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AB113597

Recombinant Human PAC-2 protein

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Recombinant Human PAC-2 protein is a Human Full Length protein, in the 1 to 264 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

HCCA3, PAC2, TNFSF5IP1, PSMG2, Proteasome assembly chaperone 2, PAC-2, Hepatocellular carcinoma-susceptibility protein 3, Tumor necrosis factor superfamily member 5-induced protein 1

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SDS-PAGE - Recombinant Human PAC-2 protein (AB113597)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human PAC-2 protein (AB113597)

15% SDS-PAGE analysis of ab113597 (3μg)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q969U7

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMFVPCGESAPDLAGFTLLMPAVSVGNVGQLAMDLIISTLNMSKIGYFYTDCLVPMVGNNPYATTEGNSTELSINAEVYSLPSRKLVALQLRSIFIKYKSKPFCEKLLSWVKSSGCARVIVLSSSHSYQRNDLQLRSTPFRYLLTPSMQKSVQNKIKSLNWEEMEKSRCIPEIDDSEFCIRIPGGGITKTLYDESCSKEIQMAVLLKFVSEGDNIPDALGLVEYLNEWLQILKPLSDDPTVSASRWKIPSSWRLLFGSGLPPALF","proteinLength":"Full Length","predictedMolecularWeight":"31.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":264,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q969U7","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PAC-2 also known as PAC and Anti-PAC is a protein involved in various cellular processes. It appears important in apoptosis and the regulation of cellular proliferation. PAC-2 has an approximate mass of 40 kDa. Researchers have observed its expression in different tissues including the nervous system immune cells and several cancerous tissues. Understanding the mechanical functions of PAC-2 can offer insights into its role in cellular homeostasis and disease pathology.
Biological function summary

PAC-2 plays an essential role in apoptosis acting as a mediator of programmed cell death. While not characterized as part of a larger complex PAC-2 interacts with other proteins to regulate cell survival and death. It likely influences the mitochondrial pathway of apoptosis by controlling cytochrome c release which is a critical step in the activation of caspases. These interactions emphasize the protein's adaptive importance in various biological contexts.

Pathways

PAC-2 commands a significant presence in apoptotic signaling and cellular stress response pathways. It influences the intrinsic (mitochondrial) apoptotic pathway and may interact with proteins such as Bcl-2 family members and caspase-9. This interaction places PAC-2 in an important position where it can modify the balance between cell survival and cell death involving proteins that balance pro-apoptotic and anti-apoptotic signals.

PAC-2 has correlations with cancer and neurodegenerative diseases. Its dysregulation in certain cancers suggests a role in tumor progression and resistance to apoptosis. The connection to Bcl-2 highlights this aspect as both are involved in the suppression of cell death. In neurodegenerative diseases like Alzheimer's altered PAC-2 function might contribute to the pathological accumulation of damaged cells influenced by its effect on apoptotic pathways. Understanding these links could advance therapeutic strategies for related conditions.
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Specifications

Form

Liquid

Additional notes

ab113597 was purified using conventional chromatography.

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General info

Function

Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.

Sequence similarities

Belongs to the PSMG2 family.

Post-translational modifications

Degraded by the proteasome upon completion of 20S proteasome maturation.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.
See full target information PSMG2
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Complementary Products

Primary Antibodies

AB183103

Anti-PACAP receptor/ADCYAP1R1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP receptor/ADCYAP1R1 antibody (AB183103)

  • 0
  • 0
Primary Antibodies

AB172909

Anti-PAC-2 antibody [EPR9947(2)]

RabMAb|Recombinant

Western blot - Anti-PAC-2 antibody [EPR9947(2)] (AB172909)

  • 5
  • 1
Primary Antibodies

AB181205

Anti-PACAP antibody [EPR11544]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP antibody [EPR11544] (AB181205)

  • 0
  • 0
Proteins & Peptides

AB276678

Recombinant Human PACAP protein (Fc Chimera)

SDS-PAGE - Recombinant Human PACAP protein (Fc Chimera) (AB276678)

  • 0
  • 0

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