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Recombinant Human PAPPA Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293, with >=80% purity and suitable for sELISA, SDS-PAGE.

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Images

SDS-PAGE - Recombinant Human PAPPA Protein Standard (His tag) (AB316639), expandable thumbnail
  • Sandwich ELISA - Recombinant Human PAPPA Protein Standard (His tag) (AB316639), expandable thumbnail

Key facts

Purity

>=80% SDS-PAGE

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

sELISA, SDS-PAGE

Biologically active

No

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.

Alternative names

Recombinant Human PAPPA Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293, with >=80% purity and suitable for sELISA, SDS-PAGE.

Key facts

Purity

>=80% SDS-PAGE

Expression system

HEK 293 cells

Applications

sELISA, SDS-PAGE

Accession
Q13219-1
Animal free

Yes

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

Sequence info

Amino acid sequence

Accession

Q13219

Protein length

Fragment

Predicted molecular weight

174.2 kDa

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Liquid

General info

Function

Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.

Sequence similarities

Belongs to the peptidase M43B family.

Post-translational modifications

There appear to be no free sulfhydryl groups.

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • SDS-PAGE - Recombinant Human PAPPA Protein Standard (His tag) (ab316639), expandable thumbnail

    SDS-PAGE - Recombinant Human PAPPA Protein Standard (His tag) (ab316639)

    SDS-PAGE analysis of ab316639 under reducing conditions for 2ug protein.

  • Sandwich ELISA - Recombinant Human PAPPA Protein Standard (His tag) (ab316639), expandable thumbnail

    Sandwich ELISA - Recombinant Human PAPPA Protein Standard (His tag) (ab316639)

    Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com