged recombinant p27 KIP 1 protein (ab56279) which has an expected molecular weight of 52 kDa.
All lanes: Anti-p27 KIP 1 antibody (ab7961) at 1 µg/mL
All lanes: Western blot - Recombinant Human p27 KIP 1 protein (Tagged) (ab56279) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10s
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