Recombinant human p38 delta/MAPK13 protein is a Human Full Length protein, in the 1 to 365 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
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Key facts
- Purity
- >90% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK13 is one of the less studied p38 MAPK isoforms. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K. Involved in cytoskeletal remodeling through phosphorylation of MAPT and STMN1. Mediates UV irradiation induced up-regulation of the gene expression of CXCL14. Plays an important role in the regulation of epidermal keratinocyte differentiation, apoptosis and skin tumor development. Phosphorylates the transcriptional activator MYB in response to stress which leads to rapid MYB degradation via a proteasome-dependent pathway. MAPK13 also phosphorylates and down-regulates PRKD1 during regulation of insulin secretion in pancreatic beta cells.
Alternative names
PRKM13, SAPK4, MAPK13, Mitogen-activated protein kinase 13, MAP kinase 13, MAPK 13, Mitogen-activated protein kinase p38 delta, Stress-activated protein kinase 4, MAP kinase p38 delta
Recommended products
Recombinant human p38 delta/MAPK13 protein is a Human Full Length protein, in the 1 to 365 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >90% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab125643 was determined to be 120 nmol/min/mg as per activity assay protocol and equivalent to 151 nmol/min/mg as per radiometric assay
- Accession
- O15264-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- Accession
- O15264
- Protein length
- Full Length
- Predicted molecular weight
- 71 kDa
- Amino acids
- 1 to 365
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Purity was determined to be >90% by densitometry. Affinity purified.
General info
- Function
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK13 is one of the less studied p38 MAPK isoforms. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K. Involved in cytoskeletal remodeling through phosphorylation of MAPT and STMN1. Mediates UV irradiation induced up-regulation of the gene expression of CXCL14. Plays an important role in the regulation of epidermal keratinocyte differentiation, apoptosis and skin tumor development. Phosphorylates the transcriptional activator MYB in response to stress which leads to rapid MYB degradation via a proteasome-dependent pathway. MAPK13 also phosphorylates and down-regulates PRKD1 during regulation of insulin secretion in pancreatic beta cells.
- Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
- Post-translational modifications
Dually phosphorylated on Thr-180 and Tyr-182 by MAP2K3/MKK3, MAP2K4/MKK4, MAP2K6/MKK6 and MAP2K7/MKK7, which activates the enzyme. Dephosphorylated by dual specificity phosphatase DUSP1.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
P38 delta also known as MAPK13 is a member of the mitogen-activated protein kinase (MAPK) family. It has a molecular weight of about 43 kDa. This kinase is involved in transmitting signals from the cell surface to the DNA in the cell nucleus affecting gene expression. You can find MAPK13 expression in various tissues such as skin lung and pancreas. It plays a role in cellular responses to stress and inflammation helping to regulate diverse cellular processes like gene expression differentiation and apoptosis.
- Biological function summary
The function of MAPK13 extends to its involvement in multiple cellular processes linked to stress responses. It forms part of a signaling complex that responds to extracellular stimuli. MAPK13 contributes to regulating inflammation by mediating the transcriptional activity of specific genes. Through phosphorylation it modifies other proteins and influences pathways that control cell growth and apoptosis. Cellular stress and environmental changes activate this kinase highlighting its role in helping cells adapt.
- Pathways
MAPK13 integrates into the MAPK signaling pathway contributing to the transmission and amplification of signals related to cellular stress and inflammatory responses. It interacts with other proteins like MAPK14 (p38 alpha) within these pathways emphasizing its role in mediating cellular reactions to stress. The MAPK pathway influences the activity of transcription factors impacting gene expression and cellular outcomes. MAPK13 also plays a role in the p38 MAPK pathway emphasizing its connection within these essential signaling networks.
- Associated diseases and disorders
MAPK13 relates to conditions such as inflammatory diseases and cancer. Its role in inflammation links MAPK13 to diseases like chronic obstructive pulmonary disease (COPD) where excessive inflammatory responses contribute to disease progression. It also connects to cancer progression where abnormal cell signaling leads to uncontrolled growth. In these conditions MAPK13 interacts with other proteins such as NF-kB which further influence disease outcomes by modulating inflammatory and cell survival pathways.
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4 product images
Functional Studies - Recombinant human p38 delta/MAPK13 protein (ab125643)
The specific activity of MAPK 13 (ab125643) was determined to be 128 nmol/min/mg as per activity assay protocol and was equivalent to 161 nmol/min/mg as per radiometric assay
SDS-PAGE - Recombinant human p38 delta/MAPK13 protein (ab125643)
SDS PAGE analysis of ab125643
SDS-PAGE - Recombinant human p38 delta/MAPK13 protein (ab125643)
SDS-PAGE analysis of ab125643.
Functional Studies - Recombinant human p38 delta/MAPK13 protein (ab125643)
The specific activity of ab125643 was determined to be 140 nmol/min/mg by Kinase Assay.
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