Recombinant human PADI2 / PAD2 protein is a Human Full Length protein, in the 1 to 665 aa range, expressed in Baculovirus infected Sf9, with >=61% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >=61% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M L R E R T V R L Q Y G S R V E A V Y V L G T Y L W T D V Y S A A P A G A Q T F S L K H S E H V W V E V V R D G E A E E V A T N G K Q R W L L S P S T T L R V T M S Q A S T E A S S D K V T V N Y Y D E E G S I P I D Q A G L F L T A I E I S L D V D A D R D G V V E K N N P K K A S W T W G P E G Q G A I L L V N C D R E T P W L P K E D C R D E K V Y S K E D L K D M S Q M I L R T K G P D R L P A G Y E I V L Y I S M S D S D K V G V F Y V E N P F F G Q R Y I H I L G R R K L Y H V V K Y T G G S A E L L F F V E G L C F P D E G F S G L V S I H V S L L E Y M A Q D I P L T P I F T D T V I F R I A P W I M T P N I L P P V S V F V C C M K D N Y L F L K E V K N L V E K T N C E L K V C F Q Y L N R G D R W I Q D E I E F G Y I E A P H K G F P V V L D S P R D G N L K D F P V K E L L G P D F G Y V T R E P L F E S V T S L D S F G N L E V S P P V T V N G K T Y P L G R I L I G S S F P L S G G R R M T K V V R D F L K A Q Q V Q A P V E L Y S D W L T V G H V D E F M S F V P I P G T K K F L L L M A S T S A C Y K L F R E K Q K D G H G E A I M F K G L G G M S S K R I T I N K I L S N E S L V Q E N L Y F Q R C L D W N R D I L K K E L G L T E Q D I I D L P A L F K M D E D H R A R A F F P N M V N M I V L D K D L G I P K P F G P Q V E E E C C L E M H V R G L L E P L G L E C T F I D D I S A Y H K F L G E V H C G T N V R R K P F T F K W W H M V P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the deimination of arginine residues of proteins.
Alternative names
KIAA0994, PAD2, PDI2, PADI2, Protein-arginine deiminase type-2, PAD-H19, Peptidylarginine deiminase II, Protein-arginine deiminase type II
Recommended products
Recombinant human PADI2 / PAD2 protein is a Human Full Length protein, in the 1 to 665 aa range, expressed in Baculovirus infected Sf9, with >=61% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=61% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- DDDDK tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- ≥19 pmole/min/μg: Enzyme reaction was performed in a buffer (50 mM Tris, pH 7.4, 50 mM NaCl, 10 mM CaCl2, and 2 mM DTT) containing ab196406 and 10 mM BAEE as a substrate for 30 min at 37°C.
- Accession
- Q9Y2J8-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.64% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M L R E R T V R L Q Y G S R V E A V Y V L G T Y L W T D V Y S A A P A G A Q T F S L K H S E H V W V E V V R D G E A E E V A T N G K Q R W L L S P S T T L R V T M S Q A S T E A S S D K V T V N Y Y D E E G S I P I D Q A G L F L T A I E I S L D V D A D R D G V V E K N N P K K A S W T W G P E G Q G A I L L V N C D R E T P W L P K E D C R D E K V Y S K E D L K D M S Q M I L R T K G P D R L P A G Y E I V L Y I S M S D S D K V G V F Y V E N P F F G Q R Y I H I L G R R K L Y H V V K Y T G G S A E L L F F V E G L C F P D E G F S G L V S I H V S L L E Y M A Q D I P L T P I F T D T V I F R I A P W I M T P N I L P P V S V F V C C M K D N Y L F L K E V K N L V E K T N C E L K V C F Q Y L N R G D R W I Q D E I E F G Y I E A P H K G F P V V L D S P R D G N L K D F P V K E L L G P D F G Y V T R E P L F E S V T S L D S F G N L E V S P P V T V N G K T Y P L G R I L I G S S F P L S G G R R M T K V V R D F L K A Q Q V Q A P V E L Y S D W L T V G H V D E F M S F V P I P G T K K F L L L M A S T S A C Y K L F R E K Q K D G H G E A I M F K G L G G M S S K R I T I N K I L S N E S L V Q E N L Y F Q R C L D W N R D I L K K E L G L T E Q D I I D L P A L F K M D E D H R A R A F F P N M V N M I V L D K D L G I P K P F G P Q V E E E C C L E M H V R G L L E P L G L E C T F I D D I S A Y H K F L G E V H C G T N V R R K P F T F K W W H M V P
- Accession
- Q9Y2J8
- Protein length
- Full Length
- Predicted molecular weight
- 77 kDa
- Amino acids
- 1 to 665
- Nature
- Recombinant
- Tags
- DDDDK tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Catalyzes the deimination of arginine residues of proteins.
- Sequence similarities
Belongs to the protein arginine deiminase family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PADI2 also known as PAD2 or PADdeux is a protein that performs post-translational modification by converting arginine residues into citrulline residues in proteins through a process known as citrullination. This protein has an approximate mass of 75 kDa. PADI2 is expressed in many tissues but shows higher levels in the brain skeletal muscle and mammary gland. Within these tissues PADI2 plays a role in regulating various physiological processes by altering protein function through citrullination.
- Biological function summary
PADI2 contributes significantly to the regulation of gene expression protein function and signal transduction. PADI2 does not exist as part of a larger protein complex yet it interacts with other proteins to regulate cellular processes. In particular it modifies histones which impacts chromatin structure and gene expression playing a role in cell differentiation and immune response modulation. Its activity can affect various cellular outcomes by regulating the function and interaction of different protein substrates.
- Pathways
PADI2 takes part in gene regulation and cell differentiation processes by modifying histones and impacting chromatin structure. It is integral in pathways related to inflammation and immune responses such as the NF-kB signaling pathway enhancing its biological role. PADI2 interacts with multiple proteins including histones and other signaling molecules reinforcing its critical role in these pathways.
- Associated diseases and disorders
PADI2 has been linked to rheumatoid arthritis and multiple sclerosis due to its role in immune modulation and inflammation. In rheumatoid arthritis aberrant citrullination by PADI2 contributes to the production of autoantibodies which are involved in the disease's pathogenesis. It also associates with abnormal neural processes in multiple sclerosis where citrullinated proteins potentially contribute to the demyelination process. In these conditions PADI2's function relates closely with other citrullinating proteins such as PADI4 highlighting the interconnectedness of these pathways in disease.
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2 product images
SDS-PAGE - Recombinant human PADI2 / PAD2 protein (ab196406)
4-20% SDS-PAGE analysis of ab196406 at 2.6 μg stained with Coomassie Blue (lane 1). Lane 2 is a protein marker.
Functional Studies - Recombinant human PADI2 / PAD2 protein (ab196406)
Example of specific activity of ab196406
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