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AB79742

Recombinant human PARP11 protein (GST N-Terminus + 6x His tag C-Terminus)

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Recombinant human PARP11 protein (GST N-Terminus + 6x His tag C-Terminus) is a Human Fragment protein, in the 8 to 338 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

C12orf6, PARP11, Protein mono-ADP-ribosyltransferase PARP11, ADP-ribosyltransferase diphtheria toxin-like 11, Poly [ADP-ribose] polymerase 11, ARTD11, PARP-11

3 Images
Functional Studies - Recombinant human PARP11 protein (GST N-Terminus + 6x His tag C-Terminus) (AB79742)
  • FuncS

Unknown

Functional Studies - Recombinant human PARP11 protein (GST N-Terminus + 6x His tag C-Terminus) (AB79742)

Image showing specific activity of ab79742.

Western blot - Recombinant human PARP11 protein (GST N-Terminus + 6x His tag C-Terminus) (AB79742)
  • WB

Unknown

Western blot - Recombinant human PARP11 protein (GST N-Terminus + 6x His tag C-Terminus) (AB79742)

All lanes:

Anti-PARP11 antibody (<a href='/en-us/products/unavailable/parp11-antibody-ab84935'>ab84935</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant human PARP11 protein (GST N-Terminus + 6x His tag C-Terminus) (ab79742) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 12min

SDS-PAGE - Recombinant human PARP11 protein (GST N-Terminus + 6x His tag C-Terminus) (AB79742)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PARP11 protein (GST N-Terminus + 6x His tag C-Terminus) (AB79742)

10% SDS-PAGE showing ab79742 at approximately 65.6kDa (4μg).

Key facts

Purity

>80% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus 6x His tag C-Terminus

Applications

FuncS, SDS-PAGE, WB

applications

Biologically active

Yes

Biological activity

Specific Activity: 23 U/mg.

Accession

Q9NR21

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Complementary Products

Proteins & Peptides

AB79663

Recombinant human PARP1 protein (GST tag N-Terminus)

Functional Studies - Recombinant human PARP1 protein (GST tag N-Terminus) (AB79663)

  • 0
  • 0
Primary Antibodies

AB237411

Alexa Fluor® 488 Anti-PARP1 antibody [E102]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-PARP1 antibody [E102] (AB237411)

  • 0
  • 0
Primary Antibodies

AB241967

Anti-PARP12 antibody

Immunoprecipitation - Anti-PARP12 antibody (AB241967)

  • 0
  • 0
Primary Antibodies

AB3534

Anti-Pseudorabies Virus antibody

Immunohistochemistry (Frozen sections) - Anti-Pseudorabies Virus antibody (AB3534)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab79742 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/parp11-antibody-ab84935'>ab84935</a>.</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Ribosylation reaction is conducted in wells coated with the mixture of histones at room temperature for 1 hour in 50 mM Tris-HCl (pH8.0), 5 mM MgCl<sub>2</sub> and 20 uM ZnCl<sub>2</sub> assay buffer. One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into acid-insoluble form.</p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":338,"aminoAcidStart":8,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9NR21","tags":[{"tag":"GST","terminus":"N-Terminus"},{"tag":"6x His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PARP11 also known as Poly (ADP-ribose) Polymerase 11 is an enzyme involved in regulating protein function through mono-ADP-ribosylation a post-translational modification. The protein has a molecular mass of about 40 kDa. PARP11 is expressed in various tissues notably in the liver and immune system cells. This enzyme plays a role in establishing cellular processes linked to protein stability and signaling.
Biological function summary

PARP11 modifies proteins by adding ADP-ribose units impacting their interaction and activity. It is not part of a large complex but works on its own. Its activity influences cellular responses to stress and can alter how cells communicate and survive under adverse conditions. These functions highlight its significance in controlling immune responses and inflammation.

Pathways

PARP11 contributes to the regulation of the NF-kB signaling and interferon pathways. These pathways are essential for immune function and inflammation control. PARP11 interacts with other proteins like PARP1 in the NF-kB pathway impacting transcriptional activity and inflammatory responses. Its actions help modulate cellular responses to DNA damage and stress signals.

PARP11 has been connected to cancer and autoimmune diseases. Changes in its activity can influence tumor growth and immune responses making it an attractive target in cancer and autoimmune disease research. Its interaction with proteins like PARP1 often contributes to these pathological conditions suggesting that targeting PARP11 might offer therapeutic benefits in managing these diseases.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins (PubMed : 25043379, PubMed : 25673562). Plays a role in nuclear envelope stability and nuclear remodeling during spermiogenesis (By similarity). Inhibits the type I interferon activated signaling pathway (PubMed : 30988430). Mechanistically, mono-ADP-ribosylates beta-TrCP/BTRC to promote IFNAR1 ubiquitination and protect BTRC from ubiquitin-proteasome degradation. Additionally, acts as an antiviral factor by cooperating with PARP12 to suppress Zika virus replication, independent of IFNAR1 regulation or intrinsic PARP enzymatic activity (PubMed : 34187568). Instead, facilitates the degradation of viral NS1 and NS3 proteins, potentially disrupting viral replication (PubMed : 34187568).

Sequence similarities

Belongs to the ARTD/PARP family.

Post-translational modifications

Auto-mono-ADP-ribosylated.

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Product protocols

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Target data

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins (PubMed : 25043379, PubMed : 25673562). Plays a role in nuclear envelope stability and nuclear remodeling during spermiogenesis (By similarity). Inhibits the type I interferon activated signaling pathway (PubMed : 30988430). Mechanistically, mono-ADP-ribosylates beta-TrCP/BTRC to promote IFNAR1 ubiquitination and protect BTRC from ubiquitin-proteasome degradation. Additionally, acts as an antiviral factor by cooperating with PARP12 to suppress Zika virus replication, independent of IFNAR1 regulation or intrinsic PARP enzymatic activity (PubMed : 34187568). Instead, facilitates the degradation of viral NS1 and NS3 proteins, potentially disrupting viral replication (PubMed : 34187568).
See full target information PARP11
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