Recombinant Human PAK2 protein

Specifications

Form

Liquid

Additional notes

Assessed by densitometry. Affinity purified.

General info

Function

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation (PubMed : 12853446, PubMed : 16617111, PubMed : 19273597, PubMed : 19923322, PubMed : 33693784, PubMed : 7744004, PubMed : 9171063). Acts as a downstream effector of the small GTPases CDC42 and RAC1 (PubMed : 7744004). Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues (PubMed : 7744004). Full-length PAK2 stimulates cell survival and cell growth (PubMed : 7744004). Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration (PubMed : 21317288). Phosphorylates JUN and plays an important role in EGF-induced cell proliferation (PubMed : 21177766). Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP (PubMed : 21724829). Phosphorylates CASP7, thereby preventing its activity (PubMed : 21555521, PubMed : 27889207). Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis (PubMed : 19273597, PubMed : 19923322). On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway (PubMed : 12853446, PubMed : 16617111, PubMed : 9171063). Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation (PubMed : 15234964).

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Post-translational modifications

Full-length PAK2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.. During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.. Ubiquitinated, leading to its proteasomal degradation.. PAK-2p34. PAK-2p34 is myristoylated.

Subcellular localisation

Nucleus