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AB276560

Recombinant Human Pancreatic Lipase/PTL protein (His tag)

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Recombinant Human Pancreatic Lipase/PTL protein (His tag) is a Human Full Length protein, in the 1 to 465 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

Pancreatic triacylglycerol lipase, PL, PTL, Pancreatic lipase, PNLIP

1 Images
SDS-PAGE - Recombinant Human Pancreatic Lipase/PTL protein (His tag) (AB276560)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Pancreatic Lipase/PTL protein (His tag) (AB276560)

SDS-PAGE analysis of ab276560

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P16233

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MLPLWTLSLLLGAVAGKEVCYERLGCFSDDSPWSGITERPLHILPWSPKDVNTRFLLYTNENPNNFQEVAADSSSISGSNFKTNRKTRFIIHGFIDKGEENWLANVCKNLFKVESVNCICVDWKGGSRTGYTQASQNIRIVGAEVAYFVEFLQSAFGYSPSNVHVIGHSLGAHAAGEAGRRTNGTIGRITGLDPAEPCFQGTPELVRLDPSDAKFVDVIHTDGAPIVPNLGFGMSQVVGHLDFFPNGGVEMPGCKKNILSQIVDIDGIWEGTRDFAACNHLRSYKYYTDSIVNPDGFAGFPCASYNVFTANKCFPCPSGGCPQMGHYADRYPGKTNDVGQKFYLDTGDASNFARWRYKVSVTLSGKKVTGHILVSLFGNKGNSKQYEIFKGTLKPDSTHSNEFDSDVDVGDLQMVKFIWYNNVINPTLPRVGASKIIVETNVGKQFNFCSPETVREEVLLTLTPC","proteinLength":"Full Length","predictedMolecularWeight":"51 kDa","actualMolecularWeight":null,"aminoAcidEnd":465,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P16233","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pancreatic Lipase also known as PTL or Lipase Pancreas is an enzyme with a molecular mass around 3000 daltons. It is important for the digestion of dietary fats and is expressed in the pancreas of several animals including the mouse and rat. The enzyme hydrolyzes triglycerides into monoglycerides and free fatty acids facilitating the absorption of fat-soluble vitamins. The presence of pancreatic lipase in rodent models like 'pancreas in rat' or 'mouse pancreas' aids in studying its mechanical function which is significant for understanding lipid metabolism.
Biological function summary

Pancreatic lipase plays a central role in the digestive system converting dietary fats into absorbable units. Although primarily not part of any complex its activity depends on the presence of bile salts and co-lipase. Co-lipase binds to pancreatic lipase and enhances its efficiency by stabilizing the enzyme-substrate complex in the presence of bile supporting lipid digestion and absorption.

Pathways

The enzyme is an essential component of the lipid digestion and absorption pathways. It operates closely with other lipases like gastric lipase sequentially breaking down fats from different stages of digestion. The pathways involve the secretion of bile from the liver which emulsifies fats enabling pancreatic lipase to act more efficiently. As part of the larger digestion process the enzyme plays a role in maintaining metabolic homeostasis by interlinking with hormones like insulin which further regulates blood glucose levels based on energy intake from fats.

Pancreatic lipase has significant implications in conditions like hyperlipidemia and pancreatitis. Defects or deficiencies in pancreatic lipase activity can result in malabsorption syndromes affecting nutritional status and leading to weight loss and vitamin deficiencies. Chronic pancreatitis an inflammatory disease with progressive exocrine pancreatic insufficiency involves diminished pancreatic lipase function often requiring enzyme supplementation as part of the treatment. The connection of pancreatic lipase to hyperlipidemia relates to its interaction with proteins like lipoprotein lipase which manages lipid levels within the bloodstream.
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Specifications

Form

Lyophilized

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General info

Function

Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones.

Sequence similarities

Belongs to the AB hydrolase superfamily. Lipase family.

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Product protocols

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Target data

Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones.
See full target information PNLIP
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