Recombinant Human p50 dynamitin protein is a Human Full Length protein, in the 1 to 406 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A D P K Y A D L P G I A R N E P D V Y E T S D L P E D D Q A E F D A F A Q E L E E L T S T S V E H I I V N P N A A Y D K F K D K R V G T K G L D F S D R I G K T K R T G Y E S G E Y E M L G E G L G V K E T P Q Q K Y Q R L L H E V Q E L T T E V E K I K T T V K E S A T E E K L T P V L L A K Q L A A L K Q Q L V A S H L E K L L G P D A A I N L T D P D G A L A K R L L L Q L E A T K N S K G G S G G K T T G T P P D S S L V T Y E L H S R P E Q D K F S Q A A K V A E L E K R L T E L E T A V R C D Q D A Q N P L S A G L Q G A C L M E T V E L L Q A K V S A L D L A V L D Q V E A R L Q S V L G K V N E I A K H K A S V E D A D T Q S K V H Q L Y E T I Q R W S P I A S T L P E L V Q R L V T I K Q L H E Q A M Q F G Q L L T H L D T T Q Q M I A N S L K D N T T L L T Q V Q T T M R E N L A T V E G N F A S I D E R M K K L G K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules. In the dynactin soulder domain, binds the ACTR1A filament and acts as a molecular ruler to determine the length (By similarity). Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development (By similarity).
Alternative names
DCTN50, DCTN2, Dynactin subunit 2, 50 kDa dynein-associated polypeptide, Dynactin complex 50 kDa subunit, p50 dynamitin, DCTN-50
Recommended products
Recombinant Human p50 dynamitin protein is a Human Full Length protein, in the 1 to 406 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q13561-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M A D P K Y A D L P G I A R N E P D V Y E T S D L P E D D Q A E F D A F A Q E L E E L T S T S V E H I I V N P N A A Y D K F K D K R V G T K G L D F S D R I G K T K R T G Y E S G E Y E M L G E G L G V K E T P Q Q K Y Q R L L H E V Q E L T T E V E K I K T T V K E S A T E E K L T P V L L A K Q L A A L K Q Q L V A S H L E K L L G P D A A I N L T D P D G A L A K R L L L Q L E A T K N S K G G S G G K T T G T P P D S S L V T Y E L H S R P E Q D K F S Q A A K V A E L E K R L T E L E T A V R C D Q D A Q N P L S A G L Q G A C L M E T V E L L Q A K V S A L D L A V L D Q V E A R L Q S V L G K V N E I A K H K A S V E D A D T Q S K V H Q L Y E T I Q R W S P I A S T L P E L V Q R L V T I K Q L H E Q A M Q F G Q L L T H L D T T Q Q M I A N S L K D N T T L L T Q V Q T T M R E N L A T V E G N F A S I D E R M K K L G K
- Accession
- Q13561
- Protein length
- Full Length
- Predicted molecular weight
- 47.2 kDa
- Amino acids
- 1 to 406
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab174453 is purified by using conventional chromatography techniques.
General info
- Function
Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules. In the dynactin soulder domain, binds the ACTR1A filament and acts as a molecular ruler to determine the length (By similarity). Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development (By similarity).
- Sequence similarities
Belongs to the dynactin subunit 2 family.
- Subcellular localisation
- Cytoskeleton, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Previously labelled as Dynamitin.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
P50 dynamitin also known as DCTN2 or dynactin subunit 2 is a protein component of the dynactin complex with a molecular mass of approximately 50 kDa. This protein is expressed in various tissues where it plays an important role in intracellular transport mechanisms. p50 dynamitin is essential for the proper functioning of the dynein-dynactin motor protein complex which is important for the transport of organelles and vesicles along microtubules within cells.
- Biological function summary
P50 dynamitin serves as a regulator within the dynactin complex ensuring its structural integrity and facilitating interaction with dynein motor proteins. It is part of the larger dynactin complex which consists of over 20 subunits. p50 dynamitin's role in this complex directly impacts cytoplasmic and nuclear activities including the trafficking of endosomes lysosomes and other cargo within cells. The proper functioning of this complex is necessary for maintaining the correct intracellular transport which affects various cellular processes.
- Pathways
P50 dynamitin is involved in the cytoskeletal network through its interaction with the dynein motor protein pathway and is closely related to microtubule dynamics. Dynactin of which p50 is a part functions with dynein to mediate minus-end-directed movement along microtubules necessary for processes such as mitotic spindle formation and chromosome alignment. This relationship with dynein highlights its role in cell division and intracellular transport impacting neurological pathway regulation.
- Associated diseases and disorders
P50 dynamitin has been linked to neurological disorders like motor neuron disease and amyotrophic lateral sclerosis (ALS). The dysfunction of the dynactin-dynein complex where p50 dynamitin plays a regulatory role can lead to disrupted neuronal transport processes contributing to these diseases. Additionally its association with the protein TDP-43 points to a connection with neurodegenerative conditions further illustrating its clinical significance in the pathology of ALS and related disorders.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human p50 dynamitin protein (ab174453)
15% SDS-PAGE analysis of ab174453 (3 μg)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com