Recombinant Human p35 protein
Be the first to review this product! Submit a review
|
(0 Publication)
Recombinant Human p35 protein is a Human Full Length protein, in the 1 to 307 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, WB.
View Alternative Names
CDK5R, NCK5A, CDK5R1, Cyclin-dependent kinase 5 activator 1, CDK5 activator 1, Cyclin-dependent kinase 5 regulatory subunit 1, TPKII regulatory subunit
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human p35 protein (AB119724)
SDS Page analysis of ab119724.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
P35 is essential for the regulation of many aspects of neural development including cell migration and axonal growth. It forms a complex with Cdk5 which then phosphorylates various substrates that are critical for these processes. The p35/Cdk5 complex influences the cytoskeletal dynamics needed for neuronal positioning and dendritic spine formation and it is significant for learning and memory.
Pathways
P35 serves in the neuronal signaling and cell cycle pathways. The Cdk5/p35 complex interfaces with pathways such as those involving the neurofilament proteins important for maintaining neuronal structure and function. In the cell cycle though atypical Cdk5/p35 can affect pathways related to cell cycle arrest highlighting its diverse functional connectivity.
Specifications
Form
Liquid
Additional notes
The purity was determined to be 70% by densitometry. Affinity purified.
General info
Function
p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein : phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-BMAL1 heterodimer in association with altered stability and subcellular distribution.
Sequence similarities
Belongs to the cyclin-dependent kinase 5 activator family.
Post-translational modifications
The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo (By similarity).. Myristoylated. A proper myristoylation signal is essential for the proper distribution of p35.. Ubiquitinated, leading to its degradation: degradation of p35 by proteasome results in down-regulation of CDK5 activity (PubMed:12393264). During this process, CDK5 phosphorylates p35 and induces its ubiquitination and subsequent degradation (PubMed:12393264). Ubiquitinated by the CRL2(FEM1B) complex, which recognizes the -Gly-Leu-Asp-Arg C-degron at the C-terminus, leading to its degradation (PubMed:33398168, PubMed:33398170).. Phosphorylation at Ser-8 and Thr-138 by CDK5 prevents calpain-mediated proteolysis.
Subcellular localisation
Nucleus
Target data
Complementary Products
![Western blot - Anti-CDK5 antibody [EP715Y] (AB40773)](https://content.abcam.com/products/images/cdk5-antibody-ep715y-ab40773--western-blot-img6610.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com