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AB196421

Recombinant human PADI3 / PAD3 protein (DDDDK tag C-Terminus)

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Recombinant human PADI3 / PAD3 protein (DDDDK tag C-Terminus) is a Human Full Length protein, in the 1 to 664 aa range, expressed in Baculovirus infected Sf9 cells, with >53%, suitable for SDS-PAGE, FuncS.

View Alternative Names

PAD3, PDI3, PADI3, Protein-arginine deiminase type-3, Peptidylarginine deiminase III, Protein-arginine deiminase type III

2 Images
Functional Studies - Recombinant human PADI3 / PAD3 protein (DDDDK tag C-Terminus) (AB196421)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PADI3 / PAD3 protein (DDDDK tag C-Terminus) (AB196421)

Activity assay of ab196421.

SDS-PAGE - Recombinant human PADI3 / PAD3 protein (DDDDK tag C-Terminus) (AB196421)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PADI3 / PAD3 protein (DDDDK tag C-Terminus) (AB196421)

10% SDS-PAGE analysis of 2.85 μg ab196421 with Coomassie staining.

Key facts

Purity

>53% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

DDDDK tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: ≥22 pmole/min/μg.

Enzyme reaction was performed in a buffer (50 mM Tris, pH 7.4, 50 mM NaCl, 10 mM CaCl2, and 2 mM DTT) containing PADI and 10 mM BAEE as a substrate for 25 min at 37°C.

Accession

Q9ULW8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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Complementary Products

Primary Antibodies

AB172959

Anti-PADI3 / PAD3 antibody [EPR12165(B)]

RabMAb|Recombinant

Immunoprecipitation - Anti-PADI3 / PAD3 antibody [EPR12165(B)] (AB172959)

  • 0
  • 0
Proteins & Peptides

AB313361

Recombinant Human WFDC2 Protein (His tag)

Mass Spectrometry - Recombinant Human WFDC2 Protein (His tag) (AB313361)

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Proteins & Peptides

AB95933

Recombinant human XIAP protein (DDDDK tag N-Terminus)

Functional Studies - Recombinant human XIAP protein (DDDDK tag N-Terminus) (AB95933)

  • 0
  • 0
Proteins & Peptides

AB217407

Recombinant human Vitronectin/S-Protein (Animal Free)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MSLQRIVRVSLEHPTSAVCVAGVETLVDIYGSVPEGTEMFEVYGTPGVDIYISPNMERGRERADTRRWRFDATLEIIVVMNSPSNDLNDSHVQISYHSSHEPLPLAYAVLYLTCVDISLDCDLNCEGRQDRNFVDKRQWVWGPSGYGGILLVNCDRDDPSCDVQDNCDQHVHCLQDLEDMSVMVLRTQGPAALFDDHKLVLHTSSYDAKRAQVFHICGPEDVCEAYRHVLGQDKVSYEVPRLHGDEERFFVEGLSFPDAGFTGLISFHVTLLDDSNEDFSASPIFTDTVVFRVAPWIMTPSTLPPLEVYVCRVRNNTCFVDAVAELARKAGCKLTICPQAENRNDRWIQDEMELGYVQAPHKTLPVVFDSPRNGELQDFPYKRILGPDFGYVTREPRDRSVSGLDSFGNLEVSPPVVANGKEYPLGRILIGGNLPGSSGRRVTQVVRDFLHAQKVQPPVELFVDWLAVGHVDEFLSFVPAPDGKGFRMLLASPGACFKLFQEKQKCGHGRALLFQGVVDDEQVKTISINQVLSNKDLINYNKFVQSCIDWNREVLKRELGLAECDIIDIPQLFKTERKKATAFFPDLVNMLVLGKHLGIPKPFGPIINGCCCLEEKVRSLLEPLGLHCTFIDDFTPYHMLHGEVHCGTNVCRKPFSFKWWNMVP","proteinLength":"Full Length","predictedMolecularWeight":"75 kDa","actualMolecularWeight":null,"aminoAcidEnd":664,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9ULW8","tags":[{"tag":"DDDDK","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PADI3 also known as PAD3 is an enzyme that belongs to the peptidylarginine deiminase family. It converts arginine residues into citrullines in the presence of calcium ions an activity known as citrullination or deimination. PADI3 has a molecular mass of approximately 74 kDa. Expression of this enzyme is found mainly in hair follicles skin and possibly other epithelial tissues where it modifies structural proteins important for tissue organization and function.
Biological function summary

PADI3 plays a role in the formation of the hair shaft by modifying trichohyalin a core structural protein and possibly other substrates within hair matrix keratinocytes. It contributes to the stabilization and structural integrity of hair and skin proteins. Although not massive in function alone PADI3 may work as part of a complex with other proteins to achieve these modifications effectively.

Pathways

PADI3 fits into the intricate web of keratinization and epidermal differentiation processes. It is part of pathways that involve protein deimination alongside proteins such as PAD2 and PAD1 which work together to modify and stabilize intermediate filaments and other structural protein components within the skin and hair.

PADI3 is linked most commonly to hair and skin-related conditions including some forms of alopecia and psoriasis. Studies suggest the enzyme might interact with keratin proteins influencing the abnormal keratinization observed in these disorders. Mutations in PADI3 as well as related proteins like keratin may disrupt normal protein modification and aggregation leading to these pathological conditions.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Catalyzes the deimination of arginine residues of proteins.

Sequence similarities

Belongs to the protein arginine deiminase family.

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Product protocols

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Target data

Catalyzes the deimination of arginine residues of proteins.
See full target information PADI3
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