Recombinant human PARP14 protein is a Human Fragment protein, in the 1470 to 1801 aa range, expressed in Baculovirus infected Sf9, with >=83% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >=83% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M H H H H H H G G G S P I L G Y W K I K G L V Q P T R L L L E Y L E E K Y E E H L Y E R D E G D K W R N K K F E L G L E F P N L P Y Y I D G D V K L T Q S M A I I R Y I A D K H N M L G G C P K E R A E I S M L E G A V L D I R Y G V S R I A Y S K D F E T L V D F L S K L P E M L K M F K D R L C H K T Y L N G D H V T H P D F M L Y D A L D V V L Y M D P M C L D A F P K L V C F K K R I E A I P Q I D K Y L K S S K Y I A W P L Q G W Q A T F G G G D H P P K S D P E N L Y F Q G G G E F K E Y Q E L N E L Q K K L N N I S L D H K R P L I K V L G I S R D V M Q A R D E I E A M I K R V R L A K E Q E S R A D C I S E F I E W Q Y N D N N T S H C F N K M T N L K L E D A R R E K K K T V D V K I N H R H Y T V N L N T Y T A T D T K G H S L S V Q R L T K S K V D I P A H W S D K Q Q N F C V V E L L P S D P E Y N T V A S K F N Q T C S H F R I E K I E R I Q N P D L W N S Y Q A K K K T M D A K N G Q T M N E K Q L F H G T D A G S V P H V N R N G F N R S Y A G K N A V A Y G K G T Y F A V N A N Y S A N D T Y S R P D A N G R K H V Y V R V L T G I Y T H G N H S L I V P P S K N P Q N P T D L Y D T V T D N V H H P S L F V A F Y D Y Q A Y P E Y L I T F R K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate residues on target proteins (PubMed:16061477, PubMed:18851833, PubMed:25043379, PubMed:27796300). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed:25043379). Has been shown to catalyze the mono-ADP-ribosylation of STAT1 at 'Glu-657' and 'Glu-705', thus decreasing STAT1 phosphorylation which negatively regulates pro-inflammatory cytokine production in macrophages in response to IFNG stimulation (PubMed:27796300). However, the role of ADP-ribosylation in the prevention of STAT1 phosphorylation has been called into question and it has been suggested that the inhibition of phosphorylation may be the result of sumoylation of STAT1 'Lys-703' (PubMed:29858569). Mono-ADP-ribosylates STAT6; enhancing STAT6-dependent transcription (PubMed:27796300). In macrophages, positively regulates MRC1 expression in response to IL4 stimulation by promoting STAT6 phosphorylation (PubMed:27796300). Mono-ADP-ribosylates PARP9 (PubMed:27796300).
Alternative names
BAL2, KIAA1268, PARP14, Protein mono-ADP-ribosyltransferase PARP14, ADP-ribosyltransferase diphtheria toxin-like 8, B aggressive lymphoma protein 2, Poly [ADP-ribose] polymerase 14, ARTD8, PARP-14
Recommended products
Recombinant human PARP14 protein is a Human Fragment protein, in the 1470 to 1801 aa range, expressed in Baculovirus infected Sf9, with >=83% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=83% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form. Enzyme reaction is conducted using a PARP14 Assay Kit at room temperature for 1 hour.
