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AB157887

Recombinant Human Pancreatic alpha amylase protein (GST tag N-Terminus)

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Recombinant Human Pancreatic alpha amylase protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 511 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

Pancreatic alpha-amylase, PA, AMY2A

1 Images
SDS-PAGE - Recombinant Human Pancreatic alpha amylase protein (GST tag N-Terminus) (AB157887)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Pancreatic alpha amylase protein (GST tag N-Terminus) (AB157887)

ab157887 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

P04746

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Assay Kits

AB137969

Human Pancreatic Amylase ELISA Kit

Sandwich ELISA - Human Pancreatic Amylase ELISA Kit (AB137969)

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Proteins & Peptides

AB276562

Recombinant Human AMY2B protein (His tag)

SDS-PAGE - Recombinant Human AMY2B protein (His tag) (AB276562)

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Assay Kits

AB102523

Amylase Assay Kit (Colorimetric)

Functional Studies - Amylase Assay Kit (Colorimetric) (AB102523)

  • 5
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Primary Antibodies

AB199132

Anti-Pancreatic alpha amylase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic alpha amylase antibody (AB199132)

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  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MKFFLLLFTIGFCWAQYSPNTQQGRTSIVHLFEWRWVDIALECERYLAPKGFGGVQVSPPNENVAIYNPFRPWWERYQPVSYKLCTRSGNEDEFRNMVTRCNNVGVRIYVDAVINHMCGNAVSAGTSSTCGSYFNPGSRDFPAVPYSGWDFNDGKCKTGSGDIENYNDATQVRDCRLTGLLDLALEKDYVRSKIAEYMNHLIDIGVAGFRLDASKHMWPGDIKAILDKLHNLNSNWFPAGSKPFIYQEVIDLGGEPIKSSDYFGNGRVTEFKYGAKLGTVIRKWNGEKMSYLKNWGEGWGFVPSDRALVFVDNHDNQRGHGAGGASILTFWDARLYKMAVGFMLAHPYGFTRVMSSYRWPRQFQNGNDVNDWVGPPNNNGVIKEVTINPDTTCGNDWVCEHRWRQIRNMVIFRNVVDGQPFTNWYDNGSNQVAFGRGNRGFIVFNNDDWSFSLTLQTGLPAGTYCDVISGDKINGNCTGIKIYVSDDGKAHFSISNSAEDPFIAIHAESKL","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":511,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P04746","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pancreatic alpha amylase also known as AMY2A is an enzyme responsible for catalyzing the hydrolysis of starch into sugars. This enzyme is an important amylase protein with a molecular mass of approximately 56 kDa. It is abundantly expressed in the pancreas of mammals playing a significant role in the digestive process. The product of its activity maltose contributes to carbohydrate digestion allowing for energy production in the body.
Biological function summary

Pancreatic alpha amylase assists in the breakdown of large polysaccharides into smaller maltose units. This enzyme can act independently and does not require a complex to function effectively. It carries out its activity primarily in the small intestine where it continues the digestive process initiated by salivary alpha amylase. This amylase protein ensures that starches consumed in the diet are converted into forms absorbable by the body contributing to overall metabolism.

Pathways

Pancreatic alpha amylase is a critical enzyme in the digestive pathway especially in the carbohydrate digestion and absorption pathway. Its activity complements that of other amylase enzymes including salivary alpha amylase to ensure efficient starch breakdown. Additionally pancreatic alpha amylase works in conjunction with proteins like glucose transporters which facilitate the entry of glucose into cells after starch breakdown.

The activity of pancreatic alpha amylase often links to conditions such as pancreatitis and type 2 diabetes. Elevated levels of this enzyme in the bloodstream can indicate acute pancreatitis highlighting its significance in diagnosing this disorder. In the context of diabetes altered pancreatic amylase activity can affect glucose metabolism. The enzyme's association with glucose-regulating proteins such as insulin makes it a focal point in understanding diabetic pathophysiology.
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Specifications

Form

Liquid

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General info

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

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Product protocols

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Target data

See full target information Pancreatic alpha-amylase
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