Recombinant human PADI1 / PAD1 protein is a Human Full Length protein, in the 1 to 663 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M A P K R V V Q L S L K M P T H A V C V V G V E A H V D I H S D V P K G A N S F R V S G S S G V E V F M V Y N R T R V K E P I G K A R W P L D T D A D M V V S V G T A S K E L K D F K V R V S Y F G E Q E D Q A L G R S V L Y L T G V D I S L E V D T G R T G K V K R S Q G D K K T W R W G P E G Y G A I L L V N C D R D N H R S A E P D L T H S W L M S L A D L Q D M S P M L L S C N G P D K L F D S H K L V L N V P F S D S K R V R V F C A R G G N S L S D Y K Q V L G P Q C L S Y E V E R Q P G E Q E I K F Y V E G L T F P D A D F L G L V S L S V S L V D P G T L P E V T L F T D T V G F R M A P W I M T P N T Q P P E E L Y V C R V M D T H G S N E K F L E D M S Y L T L K A N C K L T I C P Q V E N R N D R W I Q D E M E F G Y I E A P H K S F P V V F D S P R N R G L K D F P Y K R I L G P D F G Y V T R E I P L P G P S S L D S F G N L D V S P P V T V G G T E Y P L G R I L I G S S F P K S G G R Q M A R A V R N F L K A Q Q V Q A P V E L Y S D W L S V G H V D E F L T F V P T S D Q K G F R L L L A S P S A C L K L F Q E K K E E G Y G E A A Q F D G L K H Q A K R S I N E M L A D R H L Q R D N L H A Q K C I D W N R N V L K R E L G L A E S D I V D I P Q L F F L K N F Y A E A F F P D M V N M V V L G K Y L G I P K P Y G P I I N G R C C L E E K V Q S L L E P L G L H C I F I D D Y L S Y H E L Q G E I H C G T N V R R K P F P F K W W N M V P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the deimination of arginine residues of proteins.
Alternative names
PAD1, PDI1, PADI1, Protein-arginine deiminase type-1, Peptidylarginine deiminase I, Protein-arginine deiminase type I
Recommended products
Recombinant human PADI1 / PAD1 protein is a Human Full Length protein, in the 1 to 663 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: ≥35 pmol/min/μg
Assay Conditions: Enzyme reaction was performed in a buffer (50 mM Tris, pH 7.4, 50 mM NaCl, 10 mM CaCl2, and 2 mM DTT) containing ab196400 and 10 mM BAEE as a substrate for 25 min at 37°C. Amount of ammonia produced was measured and used for specific activity calculation. - Accession
- Q9ULC6-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.72% Sodium chloride, 0.71% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M A P K R V V Q L S L K M P T H A V C V V G V E A H V D I H S D V P K G A N S F R V S G S S G V E V F M V Y N R T R V K E P I G K A R W P L D T D A D M V V S V G T A S K E L K D F K V R V S Y F G E Q E D Q A L G R S V L Y L T G V D I S L E V D T G R T G K V K R S Q G D K K T W R W G P E G Y G A I L L V N C D R D N H R S A E P D L T H S W L M S L A D L Q D M S P M L L S C N G P D K L F D S H K L V L N V P F S D S K R V R V F C A R G G N S L S D Y K Q V L G P Q C L S Y E V E R Q P G E Q E I K F Y V E G L T F P D A D F L G L V S L S V S L V D P G T L P E V T L F T D T V G F R M A P W I M T P N T Q P P E E L Y V C R V M D T H G S N E K F L E D M S Y L T L K A N C K L T I C P Q V E N R N D R W I Q D E M E F G Y I E A P H K S F P V V F D S P R N R G L K D F P Y K R I L G P D F G Y V T R E I P L P G P S S L D S F G N L D V S P P V T V G G T E Y P L G R I L I G S S F P K S G G R Q M A R A V R N F L K A Q Q V Q A P V E L Y S D W L S V G H V D E F L T F V P T S D Q K G F R L L L A S P S A C L K L F Q E K K E E G Y G E A A Q F D G L K H Q A K R S I N E M L A D R H L Q R D N L H A Q K C I D W N R N V L K R E L G L A E S D I V D I P Q L F F L K N F Y A E A F F P D M V N M V V L G K Y L G I P K P Y G P I I N G R C C L E E K V Q S L L E P L G L H C I F I D D Y L S Y H E L Q G E I H C G T N V R R K P F P F K W W N M V P
- Accession
- Q9ULC6
- Protein length
- Full Length
- Predicted molecular weight
- 75 kDa
- Amino acids
- 1 to 663
- Nature
- Recombinant
- Tags
- DDDDK tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Catalyzes the deimination of arginine residues of proteins.
- Sequence similarities
Belongs to the protein arginine deiminase family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PADI1 also known as PAD1 or PADIone is a protein of approximately 74 kDa that belongs to the peptidylarginine deiminase family. It catalyzes the conversion of arginine residues into citrulline in a calcium-dependent manner a process known as citrullination or deimination. The PADI1 expression is mainly in the epidermis hair follicles and other keratinizing tissues. The enzyme requires at least one Ca2+ ion for its activity and often associates with the nucleus and cytoplasm within cells.
- Biological function summary
PADI1 plays an important role in modulating structural changes in proteins involved in skin differentiation and hair formation. It is not known to form part of a larger protein complex but instead acts as a standalone enzyme in multiple cellular processes. The citrullination activity of PADI1 significantly affects the structural proteins like keratin and filaggrin influencing keratinocyte terminal differentiation and barrier function of the skin.
- Pathways
PADI1 is instrumental in the formation of the epidermal barrier by contributing to the terminal differentiation pathway of keratinocytes. This pathway ensures the proper development and maintenance of the skin layers. It associates with proteins such as keratin and filaggrin during this process. Furthermore PADI1 also links to pathways involved in the regulation of intracellular protein modification impacting the overall protein structure and function in tissues.
- Associated diseases and disorders
PADI1 has been linked to psoriasis and skin cancers. In psoriasis the aberrant expression or regulation of PADI1 can lead to improper protein citrullination affecting skin barrier function and immune responses. Moreover dysregulated activity of PADI1 connects to skin cancer progression through its impact on keratinocyte proliferation and differentiation. Research has shown interactions with other proteins like keratin as part of these disease processes relevant to its role in skin pathology.
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2 product images
Functional Studies - Recombinant human PADI1 / PAD1 protein (ab196400)
Example of specific activity of ab196400
SDS-PAGE - Recombinant human PADI1 / PAD1 protein (ab196400)
10% SDS-PAGE analysis of ab196400 (5 μg) with Coomassie staining.
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