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Recombinant Human PABPN1 protein (denatured) is a Human Fragment protein, in the 119 to 306 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Alternative names=Polyadenylate-binding protein 2, PABP-2, Poly(A)-binding protein 2, Nuclear poly(A)-binding protein 1, Poly(A)-binding protein II, Polyadenylate-binding nuclear protein 1, PABII, PABP2, PAB2, PABPN1
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Sequence
M G S S H H H H H H S S G L V P R G S H M G S L E A I K A R V R E M E E E A E K L K E L Q N E V E K Q M N M S P P P G N A G P V I M S I E E K M E A D A R S I Y V G N V D Y G A T A E E L E A H F H G C G S V N R V T I L C D K F S G H P K G F A Y I E F S D K E S V R T S L A L D E S L F R G R Q I K V I P K R T N R P G I S T T D R G F P R A R Y R A R T T N Y N S S R S R F Y S G F N S R P R G R V Y R G R A R A T S W Y S P Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant Human PABPN1 protein (denatured) is a Human Fragment protein, in the 119 to 306 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Alternative names=Polyadenylate-binding protein 2, PABP-2, Poly(A)-binding protein 2, Nuclear poly(A)-binding protein 1, Poly(A)-binding protein II, Polyadenylate-binding nuclear protein 1, PABII, PABP2, PAB2, PABPN1
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Predicted molecular weight
23.8 kDa
- Amino acids
119 to 306
- Sequence
M G S S H H H H H H S S G L V P R G S H M G S L E A I K A R V R E M E E E A E K L K E L Q N E V E K Q M N M S P P P G N A G P V I M S I E E K M E A D A R S I Y V G N V D Y G A T A E E L E A H F H G C G S V N R V T I L C D K F S G H P K G F A Y I E F S D K E S V R T S L A L D E S L F R G R Q I K V I P K R T N R P G I S T T D R G F P R A R Y R A R T T N Y N S S R S R F Y S G F N S R P R G R V Y R G R A R A T S W Y S P Y
- Accession
- Q86U42-1
- Protein length
Fragment
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Specifications
- Form
Liquid
General info
- Function
Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product (By similarity). Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length (By similarity). Increases the affinity of poly(A) polymerase for RNA (By similarity). Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes (PubMed:11371506). Binds to poly(A) and to poly(G) with high affinity (By similarity). May protect the poly(A) tail from degradation (By similarity). Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed:27871484).
- Post-translational modifications
Arginine dimethylation is asymmetric and involves PRMT1 and PRMT3. It does not influence the RNA binding properties (By similarity).
- Subcellular localisation
Nucleus, Nucleus speckle
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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