Recombinant Human Parathyroid Hormone protein (Tag Free)
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(1 Publication)
Recombinant Human Parathyroid Hormone protein (Tag Free) is a Human Full Length protein, in the 32 to 115 aa range, expressed in Escherichia coli, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, WB, ELISA.
View Alternative Names
Parathyroid hormone, PTH, Parathormone, Parathyrin
- sELISA
Unknown
Sandwich ELISA - Recombinant Human Parathyroid Hormone protein (AB51234)
Standard curve for Parathyroid Hormone (Analyte : ab51234); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [A1/70] to Parathyroid Hormone (ab14493) at 0.2μg/ml and Detector Antibody Rabbit polyclonal to Parathyroid Hormone (ab53040) at 0.5μg/ml.
- WB
Unknown
Western blot - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)
All lanes:
Western blot - Anti-Parathyroid Hormone antibody (<a href='/en-us/products/primary-antibodies/parathyroid-hormone-antibody-ab40630'>ab40630</a>) at 1 µg/mL
All lanes:
Western blot - Recombinant Human Parathyroid Hormone protein (Tag Free) (ab51234) at 0.01 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution
Predicted band size: 13 kDa
true
Exposure time: 4min
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)
ab51234 on 15%SDS-PAGE
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PTH regulates calcium and phosphate metabolism in the body. It does not function as part of a larger complex but acts independently to control these important minerals. The hormone affects osteoclast activity in bones enhancing calcium and phosphate release. Additionally in the kidneys it reduces phosphate reabsorption while increasing calcium reabsorption. This balance maintained by PTH is essential for bone health and metabolic functions often associated with parathyroid hormone-related proteins in its activity.
Pathways
PTH is integral to the calcium signaling pathway and the renal phosphate reabsorption pathway. PTH works in concert with vitamin D to enhance intestinal calcium absorption. Calcitriol the active form of vitamin D depends on PTH for its synthesis in the kidneys linking the two in calcium homeostasis. Through these pathways PTH also interacts with the calcitonin hormone to moderate calcium levels creating a balance between mineral deposition and resorption in bones.
Specifications
Form
Liquid
Additional notes
Human Parathyroid Hormone(amino acids 32-115) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques.
General info
Function
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
Sequence similarities
Belongs to the parathyroid hormone family.
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Microbial cell factories 23:273 PubMed39390566
2024
Applications
Unspecified application
Species
Unspecified reactive species
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