Recombinant human PARP2 protein is a Human Full Length protein, in the 2 to 583 aa range, expressed in Baculovirus infected Sf9, with >=12% purity and suitable for SDS-PAGE, FuncS.
>=12% SDS-PAGE
Baculovirus infected Sf9 cells
GST tag N-Terminus
SDS-PAGE, FuncS
Yes
A A R R R R S T G G G R A R A L N E S K R V N N G N T A P E D S S P A K K T R R C Q R Q E S K K M P V A G G K A N K D R T E D K Q D G M P G R S W A S K R V S E S V K A L L L K G K A P V D P E C T A K V G K A H V Y C E G N D V Y D V M L N Q T N L Q F N N N K Y Y L I Q L L E D D A Q R N F S V W M R W G R V G K M G Q H S L V A C S G N L N K A K E I F Q K K F L D K T K N N W E D R E K F E K V P G K Y D M L Q M D Y A T N T Q D E E E T K K E E S L K S P L K P E S Q L D L R V Q E L I K L I C N V Q A M E E M M M E M K Y N T K K A P L G K L T V A Q I K A G Y Q S L K K I E D C I R A G Q H G R A L M E A C N E F Y T R I P H D F G L R T P P L I R T Q K E L S E K I Q L L E A L G D I E I A I K L V K T E L Q S P E H P L D Q H Y R N L H C A L R P L D H E S Y E F K V I S Q Y L Q S T H A P T H S D Y T M T L L D L F E V E K D G E K E A F R E D L H N R M L L W H G S R M S N W V G I L S H G L R I A P P E A P I T G Y M F G K G I Y F A D M S S K S A N Y C F A S R L K N T G L L L L S E V A L G Q C N E L L E A N P K A E G L L Q G K H S T K G L G K M A P S S A H F V T L N G S T V P L G P A S D T G I L N P D G Y T L N Y N E Y I V Y N P N Q V R M R Y L L K V Q F N F L Q L W
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair (PubMed:10364231, PubMed:25043379, PubMed:27471034, PubMed:30104678, PubMed:32028527, PubMed:32939087, PubMed:34108479, PubMed:34486521, PubMed:34874266). Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units (PubMed:25043379, PubMed:30104678, PubMed:30321391). Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed:32939087). Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1 (PubMed:25043379). Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site (PubMed:28190768, PubMed:32028527, PubMed:34108479, PubMed:34486521, PubMed:34874266). PARP2 initiates the repair of double-strand DNA breaks: recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones, thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks (PubMed:10364231, PubMed:32939087, PubMed:34108479). HPF1 initiates serine ADP-ribosylation but restricts the polymerase activity of PARP2 in order to limit the length of poly-ADP-ribose chains (PubMed:34732825, PubMed:34795260). Specifically mediates formation of branched poly-ADP-ribosylation (PubMed:30104678). Branched poly-ADP-ribose chains are specifically recognized by some factors, such as APLF (PubMed:30104678). In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex (PubMed:27471034, PubMed:29361132).
ADPRT2, ADPRTL2, ADPRTL2, ADPRT2, PARP2, Poly [ADP-ribose] polymerase 2, PARP-2, hPARP-2, ADP-ribosyltransferase diphtheria toxin-like 2, DNA ADP-ribosyltransferase PARP2, NAD(+) ADP-ribosyltransferase 2, Poly[ADP-ribose] synthase 2, Protein poly-ADP-ribosyltransferase PARP2, ARTD2, ADPRT-2, pADPRT-2
Recombinant human PARP2 protein is a Human Full Length protein, in the 2 to 583 aa range, expressed in Baculovirus infected Sf9, with >=12% purity and suitable for SDS-PAGE, FuncS.
>=12% SDS-PAGE
Baculovirus infected Sf9 cells
GST tag N-Terminus
SDS-PAGE, FuncS
Yes
Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Assay Conditions: Enzyme reaction is conducted using a PARP2 Assay Kit at room temperature for 1 hour.
