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Recombinant Human PAR1/Thrombin Receptor Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, sELISA.

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Images

Sandwich ELISA - Recombinant Human PAR1/Thrombin Receptor Protein Standard (His tag) (AB316672), expandable thumbnail
  • SDS-PAGE - Recombinant Human PAR1/Thrombin Receptor Protein Standard (His tag) (AB316672), expandable thumbnail

Key facts

Purity

>=80% SDS-PAGE

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, sELISA

Biologically active

No

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

High affinity receptor that binds the activated thrombin, leading to calcium release from intracellular stores (PubMed:1672265, PubMed:8136362). The thrombin-activated receptor signaling pathway is mediated through PTX-insensitive G proteins, activation of phospholipase C resulting in the production of 1D-myo-inositol 1,4,5-trisphosphate (InsP3) which binds to InsP3 receptors causing calcium release from the stores (By similarity). In astrocytes, the calcium released into the cytosol allows the Ca(2+)-dependent release of L-glutamate into the synaptic cleft through BEST1, that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation (By similarity). May play a role in platelets activation and in vascular development (PubMed:10079109). Mediates up-regulation of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, triggered by coagulation factor Xa (F10) in cardiac fibroblasts and umbilical vein endothelial cells (PubMed:30568593, PubMed:34831181).

Alternative names

Recombinant Human PAR1/Thrombin Receptor Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, sELISA.

Key facts

Purity

>=80% SDS-PAGE

Expression system

HEK 293 cells

Applications

SDS-PAGE, sELISA

Accession
P25116-1
Animal free

Yes

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

Sequence info

Amino acid sequence

Accession

P25116

Protein length

Fragment

Predicted molecular weight

9.7 kDa

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Liquid

General info

Function

High affinity receptor that binds the activated thrombin, leading to calcium release from intracellular stores (PubMed:1672265, PubMed:8136362). The thrombin-activated receptor signaling pathway is mediated through PTX-insensitive G proteins, activation of phospholipase C resulting in the production of 1D-myo-inositol 1,4,5-trisphosphate (InsP3) which binds to InsP3 receptors causing calcium release from the stores (By similarity). In astrocytes, the calcium released into the cytosol allows the Ca(2+)-dependent release of L-glutamate into the synaptic cleft through BEST1, that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation (By similarity). May play a role in platelets activation and in vascular development (PubMed:10079109). Mediates up-regulation of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, triggered by coagulation factor Xa (F10) in cardiac fibroblasts and umbilical vein endothelial cells (PubMed:30568593, PubMed:34831181).

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Post-translational modifications

Proteolytic cleavage by thrombin generates a new N-terminus that functions as a tethered ligand (PubMed:1672265, PubMed:7744748). Also proteolytically cleaved by cathepsin CTSG (PubMed:7744748). Cleavage at 41-Arg-|-Ser-42 by CTSG results in receptor activation while cleavage at 55-Phe-|-Trp-56 results in inhibition of receptor activation (PubMed:7744748).

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Recombinant Human PAR1/Thrombin Receptor Protein Standard (His tag) (ab316672), expandable thumbnail

    Sandwich ELISA - Recombinant Human PAR1/Thrombin Receptor Protein Standard (His tag) (ab316672)

    Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

  • SDS-PAGE - Recombinant Human PAR1/Thrombin Receptor Protein Standard (His tag) (ab316672), expandable thumbnail

    SDS-PAGE - Recombinant Human PAR1/Thrombin Receptor Protein Standard (His tag) (ab316672)

    SDS-PAGE analysis of ab316672 under reducing conditions for 2ug protein.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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