Recombinant Human Parathyroid Hormone protein is a Human Full Length protein, in the 32 to 115 aa range, expressed in Escherichia coli, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, WB, ELISA.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, ELISA
- Biologically active
- No
Amino acid sequence
M S V S E I Q L M H N L G K H L N S M E R V E W L R K K L Q D V H N F V A L G A P L A P R D A G S Q R P R K K E D N V L V E S H E K S L G E A D K A D V N V L T K A K S Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab51234 can be used as a WB positive control in conjunction with Anti-Parathyroid Hormone antibody ab40630. |
Application ELISA | Reactivity Reacts | Dilution info - | Notes Dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [A1/70] to Parathyroid Hormone (Anti-Parathyroid Hormone antibody [A1/70] ab14493) at 0.2μg/ml and Detector Antibody Rabbit polyclonal to Parathyroid Hormone (ab53040) at 0.5μg/ml. |
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Target data
Function
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
Alternative names
Parathyroid hormone, PTH, Parathormone, Parathyrin
Recommended products
Recombinant Human Parathyroid Hormone protein is a Human Full Length protein, in the 32 to 115 aa range, expressed in Escherichia coli, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, WB, ELISA.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, ELISA
- Biologically active
- No
- Accession
- P01270-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 0.0584% EDTA
Sequence info
Amino acid sequence
- M S V S E I Q L M H N L G K H L N S M E R V E W L R K K L Q D V H N F V A L G A P L A P R D A G S Q R P R K K E D N V L V E S H E K S L G E A D K A D V N V L T K A K S Q
- Accession
- P01270
- Protein length
- Full Length
- Predicted molecular weight
- 9.55 kDa
- Amino acids
- 32 to 115
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Human Parathyroid Hormone(amino acids 32-115) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques.
General info
- Function
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
- Sequence similarities
Belongs to the parathyroid hormone family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Parathyroid hormone (PTH) also known as the parathyrin is a hormone protein with a molecular mass of about 9.4 kDa. It is synthesized and secreted by the chief cells of the parathyroid glands. PTH plays an important role in regulating calcium levels in the blood. Its molecular action involves binding to PTH receptors which are mainly located in bones and kidneys to increase calcium concentration by promoting its release from bones and reabsorption in the kidneys. This hormone is also present in the bloodstream as a parathyroid hormone peptide.
- Biological function summary
PTH regulates calcium and phosphate metabolism in the body. It does not function as part of a larger complex but acts independently to control these important minerals. The hormone affects osteoclast activity in bones enhancing calcium and phosphate release. Additionally in the kidneys it reduces phosphate reabsorption while increasing calcium reabsorption. This balance maintained by PTH is essential for bone health and metabolic functions often associated with parathyroid hormone-related proteins in its activity.
- Pathways
PTH is integral to the calcium signaling pathway and the renal phosphate reabsorption pathway. PTH works in concert with vitamin D to enhance intestinal calcium absorption. Calcitriol the active form of vitamin D depends on PTH for its synthesis in the kidneys linking the two in calcium homeostasis. Through these pathways PTH also interacts with the calcitonin hormone to moderate calcium levels creating a balance between mineral deposition and resorption in bones.
- Associated diseases and disorders
PTH is closely associated with hyperparathyroidism and hypoparathyroidism known as pan hypoparathyroidism when complete underactivity of the parathyroid glands occurs. In hyperparathyroidism an overproduction of PTH leads to high blood calcium levels and associated symptoms. Conversely hypoparathyroidism results in low calcium levels due to insufficient PTH secretion. Both conditions affect calcium metabolism and relate to proteins like calcium-sensing receptor and PTH-related peptide highlighting how critical PTH's function is in endocrine and metabolic health.
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3 product images
Sandwich ELISA - Recombinant Human Parathyroid Hormone protein (ab51234)
Standard curve for Parathyroid Hormone (Analyte: ab51234); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [A1/70] to Parathyroid Hormone (Anti-Parathyroid Hormone antibody [A1/70] ab14493) at 0.2μg/ml and Detector Antibody Rabbit polyclonal to Parathyroid Hormone (ab53040) at 0.5μg/ml.
SDS-PAGE - Recombinant Human Parathyroid Hormone protein (ab51234)
ab51234 on 15%SDS-PAGE
Western blot - Recombinant Human Parathyroid Hormone protein (ab51234)
All lanes: Western blot - Anti-Parathyroid Hormone antibody (Anti-Parathyroid Hormone antibody ab40630) at 1 µg/mL
All lanes: Western blot - Recombinant Human Parathyroid Hormone protein (ab51234) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 13 kDa
Exposure time: 4min
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