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AB101716

Recombinant Human p73 alpha protein

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Recombinant Human p73 alpha protein is a Human Full Length protein, in the 1 to 636 aa range, expressed in Baculovirus, with >90%, suitable for SDS-PAGE.

View Alternative Names

P73, TP73, Tumor protein p73, p53-like transcription factor, p53-related protein

1 Images
SDS-PAGE - Recombinant Human p73 alpha protein (AB101716)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human p73 alpha protein (AB101716)

The putity of ab101716 was determined to be 90% by densitometry.

Approximate MWt : 110kDa

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O15350

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MAQSTATSPDGGTTFEHLWSSLEPDSTYFDLPQSSRGNNEVVGGTDSSMDVFHLEGMTTSVMAQFNLLSSTMDQMSSRAASASPYTPEHAASVPTHSPYAQPSSTFDTMSPAPVIPSNTDYPGPHHFEVTFQQSSTAKSATWTYSPLLKKLYCQIAKTCPIQIKVSTPPPPGTAIRAMPVYKKAEHVTDVVKRCPNHELGRDFNEGQSAPASHLIRVEGNNLSQYVDDPVTGRQSVVVPYEPPQVGTEFTTILYNFMCNSSCVGGMNRRPILIIITLEMRDGQVLGRRSFEGRICACPGRDRKADEDHYREQQALNESSAKNGAASKRAFKQSPPAVPALGAGVKKRRHGDEDTYYLQVRGRENFEILMKLKESLELMELVPQPLVDSYRQQQQLLQRPSHLQPPSYGPVLSPMNKVHGGMNKLPSVNQLVGQPPPHSSAATPNLGPVGPGMLNNHGHAVPANGEMSSSHSAQSMVSGSHCTPPPPYHADPSLVSFLTGLGCPNCIEYFTSQGLQSIYHLQNLTIEDLGALKIPEQYRMTIWRGLQDLKQGHDYSTAQQLLRSSNAATISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGPGGGPDEWADFGFDLPDCKARKQPIKEEFTEAEIH","proteinLength":"Full Length","predictedMolecularWeight":"110 kDa","actualMolecularWeight":null,"aminoAcidEnd":636,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus","accessionNumber":"O15350","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

P73 alpha also known as TP73 or tumor protein 73 functions mechanically as a transcription factor. It belongs to the p53 family of proteins and boasts a mass of approximately 80 kDa. This protein localizes in the nucleus and cytoplasm of cells. Expression of p73 alpha occurs in various tissues including the brain and lymphoid tissues. Researchers have identified several isotypes with p73 alpha being one of the more significant forms due to its role in regulating numerous genes involved in cell cycle and apoptosis.
Biological function summary

P73 alpha influences cellular processes such as apoptosis differentiation and cell cycle regulation. It does not work alone; rather it is part of a complex that includes other family members like p63 and p53 contributing to genomic stability. The protein's ability to activate p53-responsive genes highlights its function as an important player in tumor suppression. p73 alpha's roles extend beyond cancer suppression as it participates in neural development and tissue homeostasis.

Pathways

P73 alpha operates critically within the p53 signaling pathway maintaining the balance between DNA repair and cell death. It interacts with proteins like MDM2 and BAX which are essential in these pathways. Additionally p73 alpha takes part in the Notch signaling pathway which influences differentiation and development. These interactions emphasize p73 alpha's involvement in safeguarding cellular responses to stress and maintaining cellular integrity.

P73 alpha's malfunction relates prominently to cancer and neurodegenerative disorders. Abnormalities in its expression or function associate with various cancers including breast and ovarian where it interacts with other proteins like p53 impacting tumor growth and progression. Similarly dysfunction in its pathways associates with neurodegenerative diseases highlighting a link with proteins such as Tau which implicates it in neuronal death and deterioration in conditions like Alzheimer's disease.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. Is an activator of FOXJ1 expression (By similarity). It is an essential factor for the positive regulation of lung ciliated cell differentiation (PubMed : 34077761).

Sequence similarities

Belongs to the p53 family.

Post-translational modifications

Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity. Phosphorylation by PLK1 and PLK3 inhibits the transcription regulator activity and pro-apoptotic function.. Higher levels of phosphorylation seen in the brain from patients with Huntington disease.. Polyubiquitinated by RCHY1/PIRH2; leading to its degradation by the proteasome.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. Is an activator of FOXJ1 expression (By similarity). It is an essential factor for the positive regulation of lung ciliated cell differentiation (PubMed : 34077761).
See full target information TP73
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