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AB236931

Recombinant Human papillomavirus HPV18 E7 protein (His tag)

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Recombinant Human papillomavirus HPV18 E7 protein (His tag) is a Human papillomavirus type 18 Full Length protein, in the 1 to 105 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Protein E7, E7

3 Images
Mass Spectrometry - Recombinant Human papillomavirus HPV18 E7 protein (His tag) (AB236931)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human papillomavirus HPV18 E7 protein (His tag) (AB236931)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab236931 could indicate that this peptide derived from E.coli-expressed Human papillomavirus HPV18 E7.

Mass Spectrometry - Recombinant Human papillomavirus HPV18 E7 protein (His tag) (AB236931)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human papillomavirus HPV18 E7 protein (His tag) (AB236931)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab236931 could indicate that this peptide derived from E.coli-expressed Human papillomavirus HPV18 E7.

SDS-PAGE - Recombinant Human papillomavirus HPV18 E7 protein (His tag) (AB236931)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human papillomavirus HPV18 E7 protein (His tag) (AB236931)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel analysis of ab236931.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P06788

Animal free

No

Carrier free

No

Species

Human papillomavirus type 18

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MHGPKATLQDIVLHLEPQNEIPVDLLCHEQLSDSEEENDEIDGVNHQHLPARRAEPQRHTMLCMCCKCEARIKLVVESSADDLRAFQQLFLNTLSFVCPWCASQQ","proteinLength":"Full Length","predictedMolecularWeight":"16 kDa","actualMolecularWeight":null,"aminoAcidEnd":105,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P06788","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Also plays a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta) (PubMed : 26405230).

Sequence similarities

Belongs to the papillomaviridae E7 protein family.

Post-translational modifications

Highly phosphorylated.

Subcellular localisation

Host nucleus

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Product protocols

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Target data

Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Also plays a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta) (PubMed : 26405230).
See full target information E7
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