Recombinant Human PAPPA Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293, with >=80% purity and suitable for sELISA, SDS-PAGE.
>=80% SDS-PAGE
HEK 293 cells
His tag C-Terminus
sELISA, SDS-PAGE
No
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.
Pappalysin-1, Insulin-like growth factor-dependent IGF-binding protein 4 protease, Pregnancy-associated plasma protein A, IGF-dependent IGFBP-4 protease, IGFBP-4ase, PAPP-A, PAPPA
Recombinant Human PAPPA Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293, with >=80% purity and suitable for sELISA, SDS-PAGE.
>=80% SDS-PAGE
HEK 293 cells
His tag C-Terminus
sELISA, SDS-PAGE
No
Yes
Human
pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic
Fragment
174.2 kDa
Recombinant
His tag C-Terminus
Liquid
Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.
Belongs to the peptidase M43B family.
There appear to be no free sulfhydryl groups.
Dry Ice
-80°C
-80°C
Upon delivery aliquot
Avoid freeze / thaw cycle
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
SDS-PAGE analysis of ab316639 under reducing conditions for 2ug protein.
Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
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