Skip to main content

Recombinant Human PAC-2 protein is a Human Full Length protein, in the 1 to 264 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.

Be the first to review this product! Submit a review

Images

SDS-PAGE - Recombinant Human PAC-2 protein (AB113597), expandable thumbnail

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, MS

Biologically active

No

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M G S M F V P C G E S A P D L A G F T L L M P A V S V G N V G Q L A M D L I I S T L N M S K I G Y F Y T D C L V P M V G N N P Y A T T E G N S T E L S I N A E V Y S L P S R K L V A L Q L R S I F I K Y K S K P F C E K L L S W V K S S G C A R V I V L S S S H S Y Q R N D L Q L R S T P F R Y L L T P S M Q K S V Q N K I K S L N W E E M E K S R C I P E I D D S E F C I R I P G G G I T K T L Y D E S C S K E I Q M A V L L K F V S E G D N I P D A L G L V E Y L N E W L Q I L K P L S D D P T V S A S R W K I P S S W R L L F G S G L P P A L F

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.

Alternative names

Recommended products

Recombinant Human PAC-2 protein is a Human Full Length protein, in the 1 to 264 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, MS

Accession
Q969U7-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 40% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl

Sequence info

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M G S M F V P C G E S A P D L A G F T L L M P A V S V G N V G Q L A M D L I I S T L N M S K I G Y F Y T D C L V P M V G N N P Y A T T E G N S T E L S I N A E V Y S L P S R K L V A L Q L R S I F I K Y K S K P F C E K L L S W V K S S G C A R V I V L S S S H S Y Q R N D L Q L R S T P F R Y L L T P S M Q K S V Q N K I K S L N W E E M E K S R C I P E I D D S E F C I R I P G G G I T K T L Y D E S C S K E I Q M A V L L K F V S E G D N I P D A L G L V E Y L N E W L Q I L K P L S D D P T V S A S R W K I P S S W R L L F G S G L P P A L F

Accession

Q969U7

Protein length

Full Length

Predicted molecular weight

31.8 kDa

Amino acids

1 to 264

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

Additional notes

ab113597 was purified using conventional chromatography.

General info

Function

Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.

Sequence similarities

Belongs to the PSMG2 family.

Post-translational modifications

Degraded by the proteasome upon completion of 20S proteasome maturation.

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PAC-2 also known as PAC and Anti-PAC is a protein involved in various cellular processes. It appears important in apoptosis and the regulation of cellular proliferation. PAC-2 has an approximate mass of 40 kDa. Researchers have observed its expression in different tissues including the nervous system immune cells and several cancerous tissues. Understanding the mechanical functions of PAC-2 can offer insights into its role in cellular homeostasis and disease pathology.

Biological function summary

PAC-2 plays an essential role in apoptosis acting as a mediator of programmed cell death. While not characterized as part of a larger complex PAC-2 interacts with other proteins to regulate cell survival and death. It likely influences the mitochondrial pathway of apoptosis by controlling cytochrome c release which is a critical step in the activation of caspases. These interactions emphasize the protein's adaptive importance in various biological contexts.

Pathways

PAC-2 commands a significant presence in apoptotic signaling and cellular stress response pathways. It influences the intrinsic (mitochondrial) apoptotic pathway and may interact with proteins such as Bcl-2 family members and caspase-9. This interaction places PAC-2 in an important position where it can modify the balance between cell survival and cell death involving proteins that balance pro-apoptotic and anti-apoptotic signals.

Associated diseases and disorders

PAC-2 has correlations with cancer and neurodegenerative diseases. Its dysregulation in certain cancers suggests a role in tumor progression and resistance to apoptosis. The connection to Bcl-2 highlights this aspect as both are involved in the suppression of cell death. In neurodegenerative diseases like Alzheimer's altered PAC-2 function might contribute to the pathological accumulation of damaged cells influenced by its effect on apoptotic pathways. Understanding these links could advance therapeutic strategies for related conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • SDS-PAGE - Recombinant Human PAC-2 protein (ab113597), expandable thumbnail

    SDS-PAGE - Recombinant Human PAC-2 protein (ab113597)

    15% SDS-PAGE analysis of ab113597 (3μg)

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com