Recombinant Human p53 protein is a Human Full Length protein, in the 1 to 393 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Publications
Filter by
- Biosensors 12:2022Detection and Quantification of Tp53 and p53-Anti-p53 Autoantibody Immune Complex: Promising Biomarkers in Early Stage Lung Cancer Diagnosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeum-Soo Song,Satish Balasaheb Nimse,Shrikant Dashrath Warkad,Jung-Hoon Kim,Hey-Jin Kim,Taisun KimPubMed 35200387
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
6x His tag N-Terminus
SUMO tag N-Terminus- Applications
SDS-PAGE, MS
- Biologically active
No
Amino acid sequence
M E E P Q S D P S V E P P L S Q E T F S D L W K L L P E N N V L S P L P S Q A M D D L M L S P D D I E Q W F T E D P G P D E A P R M P E A A P P V A P A P A A P T P A A P A P A P S W P L S S S V P S Q K T Y Q G S Y G F R L G F L H S G T A K S V T C T Y S P A L N K M F C Q L A K T C P V Q L W V D S T P P P G T R V R A M A I Y K Q S Q H M T E V V R R C P H H E R C S D S D G L A P P Q H L I R V E G N L R V E Y L D D R N T F R H S V V V P Y E P P E V G S D C T T I H Y N Y M C N S S C M G G M N R R P I L T I I T L E D S S G N L L G R N S F E V R V C A C P G R D R R T E E E N L R K K G E P H H E L P P G S T K R A L P N N T S S S P Q P K K K P L D G E Y F T L Q I R G R E R F E M F R E L N E A L E L K D A Q A G K E P G G S R A H S S H L K S K K G Q S T S R H K K L M F K T E G P D S D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed:12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed:12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
Alternative names
Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, P53, TP53
Recommended products
Recombinant Human p53 protein is a Human Full Length protein, in the 1 to 393 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
6x His tag N-Terminus
SUMO tag N-Terminus- Applications
SDS-PAGE, MS
- Biologically active
No
- Accession
- P04637-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
M E E P Q S D P S V E P P L S Q E T F S D L W K L L P E N N V L S P L P S Q A M D D L M L S P D D I E Q W F T E D P G P D E A P R M P E A A P P V A P A P A A P T P A A P A P A P S W P L S S S V P S Q K T Y Q G S Y G F R L G F L H S G T A K S V T C T Y S P A L N K M F C Q L A K T C P V Q L W V D S T P P P G T R V R A M A I Y K Q S Q H M T E V V R R C P H H E R C S D S D G L A P P Q H L I R V E G N L R V E Y L D D R N T F R H S V V V P Y E P P E V G S D C T T I H Y N Y M C N S S C M G G M N R R P I L T I I T L E D S S G N L L G R N S F E V R V C A C P G R D R R T E E E N L R K K G E P H H E L P P G S T K R A L P N N T S S S P Q P K K K P L D G E Y F T L Q I R G R E R F E M F R E L N E A L E L K D A Q A G K E P G G S R A H S S H L K S K K G Q S T S R H K K L M F K T E G P D S D
- Accession
- Protein length
Full Length
- Predicted molecular weight
59.7 kDa
- Amino acids
1 to 393
- Nature
Recombinant
- Tags
6x His tag N-Terminus, SUMO tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 (PubMed:12524540). However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP (PubMed:12524540). In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
- Sequence similarities
Belongs to the p53 family.
- Post-translational modifications
Acetylated. Acetylation of Lys-382 by CREBBP enhances transcriptional activity. Deacetylation of Lys-382 by SIRT1 impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylation by SIRT2 impairs its ability to induce transcription activation in a AKT-dependent manner.
- Subcellular localisation
Nucleus, PML body, Mitochondrion matrix, Cytoskeleton, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein p53 also known as TP53 or tumor protein p53 has a molecular weight of approximately 53 kDa. It acts as a transcription factor and plays a major role in cell cycle regulation apoptosis and maintaining genomic stability. This protein mainly expresses in the nucleus of cells and acts as a critical regulator of cellular responses to stress signals including DNA damage. Scientists commonly use p53 antibodies in various assays like western blot and p53 immunofluorescence to detect and study its expression and functional status in cells.
- Biological function summary
P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.
- Pathways
P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.
- Associated diseases and disorders
P53 mutation or inactivation is often associated with the development of cancer given its role in controlling cell division and preventing tumor formation. Specifically its dysfunction has been linked to cancers such as breast cancer and lung cancer. Additionally p53 can interact with other mutant proteins like Ras compounding mutations that contribute to tumor progression and aggressive cancer phenotypes. Understanding these interactions and the status of p53 can be important in developing targeted cancer therapies.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
SDS-PAGE - Recombinant Human p53 protein (ab237007)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel analysis of ab237007.
Mass Spectrometry - Recombinant Human p53 protein (ab237007)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab237007 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) TP53.
Mass Spectrometry - Recombinant Human p53 protein (ab237007)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab237007 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) TP53.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com