No
Human
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.49% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
A A R R R R S T G G G R A R A L N E S K R V N N G N T A P E D S S P A K K T R R C Q R Q E S K K M P V A G G K A N K D R T E D K Q D G M P G R S W A S K R V S E S V K A L L L K G K A P V D P E C T A K V G K A H V Y C E G N D V Y D V M L N Q T N L Q F N N N K Y Y L I Q L L E D D A Q R N F S V W M R W G R V G K M G Q H S L V A C S G N L N K A K E I F Q K K F L D K T K N N W E D R E K F E K V P G K Y D M L Q M D Y A T N T Q D E E E T K K E E S L K S P L K P E S Q L D L R V Q E L I K L I C N V Q A M E E M M M E M K Y N T K K A P L G K L T V A Q I K A G Y Q S L K K I E D C I R A G Q H G R A L M E A C N E F Y T R I P H D F G L R T P P L I R T Q K E L S E K I Q L L E A L G D I E I A I K L V K T E L Q S P E H P L D Q H Y R N L H C A L R P L D H E S Y E F K V I S Q Y L Q S T H A P T H S D Y T M T L L D L F E V E K D G E K E A F R E D L H N R M L L W H G S R M S N W V G I L S H G L R I A P P E A P I T G Y M F G K G I Y F A D M S S K S A N Y C F A S R L K N T G L L L L S E V A L G Q C N E L L E A N P K A E G L L Q G K H S T K G L G K M A P S S A H F V T L N G S T V P L G P A S D T G I L N P D G Y T L N Y N E Y I V Y N P N Q V R M R Y L L K V Q F N F L Q L W
Full Length
92 kDa
2 to 583
Recombinant
GST tag N-Terminus
Liquid
Affinity purified.
Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair (PubMed:10364231, PubMed:25043379, PubMed:27471034, PubMed:30104678, PubMed:32028527, PubMed:32939087, PubMed:34108479, PubMed:34486521, PubMed:34874266). Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units (PubMed:25043379, PubMed:30104678, PubMed:30321391). Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed:32939087). Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1 (PubMed:25043379). Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site (PubMed:28190768, PubMed:32028527, PubMed:34108479, PubMed:34486521, PubMed:34874266). PARP2 initiates the repair of double-strand DNA breaks: recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones, thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks (PubMed:10364231, PubMed:32939087, PubMed:34108479). HPF1 initiates serine ADP-ribosylation but restricts the polymerase activity of PARP2 in order to limit the length of poly-ADP-ribose chains (PubMed:34732825, PubMed:34795260). Specifically mediates formation of branched poly-ADP-ribosylation (PubMed:30104678). Branched poly-ADP-ribose chains are specifically recognized by some factors, such as APLF (PubMed:30104678). In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex (PubMed:27471034, PubMed:29361132).
Belongs to the ARTD/PARP family.
Auto poly-ADP-ribosylated on serine residues, leading to dissociation of the PARP2-HPF1 complex from chromatin (PubMed:32939087, PubMed:34108479). Poly-ADP-ribosylated by PARP1 (By similarity).
Nucleus
Dry Ice
-80°C
-80°C
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
This supplementary information is collated from multiple sources and compiled automatically.
PARP2 also known as Poly (ADP-ribose) polymerase 2 plays an important role in the repair of damaged DNA. This protein has a molecular mass of approximately 62 kDa. Researchers usually find PARP2 expression in the nucleus primarily in cells undergoing stress or damage responses. The PARP2 protein interacts with substrates during the DNA repair process facilitating the attachment of ADP-ribose polymers to target proteins which is a critical step in the cellular response to DNA strand breaks.
Poly (ADP-ribose) polymerase 2 serves a critical function in maintaining genomic stability. This protein is part of the PARP protein family and operates in conjunction with PARP1. Together they form a complex that senses DNA damage and initiates the repair mechanism. This action helps prevent mutations and genome rearrangements which could otherwise lead to cell death or oncogenesis.
PARP2 contributes significantly to the base excision repair (BER) and homologous recombination (HR) pathways. It collaborates with other repair enzymes and kinases like XRCC1 and DNA-PKcs to effectively mediate the repair processes. The activity of PARP2 ensures prompt and accurate repair of single-strand and double-strand DNA breaks allowing these pathways to maintain cellular homeostasis and integrity.
PARP2 associates prominently with cancer and neurodegenerative diseases. Due to its DNA repair function improper regulation or expression of PARP2 may lead to an increased risk of tumorigenesis making it a target for cancer therapy. Additionally disorders like Alzheimer's disease show an association with PARP2 due to its relationship with oxidative stress and DNA damage. Therapies targeting PARP2 along with PARP1 represent a promising approach in treating these conditions by enhancing DNA repair or inducing synthetic lethality in cancer cells.
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Activity assay using ab198766.
10% SDS-PAGE analysis of 2 μg ab198766 with Coomassie staining.
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