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AB198468

Recombinant human PARP15 protein (GST tag N-Terminus)

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Recombinant human PARP15 protein (GST tag N-Terminus) is a Human Fragment protein, in the 221 to 444 aa range, expressed in Escherichia coli, with >55%, suitable for SDS-PAGE, FuncS.

View Alternative Names

BAL3, PARP15, Protein mono-ADP-ribosyltransferase PARP15, ADP-ribosyltransferase diphtheria toxin-like 7, B-aggressive lymphoma protein 3, Poly [ADP-ribose] polymerase 15, ARTD7, PARP-15

2 Images
Functional Studies - Recombinant human PARP15 protein (GST tag N-Terminus) (AB198468)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PARP15 protein (GST tag N-Terminus) (AB198468)

Specific activity analysis of ab198468.

SDS-PAGE - Recombinant human PARP15 protein (GST tag N-Terminus) (AB198468)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PARP15 protein (GST tag N-Terminus) (AB198468)

10% SDS PAGE of 2 μg ab198468 stained with Coomassie.

Key facts

Purity

>55% SDS-PAGE

Expression system

Escherichia coli

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.

Enzyme reaction is conducted using a PARP15 Assay Kit at room temperature for 30 minutes.

Accession

Q460N3

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab198468 is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
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Sequence info

[{"sequence":"MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSIFYDSMKKRDLSASLNFQSTFSMTTCNLPEHWTDMNHQLFCMVQLEPGQSEYNTIKDKFTRTCSSYAIEKIERIQNAFLWQSYQVKKRQMDIKNDHKNNERLLFHGTDADSVPYVNQHGFNRSCAGKNAVSYGKGTYFAVDASYSAKDTYSKPDSNGRKHMYVVRVLTGVFTKGRAGLVTPPPKNPHNPTDLFDSVTNNTRSPKLFVVFFDNQAYPEYLITFTA","proteinLength":"Fragment","predictedMolecularWeight":"52.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":444,"aminoAcidStart":221,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q460N3","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PARP15 also known as Poly (ADP-Ribose) Polymerase Family Member 15 is an enzyme that acts by adding ADP-ribose polymers to target proteins a process called ADP-ribosylation. This post-translational modification affects protein function and stability. PARP15 has a molecular mass of approximately 75 kDa. It is mainly expressed in the nucleus of cells but can also be observed in the cytoplasm. Its expression varies in different tissues with notable presence in immune-related tissues such as the spleen and lymph nodes.
Biological function summary

PARP15 plays several roles in modulating cellular stress responses and regulating DNA damage repair. It functions as part of a protein complex that involves other PARP family members. PARP15 interacts with chromatin components influencing transcription and maintaining genomic stability. It has shown involvement in modulating pathways that respond to oxidative stress and toxin exposure suggesting a protective role in cellular defense mechanisms.

Pathways

PARP15 is closely linked with the DNA damage response and repair pathways particularly those associated with homologous recombination. In these pathways it functions alongside proteins like BRCA1 and RAD51 ensuring proper DNA repair. PARP15 also has a connection to cell survival signaling pathways such as those regulated by NF-kB thereby contributing to the integration of stress signals into cellular outcomes.

PARP15 has a relation with cancer and autoimmune conditions. Overexpression or abnormal activity of PARP15 correlates with certain cancers where it can affect cancer cell survival through its influence on DNA repair and stress response pathways. Additionally dysfunction in PARP15 or associated pathways may contribute to autoimmune diseases by affecting cell death and inflammatory signaling. Its interaction with other proteins like PARP1 plays a role in these disease states as alterations in these interactions can impact cellular processes and immune regulation.
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Specifications

Form

Liquid

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General info

Function

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins (PubMed : 16061477, PubMed : 25043379, PubMed : 25635049). Acts as a negative regulator of transcription (PubMed : 16061477).

Sequence similarities

Belongs to the ARTD/PARP family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins (PubMed : 16061477, PubMed : 25043379, PubMed : 25635049). Acts as a negative regulator of transcription (PubMed : 16061477).
See full target information PARP15
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