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AB92772

Recombinant human PAI1 protein

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Recombinant human PAI1 protein is a Human Full Length protein, expressed in Insect cells, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

PAI1, PLANH1, SERPINE1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1

Key facts

Purity

>95% SDS-PAGE

Expression system

Insect cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Kinetic Data: Second order rate constants for inhibition of: uPA = 6.3 X 106 M-1s-1 tPA = 5.7 X 106 M-1s-1 95 (+/-) 5% active by uPA titration

Accession

P05121

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 6.6 Constituents: 0.82% Sodium phosphate, 0.58% Sodium chloride, 0.0292% EDTA

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB116028

Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (AB116028)

  • 5
  • 3
Primary Antibodies

AB66705

Anti-PAI1 antibody

BOND RX™ Validated

Western blot - Anti-PAI1 antibody (AB66705)

  • 4
  • 8
Assay Kits

AB242296

Gamma H2A.X Staining Kit

Fluorescence Microscopy - Gamma H2A.X Staining Kit (AB242296)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Proteins & Peptides

AB217407

Recombinant human Vitronectin/S-Protein (Animal Free)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Solubility: > 2 mg/mL and < 5 mg/mL

The stable mutant contains mutations to confer additional stability to the otherwise labile molecule.

Produced using non-baculovirus insect cells.

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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P05121","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
True
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Specifications

Form

Liquid

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General info

Function

Serine protease inhibitor. Inhibits TMPRSS7 (PubMed : 15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed : 17912461, PubMed : 8481516, PubMed : 9207454, PubMed : 21925150). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed : 9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed : 15001579, PubMed : 9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed : 18386027). It is involved in cellular and replicative senescence (PubMed : 16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed : 25808697, PubMed : 27046084).

Sequence similarities

Belongs to the serpin family.

Post-translational modifications

Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond.

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Product protocols

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Target data

Serine protease inhibitor. Inhibits TMPRSS7 (PubMed : 15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed : 17912461, PubMed : 8481516, PubMed : 9207454, PubMed : 21925150). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed : 9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed : 15001579, PubMed : 9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed : 18386027). It is involved in cellular and replicative senescence (PubMed : 16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed : 25808697, PubMed : 27046084).
See full target information SERPINE1
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