Recombinant human p38 gamma/MAPK12 protein is a Human Full Length protein, in the 1 to 367 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
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- Cell metabolism 32:643-653.e42020Microbial Imidazole Propionate Affects Responses to Metformin through p38γ-Dependent Inhibitory AMPK Phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAra Koh et. al.PubMed 32783890
Key facts
- Purity
- >90% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. Phosphorylates DLG1. Following osmotic shock, MAPK12 in the cell nucleus increases its association with nuclear DLG1, thereby causing dissociation of DLG1-SFPQ complexes. This function is independent of its catalytic activity and could affect mRNA processing and/or gene transcription to aid cell adaptation to osmolarity changes in the environment. Regulates UV-induced checkpoint signaling and repair of UV-induced DNA damage and G2 arrest after gamma-radiation exposure. MAPK12 is involved in the regulation of SLC2A1 expression and basal glucose uptake in L6 myotubes; and negatively regulates SLC2A4 expression and contraction-mediated glucose uptake in adult skeletal muscle. C-Jun (JUN) phosphorylation is stimulated by MAPK14 and inhibited by MAPK12, leading to a distinct AP-1 regulation. MAPK12 is required for the normal kinetochore localization of PLK1, prevents chromosomal instability and supports mitotic cell viability. MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration.
Alternative names
ERK6, SAPK3, MAPK12, Mitogen-activated protein kinase 12, MAP kinase 12, MAPK 12, Extracellular signal-regulated kinase 6, Mitogen-activated protein kinase p38 gamma, Stress-activated protein kinase 3, ERK-6, MAP kinase p38 gamma
Recommended products
Recombinant human p38 gamma/MAPK12 protein is a Human Full Length protein, in the 1 to 367 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >90% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab125651 was determined to be 220 nmol/min/mg.
- Accession
- P53778-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- Accession
- P53778
- Protein length
- Full Length
- Predicted molecular weight
- 71 kDa
- Amino acids
- 1 to 367
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Purity was determined to be >90% by densitometry. Affinity purified.
General info
- Function
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. Phosphorylates DLG1. Following osmotic shock, MAPK12 in the cell nucleus increases its association with nuclear DLG1, thereby causing dissociation of DLG1-SFPQ complexes. This function is independent of its catalytic activity and could affect mRNA processing and/or gene transcription to aid cell adaptation to osmolarity changes in the environment. Regulates UV-induced checkpoint signaling and repair of UV-induced DNA damage and G2 arrest after gamma-radiation exposure. MAPK12 is involved in the regulation of SLC2A1 expression and basal glucose uptake in L6 myotubes; and negatively regulates SLC2A4 expression and contraction-mediated glucose uptake in adult skeletal muscle. C-Jun (JUN) phosphorylation is stimulated by MAPK14 and inhibited by MAPK12, leading to a distinct AP-1 regulation. MAPK12 is required for the normal kinetochore localization of PLK1, prevents chromosomal instability and supports mitotic cell viability. MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration.
- Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
- Post-translational modifications
Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K3/MKK3 and MAP2K6/MKK6, which activates the enzyme.
- Subcellular localisation
- Nucleus, Mitochondrion
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The p38 gamma protein also known as MAPK12 is a serine/threonine-protein kinase belonging to the mitogen-activated protein kinase (MAPK) family. It has a molecular mass of approximately 41 kDa. This protein is heavily expressed in skeletal muscle and heart tissues although expression occurs in other tissues at lower levels. p38 gamma plays a mechanical role in response to stress signals by phosphorylating downstream targets affecting cellular responses such as proliferation and differentiation.
- Biological function summary
P38 gamma/MAPK12 influences the regulation of gene expression apoptosis and cell cycle processes. It operates within a specific MAP kinase signaling module that conveys signals from the cellular surface to the nucleus. This protein sometimes works in conjunction with other members of the MAPK family forming complexes that further fine-tune cellular responses under various conditions. Its activities help cells adapt to changes in their environment by modulating transcription factors and other important substrates.
- Pathways
The MAPK pathway and the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) pathway both involve p38 gamma/MAPK12. Within these pathways p38 gamma is closely related to proteins such as JNK1 and JNK2 which also respond to stress signals. These pathways are essential for transmitting signals that regulate cellular growth differentiation and stress responses revealing intricacies of cellular adaptation to the environment.
- Associated diseases and disorders
Aberrant activation or dysfunction of p38 gamma/MAPK12 relates to conditions like cancer and cardiac hypertrophy. In cancer p38 gamma can influence tumor progression and metastasis through its impact on cell proliferation and survival. It also interacts with other proteins like MKK3 and MKK6 which serve as upstream activators in oncogenic signaling pathways. In cardiac hypertrophy altered p38 gamma signaling affects cardiac muscle cell growth potentially leading to heart failure. Understanding these connections provides insight into the mechanisms underlying these disorders.
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2 product images
SDS-PAGE - Recombinant human p38 gamma/MAPK12 protein (ab125651)
SDS-PAGE analysis of ab125651.
Functional Studies - Recombinant human p38 gamma/MAPK12 protein (ab125651)
The specific activity of ab125651 was determined to be 220 nmol/min/mg by Kinase Assay.
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