- Accession
- Q460N5-6
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Preservative: 1.28% Imidazole
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M H H H H H H G G G S P I L G Y W K I K G L V Q P T R L L L E Y L E E K Y E E H L Y E R D E G D K W R N K K F E L G L E F P N L P Y Y I D G D V K L T Q S M A I I R Y I A D K H N M L G G C P K E R A E I S M L E G A V L D I R Y G V S R I A Y S K D F E T L V D F L S K L P E M L K M F K D R L C H K T Y L N G D H V T H P D F M L Y D A L D V V L Y M D P M C L D A F P K L V C F K K R I E A I P Q I D K Y L K S S K Y I A W P L Q G W Q A T F G G G D H P P K S D P E N L Y F Q G G G E F K E Y Q E L N E L Q K K L N N I S L D H K R P L I K V L G I S R D V M Q A R D E I E A M I K R V R L A K E Q E S R A D C I S E F I E W Q Y N D N N T S H C F N K M T N L K L E D A R R E K K K T V D V K I N H R H Y T V N L N T Y T A T D T K G H S L S V Q R L T K S K V D I P A H W S D K Q Q N F C V V E L L P S D P E Y N T V A S K F N Q T C S H F R I E K I E R I Q N P D L W N S Y Q A K K K T M D A K N G Q T M N E K Q L F H G T D A G S V P H V N R N G F N R S Y A G K N A V A Y G K G T Y F A V N A N Y S A N D T Y S R P D A N G R K H V Y V R V L T G I Y T H G N H S L I V P P S K N P Q N P T D L Y D T V T D N V H H P S L F V A F Y D Y Q A Y P E Y L I T F R K
- Accession
- Q460N5
- Protein length
- Fragment
- Predicted molecular weight
- 66.5 kDa
- Amino acids
- 1470 to 1801
- Nature
- Recombinant
- Tags
- GST tag N-Terminus, His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate residues on target proteins (PubMed:16061477, PubMed:18851833, PubMed:25043379, PubMed:27796300). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed:25043379). Has been shown to catalyze the mono-ADP-ribosylation of STAT1 at 'Glu-657' and 'Glu-705', thus decreasing STAT1 phosphorylation which negatively regulates pro-inflammatory cytokine production in macrophages in response to IFNG stimulation (PubMed:27796300). However, the role of ADP-ribosylation in the prevention of STAT1 phosphorylation has been called into question and it has been suggested that the inhibition of phosphorylation may be the result of sumoylation of STAT1 'Lys-703' (PubMed:29858569). Mono-ADP-ribosylates STAT6; enhancing STAT6-dependent transcription (PubMed:27796300). In macrophages, positively regulates MRC1 expression in response to IL4 stimulation by promoting STAT6 phosphorylation (PubMed:27796300). Mono-ADP-ribosylates PARP9 (PubMed:27796300).
- Sequence similarities
Belongs to the ARTD/PARP family.
- Post-translational modifications
Auto-ADP-ribosylated.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PARP14 also known as ADP-ribosyltransferase diphtheria toxin-like 8 (ARTD8) is a member of the poly(ADP-ribose) polymerase (PARP) family. It has a molecular mass approximately 180 kDa and is mainly found in the nucleus though it also localizes to the cytoplasm. This enzyme involves mono-ADP-ribosylation a process attaching ADP-ribose to target proteins which affects protein function and stability. It is expressed in various tissues with significant presence in immune cells.
- Biological function summary
PARP14 participates in cell signaling and immune response regulation. It does not directly form part of a specific protein complex but interacts with several proteins influencing transcription and immune cell differentiation. PARP14 modulates the function of STAT6 a transcription factor vital for IL-4 induced gene expression in Th2 cell differentiation. This modulation plays a significant role in maintaining immune homeostasis and facilitating cellular communication during an immune response.
- Pathways
PARP14 plays an integral role in the JAK-STAT and NF-kB signaling pathways. In the JAK-STAT pathway PARP14 modifies STAT6 activity which affects cytokine signaling and immune responses. Through the NF-kB pathway PARP14 can influence cell survival and inflammation by acting in combination with other PARP family members like PARP1 which shares a role in ADP-ribosylation signaling. These interactions ensure proper regulation of cellular stress responses and transcriptional activities.
- Associated diseases and disorders
PARP14 associations connect to cancer and inflammatory diseases. Its dysregulation can lead to altered immune responses which relate to cancers such as lymphoma. Additionally PARP14 influences inflammatory processes linked to disorders like asthma. Within these diseases abnormal PARP14 activity sometimes involves cross-talk with proteins like STAT6 in cancer pointing to its role in tumor-promoting inflammation and cell proliferation.
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2 product images
Functional Studies - Recombinant human PARP14 protein (ab198628)
Specific activity of ab198628.
SDS-PAGE - Recombinant human PARP14 protein (ab198628)
4-20 % SDS-PAGE analysis of ab198628 with Coomassie staining.
Lane 1: 1 μg ab198628
Lane 2: Protein marker
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