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AB196406

Recombinant human PADI2 / PAD2 protein

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Recombinant human PADI2 / PAD2 protein is a Human Full Length protein, in the 1 to 665 aa range, expressed in Baculovirus infected Sf9, with >=61% purity and suitable for SDS-PAGE and Functional studies.The predicted molecular weight of ab196406 protein is 77 kDa.

- Save time and ensure accurate results - use our recombinant Human Peptidyl arginine deiminase 2 (PAD2/ PADI2) protein as a control
- Optimal protein bioactivity and stability

View Alternative Names

KIAA0994, PAD2, PDI2, PADI2, Protein-arginine deiminase type-2, PAD-H19, Peptidylarginine deiminase II, Protein-arginine deiminase type II

2 Images
Functional Studies - Recombinant human PADI2 / PAD2 protein (AB196406)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PADI2 / PAD2 protein (AB196406)

Example of specific activity of ab196406

SDS-PAGE - Recombinant human PADI2 / PAD2 protein (AB196406)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PADI2 / PAD2 protein (AB196406)

4-20% SDS-PAGE analysis of ab196406 at 2.6 μg stained with Coomassie Blue (lane 1). Lane 2 is a protein marker.

Key facts

Purity

>61% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

DDDDK tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

≥19 pmole/min/μg: Enzyme reaction was performed in a buffer (50 mM Tris, pH 7.4, 50 mM NaCl, 10 mM CaCl2, and 2 mM DTT) containing ab196406 and 10 mM BAEE as a substrate for 30 min at 37°C.

Accession

Q9Y2J8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.64% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Ensure the validity of your result using our bioactive recombinant human PADI2 / PAD2 as positive control in SDS-PAGE.


Check out our protein gel staining guide for SDS-PAGE here
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Sequence info

[{"sequence":"MLRERTVRLQYGSRVEAVYVLGTYLWTDVYSAAPAGAQTFSLKHSEHVWVEVVRDGEAEEVATNGKQRWLLSPSTTLRVTMSQASTEASSDKVTVNYYDEEGSIPIDQAGLFLTAIEISLDVDADRDGVVEKNNPKKASWTWGPEGQGAILLVNCDRETPWLPKEDCRDEKVYSKEDLKDMSQMILRTKGPDRLPAGYEIVLYISMSDSDKVGVFYVENPFFGQRYIHILGRRKLYHVVKYTGGSAELLFFVEGLCFPDEGFSGLVSIHVSLLEYMAQDIPLTPIFTDTVIFRIAPWIMTPNILPPVSVFVCCMKDNYLFLKEVKNLVEKTNCELKVCFQYLNRGDRWIQDEIEFGYIEAPHKGFPVVLDSPRDGNLKDFPVKELLGPDFGYVTREPLFESVTSLDSFGNLEVSPPVTVNGKTYPLGRILIGSSFPLSGGRRMTKVVRDFLKAQQVQAPVELYSDWLTVGHVDEFMSFVPIPGTKKFLLLMASTSACYKLFREKQKDGHGEAIMFKGLGGMSSKRITINKILSNESLVQENLYFQRCLDWNRDILKKELGLTEQDIIDLPALFKMDEDHRARAFFPNMVNMIVLDKDLGIPKPFGPQVEEECCLEMHVRGLLEPLGLECTFIDDISAYHKFLGEVHCGTNVRRKPFTFKWWHMVP","proteinLength":"Full Length","predictedMolecularWeight":"77 kDa","actualMolecularWeight":null,"aminoAcidEnd":665,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9Y2J8","tags":[{"tag":"DDDDK","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PADI2 also known as PAD2 or PADdeux is a protein that performs post-translational modification by converting arginine residues into citrulline residues in proteins through a process known as citrullination. This protein has an approximate mass of 75 kDa. PADI2 is expressed in many tissues but shows higher levels in the brain skeletal muscle and mammary gland. Within these tissues PADI2 plays a role in regulating various physiological processes by altering protein function through citrullination.
Biological function summary

PADI2 contributes significantly to the regulation of gene expression protein function and signal transduction. PADI2 does not exist as part of a larger protein complex yet it interacts with other proteins to regulate cellular processes. In particular it modifies histones which impacts chromatin structure and gene expression playing a role in cell differentiation and immune response modulation. Its activity can affect various cellular outcomes by regulating the function and interaction of different protein substrates.

Pathways

PADI2 takes part in gene regulation and cell differentiation processes by modifying histones and impacting chromatin structure. It is integral in pathways related to inflammation and immune responses such as the NF-kB signaling pathway enhancing its biological role. PADI2 interacts with multiple proteins including histones and other signaling molecules reinforcing its critical role in these pathways.

PADI2 has been linked to rheumatoid arthritis and multiple sclerosis due to its role in immune modulation and inflammation. In rheumatoid arthritis aberrant citrullination by PADI2 contributes to the production of autoantibodies which are involved in the disease's pathogenesis. It also associates with abnormal neural processes in multiple sclerosis where citrullinated proteins potentially contribute to the demyelination process. In these conditions PADI2's function relates closely with other citrullinating proteins such as PADI4 highlighting the interconnectedness of these pathways in disease.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Catalyzes the deimination of arginine residues of proteins.

Sequence similarities

Belongs to the protein arginine deiminase family.

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Product protocols

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Target data

Catalyzes the deimination of arginine residues of proteins.
See full target information PADI2
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