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AB114624

Recombinant Human P4HA1 protein

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Recombinant Human P4HA1 protein is a Human Full Length protein, in the 1 to 534 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

P4HA, P4HA1, Prolyl 4-hydroxylase subunit alpha-1, 4-PH alpha-1

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SDS-PAGE - Recombinant Human P4HA1 protein (AB114624)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human P4HA1 protein (AB114624)

ab114624 analysed on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

No

Accession

P13674

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MIWYILIIGILLPQSLAHPGFFTSIGQMTDLIHTEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLVLKDMSDGFISNLTIQRQYFPNDEDQVGAAKALLRLQDTYNLDTDTISKGNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALRQLDEGEISTIDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE","proteinLength":"Full Length","predictedMolecularWeight":"84.81 kDa","actualMolecularWeight":null,"aminoAcidEnd":534,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P13674","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein P4HA1 also known as Prolyl 4-Hydroxylase Subunit Alpha 1 functions mechanically by catalyzing the hydroxylation of proline residues in collagen. This activity requires iron and ascorbate as cofactors. P4HA1 is a part of the prolyl 4-hydroxylase enzyme complex and contains a molecular mass of approximately 63 kDa. It expresses widely in various human tissues with high expression in the liver skin and vascular tissues reflecting its role in collagen synthesis.
Biological function summary

P4HA1 plays a central role in collagen biosynthesis and maturation by introducing hydroxyl groups into proline residues which stabilize the collagen triple helix. This protein functions as a critical component of the prolyl 4-hydroxylase complex which includes a beta subunit. Proper formation and stability of collagen fibers depend on P4HA1 activity impacting the structural integrity of connective tissues throughout the body.

Pathways

P4HA1's function integrates into the pathways of collagen biosynthesis and modification. It is significant in the extracellular matrix organization pathway which upholds the structural support and cellular signaling. Additionally P4HA1 interacts with other proteins like COL1A1 and PLOD1 during these processes assisting in the proper formation of extracellular matrix structures essential for tissue function and injury repair.

P4HA1 has a strong association with fibrotic diseases such as liver fibrosis and pulmonary fibrosis where excessive collagen deposition is a hallmark. Dysregulation of P4HA1 may also play a role in tumor progression in cancers by promoting extracellular matrix remodeling therefore facilitating tumor invasion and metastasis. The protein interacts with pathways involving vascular endothelial growth factor (VEGF) which drives angiogenesis and is implicated in these disease processes.
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Specifications

Form

Liquid

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General info

Function

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

Sequence similarities

Belongs to the P4HA family.

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Product protocols

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Target data

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
See full target information P4HA1
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Complementary Products

Primary Antibodies

AB59497

Anti-P4HA1 antibody

Immunocytochemistry - Anti-P4HA1 antibody (AB59497)

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Proteins & Peptides

AB115719

Recombinant Human P4HB protein

Western blot - Recombinant Human P4HB protein (AB115719)

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Primary Antibodies

AB137110

Anti-P4HB antibody [EPR9499]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-P4HB antibody [EPR9499] (AB137110)

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  • 6
Biochemicals

AB142704

2-(1,8-Naphthyridin-2-ly)phenol, STAT1 transcriptional activator

Chemical Structure - 2-(1,8-Naphthyridin-2-ly)phenol, STAT1 transcriptional activator (AB142704)

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