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AB196400

Recombinant human PADI1 / PAD1 protein (DDDDK tag C-Terminus)

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Recombinant human PADI1 / PAD1 protein (DDDDK tag C-Terminus) is a Human Full Length protein, in the 1 to 663 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.

View Alternative Names

PAD1, PDI1, PADI1, Protein-arginine deiminase type-1, Peptidylarginine deiminase I, Protein-arginine deiminase type I

2 Images
Functional Studies - Recombinant human PADI1 / PAD1 protein (DDDDK tag C-Terminus) (AB196400)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PADI1 / PAD1 protein (DDDDK tag C-Terminus) (AB196400)

Example of specific activity of ab196400

SDS-PAGE - Recombinant human PADI1 / PAD1 protein (DDDDK tag C-Terminus) (AB196400)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PADI1 / PAD1 protein (DDDDK tag C-Terminus) (AB196400)

10% SDS-PAGE analysis of ab196400 (5 μg) with Coomassie staining.

Key facts

Purity

>70% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

DDDDK tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific Activity: ≥35 pmol/min/μg

Assay Conditions: Enzyme reaction was performed in a buffer (50 mM Tris, pH 7.4, 50 mM NaCl, 10 mM CaCl2, and 2 mM DTT) containing ab196400 and 10 mM BAEE as a substrate for 25 min at 37°C. Amount of ammonia produced was measured and used for specific activity calculation.

Accession

Q9ULC6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.72% Sodium chloride, 0.71% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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Complementary Products

Primary Antibodies

AB181762

Anti-PADI1 / PAD1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI1 / PAD1 antibody (AB181762)

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Primary Antibodies

AB201172

Anti-Glutaminyl cyclase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminyl cyclase antibody (AB201172)

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Proteins & Peptides

AB313361

Recombinant Human WFDC2 Protein (His tag)

Mass Spectrometry - Recombinant Human WFDC2 Protein (His tag) (AB313361)

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  • 0
Proteins & Peptides

AB95933

Recombinant human XIAP protein (DDDDK tag N-Terminus)

Functional Studies - Recombinant human XIAP protein (DDDDK tag N-Terminus) (AB95933)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
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Sequence info

[{"sequence":"MAPKRVVQLSLKMPTHAVCVVGVEAHVDIHSDVPKGANSFRVSGSSGVEVFMVYNRTRVKEPIGKARWPLDTDADMVVSVGTASKELKDFKVRVSYFGEQEDQALGRSVLYLTGVDISLEVDTGRTGKVKRSQGDKKTWRWGPEGYGAILLVNCDRDNHRSAEPDLTHSWLMSLADLQDMSPMLLSCNGPDKLFDSHKLVLNVPFSDSKRVRVFCARGGNSLSDYKQVLGPQCLSYEVERQPGEQEIKFYVEGLTFPDADFLGLVSLSVSLVDPGTLPEVTLFTDTVGFRMAPWIMTPNTQPPEELYVCRVMDTHGSNEKFLEDMSYLTLKANCKLTICPQVENRNDRWIQDEMEFGYIEAPHKSFPVVFDSPRNRGLKDFPYKRILGPDFGYVTREIPLPGPSSLDSFGNLDVSPPVTVGGTEYPLGRILIGSSFPKSGGRQMARAVRNFLKAQQVQAPVELYSDWLSVGHVDEFLTFVPTSDQKGFRLLLASPSACLKLFQEKKEEGYGEAAQFDGLKHQAKRSINEMLADRHLQRDNLHAQKCIDWNRNVLKRELGLAESDIVDIPQLFFLKNFYAEAFFPDMVNMVVLGKYLGIPKPYGPIINGRCCLEEKVQSLLEPLGLHCIFIDDYLSYHELQGEIHCGTNVRRKPFPFKWWNMVP","proteinLength":"Full Length","predictedMolecularWeight":"75 kDa","actualMolecularWeight":null,"aminoAcidEnd":663,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9ULC6","tags":[{"tag":"DDDDK","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PADI1 also known as PAD1 or PADIone is a protein of approximately 74 kDa that belongs to the peptidylarginine deiminase family. It catalyzes the conversion of arginine residues into citrulline in a calcium-dependent manner a process known as citrullination or deimination. The PADI1 expression is mainly in the epidermis hair follicles and other keratinizing tissues. The enzyme requires at least one Ca2+ ion for its activity and often associates with the nucleus and cytoplasm within cells.
Biological function summary

PADI1 plays an important role in modulating structural changes in proteins involved in skin differentiation and hair formation. It is not known to form part of a larger protein complex but instead acts as a standalone enzyme in multiple cellular processes. The citrullination activity of PADI1 significantly affects the structural proteins like keratin and filaggrin influencing keratinocyte terminal differentiation and barrier function of the skin.

Pathways

PADI1 is instrumental in the formation of the epidermal barrier by contributing to the terminal differentiation pathway of keratinocytes. This pathway ensures the proper development and maintenance of the skin layers. It associates with proteins such as keratin and filaggrin during this process. Furthermore PADI1 also links to pathways involved in the regulation of intracellular protein modification impacting the overall protein structure and function in tissues.

PADI1 has been linked to psoriasis and skin cancers. In psoriasis the aberrant expression or regulation of PADI1 can lead to improper protein citrullination affecting skin barrier function and immune responses. Moreover dysregulated activity of PADI1 connects to skin cancer progression through its impact on keratinocyte proliferation and differentiation. Research has shown interactions with other proteins like keratin as part of these disease processes relevant to its role in skin pathology.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Catalyzes the deimination of arginine residues of proteins.

Sequence similarities

Belongs to the protein arginine deiminase family.

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Product protocols

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Target data

Catalyzes the deimination of arginine residues of proteins.
See full target information PADI1
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