Recombinant Human PABPN1 protein (denatured) (His tag N-Terminus)
Be the first to review this product! Submit a review
|
(0 Publication)
Recombinant Human PABPN1 protein (denatured) (His tag N-Terminus) is a Human Fragment protein, in the 119 to 306 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.
View Alternative Names
PAB2, PABP2, PABPN1, Polyadenylate-binding protein 2, PABP-2, Poly(A)-binding protein 2, Nuclear poly(A)-binding protein 1, Poly(A)-binding protein II, Polyadenylate-binding nuclear protein 1, PABII
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human PABPN1 protein (denatured) (His tag N-Terminus) (AB177635)
15% SDS-PAGE analysis of ab177635 (3μg).
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PABPN1 is involved in controlling the polyadenylation process of pre-mRNA which is essential for producing mature mRNA molecules. PABPN1 operates within a multi-protein complex that includes enzymes like poly(A) polymerase and cleavage stimulation factor (CstF). By interacting with these proteins PABPN1 ensures the correct formation of the 3' end of mRNA influencing gene expression regulation and mRNA stability.
Pathways
PABPN1 participates in key cellular pathways such as mRNA processing and nuclear export of mRNA. It operates in concert with other proteins like PABPC1 which aids in the transition of mRNA from the nucleus to the cytoplasm. The protein maintains the balance of mRNA stability and translation efficiency which are critical aspects of efficient gene expression.
Specifications
Form
Liquid
General info
Function
Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product (By similarity). Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length (By similarity). Increases the affinity of poly(A) polymerase for RNA (By similarity). Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes (PubMed : 11371506). Binds to poly(A) and to poly(G) with high affinity (By similarity). May protect the poly(A) tail from degradation (By similarity). Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed : 27871484).
Post-translational modifications
Arginine dimethylation is asymmetric and involves PRMT1 and PRMT3. It does not influence the RNA binding properties (By similarity).
Subcellular localisation
Nucleus
Target data
Complementary Products
![Flow Cytometry (Intracellular) - Anti-PABPN1 antibody [EP3000Y] (AB75855)](https://content.abcam.com/products/images/pabpn1-antibody-ep3000y-ab75855--flow-cytometry-intracellular-img13477.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com