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AB89757

Recombinant human PAK6 protein

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Recombinant human PAK6 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

PAK5, PAK6, Serine/threonine-protein kinase PAK 6, PAK-5, p21-activated kinase 6, PAK-6

4 Images
Functional Studies - Recombinant human PAK6 protein (AB89757)
  • FuncS

Unknown

Functional Studies - Recombinant human PAK6 protein (AB89757)

Kinase Assay demonstrating specific activity of ab89757.

Functional Studies - Recombinant human PAK6 protein (AB89757)
  • FuncS

Unknown

Functional Studies - Recombinant human PAK6 protein (AB89757)

The specific activity of PAK6 (ab89757) was determined to be 116 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant human PAK6 protein (AB89757)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PAK6 protein (AB89757)

SDS-PAGE showing ab89757 at approximately 110kDa.

SDS-PAGE - Recombinant human PAK6 protein (AB89757)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PAK6 protein (AB89757)

SDS PAGE analysis of ab89757

Key facts

Purity

>85% Densitometry

Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS, WB

applications

Biologically active

Yes

Biological activity

The Specific activity of ab89757 was determined to be 137 nmol/min/mg.

Accession

Q9NQU5

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Complementary Products

Primary Antibodies

AB41431

Anti-PAK6 antibody

Western blot - Anti-PAK6 antibody (AB41431)

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Proteins & Peptides

AB177262

Recombinant human WNK1 protein (Tagged)

Functional Studies - Recombinant human WNK1 protein (Tagged) (AB177262)

  • 0
  • 0
Proteins & Peptides

AB101548

Recombinant human ULK3 protein

Functional Studies - Recombinant human ULK3 protein (AB101548)

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  • 0
Proteins & Peptides

AB125622

Recombinant human ULK2 protein

Functional Studies - Recombinant human ULK2 protein (AB125622)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab204872 (PAK peptide substrate) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9NQU5","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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General info

Function

Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Also inhibits ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Post-translational modifications

Autophosphorylated. Phosphorylated by MAP2K6//MAPKK6, leading to PAK6 activation.

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Product protocols

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Target data

Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Also inhibits ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD.
See full target information PAK6
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Product promise

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