Recombinant Human Pancreatic Lipase/PTL protein (His tag) is a Human Full Length protein, in the 1 to 465 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M L P L W T L S L L L G A V A G K E V C Y E R L G C F S D D S P W S G I T E R P L H I L P W S P K D V N T R F L L Y T N E N P N N F Q E V A A D S S S I S G S N F K T N R K T R F I I H G F I D K G E E N W L A N V C K N L F K V E S V N C I C V D W K G G S R T G Y T Q A S Q N I R I V G A E V A Y F V E F L Q S A F G Y S P S N V H V I G H S L G A H A A G E A G R R T N G T I G R I T G L D P A E P C F Q G T P E L V R L D P S D A K F V D V I H T D G A P I V P N L G F G M S Q V V G H L D F F P N G G V E M P G C K K N I L S Q I V D I D G I W E G T R D F A A C N H L R S Y K Y Y T D S I V N P D G F A G F P C A S Y N V F T A N K C F P C P S G G C P Q M G H Y A D R Y P G K T N D V G Q K F Y L D T G D A S N F A R W R Y K V S V T L S G K K V T G H I L V S L F G N K G N S K Q Y E I F K G T L K P D S T H S N E F D S D V D V G D L Q M V K F I W Y N N V I N P T L P R V G A S K I I V E T N V G K Q F N F C S P E T V R E E V L L T L T P C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones.
Alternative names
Pancreatic triacylglycerol lipase, PL, PTL, Pancreatic lipase, PNLIP
Recommended products
Recombinant Human Pancreatic Lipase/PTL protein (His tag) is a Human Full Length protein, in the 1 to 465 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P16233-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M L P L W T L S L L L G A V A G K E V C Y E R L G C F S D D S P W S G I T E R P L H I L P W S P K D V N T R F L L Y T N E N P N N F Q E V A A D S S S I S G S N F K T N R K T R F I I H G F I D K G E E N W L A N V C K N L F K V E S V N C I C V D W K G G S R T G Y T Q A S Q N I R I V G A E V A Y F V E F L Q S A F G Y S P S N V H V I G H S L G A H A A G E A G R R T N G T I G R I T G L D P A E P C F Q G T P E L V R L D P S D A K F V D V I H T D G A P I V P N L G F G M S Q V V G H L D F F P N G G V E M P G C K K N I L S Q I V D I D G I W E G T R D F A A C N H L R S Y K Y Y T D S I V N P D G F A G F P C A S Y N V F T A N K C F P C P S G G C P Q M G H Y A D R Y P G K T N D V G Q K F Y L D T G D A S N F A R W R Y K V S V T L S G K K V T G H I L V S L F G N K G N S K Q Y E I F K G T L K P D S T H S N E F D S D V D V G D L Q M V K F I W Y N N V I N P T L P R V G A S K I I V E T N V G K Q F N F C S P E T V R E E V L L T L T P C
- Accession
- P16233
- Protein length
- Full Length
- Predicted molecular weight
- 51 kDa
- Amino acids
- 1 to 465
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones.
- Sequence similarities
Belongs to the AB hydrolase superfamily. Lipase family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pancreatic Lipase also known as PTL or Lipase Pancreas is an enzyme with a molecular mass around 3000 daltons. It is important for the digestion of dietary fats and is expressed in the pancreas of several animals including the mouse and rat. The enzyme hydrolyzes triglycerides into monoglycerides and free fatty acids facilitating the absorption of fat-soluble vitamins. The presence of pancreatic lipase in rodent models like 'pancreas in rat' or 'mouse pancreas' aids in studying its mechanical function which is significant for understanding lipid metabolism.
- Biological function summary
Pancreatic lipase plays a central role in the digestive system converting dietary fats into absorbable units. Although primarily not part of any complex its activity depends on the presence of bile salts and co-lipase. Co-lipase binds to pancreatic lipase and enhances its efficiency by stabilizing the enzyme-substrate complex in the presence of bile supporting lipid digestion and absorption.
- Pathways
The enzyme is an essential component of the lipid digestion and absorption pathways. It operates closely with other lipases like gastric lipase sequentially breaking down fats from different stages of digestion. The pathways involve the secretion of bile from the liver which emulsifies fats enabling pancreatic lipase to act more efficiently. As part of the larger digestion process the enzyme plays a role in maintaining metabolic homeostasis by interlinking with hormones like insulin which further regulates blood glucose levels based on energy intake from fats.
- Associated diseases and disorders
Pancreatic lipase has significant implications in conditions like hyperlipidemia and pancreatitis. Defects or deficiencies in pancreatic lipase activity can result in malabsorption syndromes affecting nutritional status and leading to weight loss and vitamin deficiencies. Chronic pancreatitis an inflammatory disease with progressive exocrine pancreatic insufficiency involves diminished pancreatic lipase function often requiring enzyme supplementation as part of the treatment. The connection of pancreatic lipase to hyperlipidemia relates to its interaction with proteins like lipoprotein lipase which manages lipid levels within the bloodstream.
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SDS-PAGE - Recombinant Human Pancreatic Lipase/PTL protein (His tag) (ab276560)
SDS-PAGE analysis of ab276560
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