Recombinant human P70 S6 Kinase beta/SRK protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >95% purity and suitable for WB, FuncS.
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Publications
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- The EMBO journal 31:2365-772012Chk1 phosphorylates the tumour suppressor Mig-6, regulating the activation of EGF signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNing Liu et. al.PubMed 22505024
Key facts
- Purity
>95% Densitometry
- Expression system
Baculovirus infected Sf9 cells
- Tags
Tag free
- Applications
WB, FuncS
- Biologically active
Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Phosphorylates specifically ribosomal protein S6 (PubMed:29750193). Seems to act downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression in an alternative pathway regulated by MEAK7 (PubMed:29750193).
Alternative names
STK14B, RPS6KB2, Ribosomal protein S6 kinase beta-2, S6K-beta-2, S6K2, 70 kDa ribosomal protein S6 kinase 2, S6 kinase-related kinase, Serine/threonine-protein kinase 14B, p70 ribosomal S6 kinase beta, P70S6K2, p70-S6K 2, SRK, S6K-beta, p70 S6 kinase beta, p70 S6K-beta, p70 S6KB, p70-beta
Recommended products
Recombinant human P70 S6 Kinase beta/SRK protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >95% purity and suitable for WB, FuncS.
Key facts
- Purity
>95% Densitometry
- Expression system
Baculovirus infected Sf9 cells
- Tags
Tag free
- Applications
WB, FuncS
- Biologically active
Yes
- Biological activity
Specific activity: 107nmol/min/mg.
- Accession
- Q9UBS0-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF
Specifications
- Form
Liquid
- Additional notes
Affinity purified.
General info
- Function
Phosphorylates specifically ribosomal protein S6 (PubMed:29750193). Seems to act downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression in an alternative pathway regulated by MEAK7 (PubMed:29750193).
- Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
- Post-translational modifications
Phosphorylated and activated by MTOR. Phosphorylation by PKC within the NLS in response to mitogenic stimuli causes cytoplasmic retention.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-80°C
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
RPS6 peptide ab204879 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
Previously labelled as P70 S6 Kinase beta.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
P70 S6 Kinase beta also known as SRK is a protein kinase involved in the regulation of cellular growth and metabolism. It has a molecular mass of approximately 70 kDa. This kinase is predominantly expressed in brain liver and muscle tissues. P70 is an important mediator in the process of protein translation acting mechanically by phosphorylating the ribosomal protein S6 which in turn enhances the translation of a subset of mRNAs related to cell growth processes.
- Biological function summary
P70 S6 Kinase beta/SRK is a significant component in the cellular signaling complexes that manage cell size and energy regulation. It participates in the mechanistic target of rapamycin (mTOR) pathway. Through its enzymatic activity it helps cells respond to growth factors and nutrients. By motivating the translation of mRNAs p70 sustains ribosome biogenesis and cell growth.
- Pathways
P70 operates within the mTOR signaling pathway known for its role in controlling cell growth and proliferation. It interacts with proteins like mTORC1 which is a known regulator of cellular metabolism and protein synthesis. These interactions allow p70 to integrate signals from other kinases like Akt influencing downstream responses including glucose metabolism.
- Associated diseases and disorders
P70 S6 Kinase beta/SRK links to conditions such as cancer and type 2 diabetes. Aberrant activation of P70 through the mTOR pathway contributes significantly to tumorigenesis due to its influence on cell growth and survival. Additionally in type 2 diabetes dysregulated signaling of P70 affects insulin sensitivity often in conjunction with related enzymes such as Akt and mTOR.
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2 product images
Functional Studies - Recombinant human P70 S6 Kinase beta/SRK protein (ab84798)
specific activity of ab84798
SDS-PAGE - Recombinant human P70 S6 Kinase beta/SRK protein (ab84798)
SDS Page analysis of ab84798
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