Recombinant Human P4HA1 protein is a Human Full Length protein, in the 1 to 534 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M I W Y I L I I G I L L P Q S L A H P G F F T S I G Q M T D L I H T E K D L V T S L K D Y I K A E E D K L E Q I K K W A E K L D R L T S T A T K D P E G F V G H P V N A F K L M K R L N T E W S E L E N L V L K D M S D G F I S N L T I Q R Q Y F P N D E D Q V G A A K A L L R L Q D T Y N L D T D T I S K G N L P G V K H K S F L T A E D C F E L G K V A Y T E A D Y Y H T E L W M E Q A L R Q L D E G E I S T I D K V S V L D Y L S Y A V Y Q Q G D L D K A L L L T K K L L E L D P E H Q R A N G N L K Y F E Y I M A K E K D V N K S A S D D Q S D Q K T T P K K K G V A V D Y L P E R Q K Y E M L C R G E G I K M T P R R Q K K L F C R Y H D G N R N P K F I L A P A K Q E D E W D K P R I I R F H D I I S D A E I E I V K D L A K P R L R R A T I S N P I T G D L E T V H Y R I S K S A W L S G Y E N P V V S R I N M R I Q D L T G L D V S T A E E L Q V A N Y G V G G Q Y E P H F D F A R K D E P D A F K E L G T G N R I A T W L F Y M S D V S A G G A T V F P E V G A S V W P K K G T A V F W Y N L F A S G E G D Y S T R H A A C P V L V G N K W V S N K W L H E R G Q E F R R P C T L S E L E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Alternative names
P4HA, P4HA1, Prolyl 4-hydroxylase subunit alpha-1, 4-PH alpha-1
Recommended products
Recombinant Human P4HA1 protein is a Human Full Length protein, in the 1 to 534 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P13674-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- M I W Y I L I I G I L L P Q S L A H P G F F T S I G Q M T D L I H T E K D L V T S L K D Y I K A E E D K L E Q I K K W A E K L D R L T S T A T K D P E G F V G H P V N A F K L M K R L N T E W S E L E N L V L K D M S D G F I S N L T I Q R Q Y F P N D E D Q V G A A K A L L R L Q D T Y N L D T D T I S K G N L P G V K H K S F L T A E D C F E L G K V A Y T E A D Y Y H T E L W M E Q A L R Q L D E G E I S T I D K V S V L D Y L S Y A V Y Q Q G D L D K A L L L T K K L L E L D P E H Q R A N G N L K Y F E Y I M A K E K D V N K S A S D D Q S D Q K T T P K K K G V A V D Y L P E R Q K Y E M L C R G E G I K M T P R R Q K K L F C R Y H D G N R N P K F I L A P A K Q E D E W D K P R I I R F H D I I S D A E I E I V K D L A K P R L R R A T I S N P I T G D L E T V H Y R I S K S A W L S G Y E N P V V S R I N M R I Q D L T G L D V S T A E E L Q V A N Y G V G G Q Y E P H F D F A R K D E P D A F K E L G T G N R I A T W L F Y M S D V S A G G A T V F P E V G A S V W P K K G T A V F W Y N L F A S G E G D Y S T R H A A C P V L V G N K W V S N K W L H E R G Q E F R R P C T L S E L E
- Accession
- P13674
- Protein length
- Full Length
- Predicted molecular weight
- 84.81 kDa
- Amino acids
- 1 to 534
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
- Sequence similarities
Belongs to the P4HA family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein P4HA1 also known as Prolyl 4-Hydroxylase Subunit Alpha 1 functions mechanically by catalyzing the hydroxylation of proline residues in collagen. This activity requires iron and ascorbate as cofactors. P4HA1 is a part of the prolyl 4-hydroxylase enzyme complex and contains a molecular mass of approximately 63 kDa. It expresses widely in various human tissues with high expression in the liver skin and vascular tissues reflecting its role in collagen synthesis.
- Biological function summary
P4HA1 plays a central role in collagen biosynthesis and maturation by introducing hydroxyl groups into proline residues which stabilize the collagen triple helix. This protein functions as a critical component of the prolyl 4-hydroxylase complex which includes a beta subunit. Proper formation and stability of collagen fibers depend on P4HA1 activity impacting the structural integrity of connective tissues throughout the body.
- Pathways
P4HA1's function integrates into the pathways of collagen biosynthesis and modification. It is significant in the extracellular matrix organization pathway which upholds the structural support and cellular signaling. Additionally P4HA1 interacts with other proteins like COL1A1 and PLOD1 during these processes assisting in the proper formation of extracellular matrix structures essential for tissue function and injury repair.
- Associated diseases and disorders
P4HA1 has a strong association with fibrotic diseases such as liver fibrosis and pulmonary fibrosis where excessive collagen deposition is a hallmark. Dysregulation of P4HA1 may also play a role in tumor progression in cancers by promoting extracellular matrix remodeling therefore facilitating tumor invasion and metastasis. The protein interacts with pathways involving vascular endothelial growth factor (VEGF) which drives angiogenesis and is implicated in these disease processes.
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1 product image
SDS-PAGE - Recombinant Human P4HA1 protein (ab114624)
ab114624 analysed on a 12.5% SDS-PAGE stained with Coomassie Blue.
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