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Recombinant human PARP2 protein is a Human Full Length protein, in the 2 to 583 aa range, expressed in Baculovirus infected Sf9, with >=12% purity and suitable for SDS-PAGE, FuncS.

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Images

Functional Studies - Recombinant human PARP2 protein (AB198766), expandable thumbnail
  • SDS-PAGE - Recombinant human PARP2 protein (AB198766), expandable thumbnail

Publications

Key facts

Purity

>=12% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Amino acid sequence

A A R R R R S T G G G R A R A L N E S K R V N N G N T A P E D S S P A K K T R R C Q R Q E S K K M P V A G G K A N K D R T E D K Q D G M P G R S W A S K R V S E S V K A L L L K G K A P V D P E C T A K V G K A H V Y C E G N D V Y D V M L N Q T N L Q F N N N K Y Y L I Q L L E D D A Q R N F S V W M R W G R V G K M G Q H S L V A C S G N L N K A K E I F Q K K F L D K T K N N W E D R E K F E K V P G K Y D M L Q M D Y A T N T Q D E E E T K K E E S L K S P L K P E S Q L D L R V Q E L I K L I C N V Q A M E E M M M E M K Y N T K K A P L G K L T V A Q I K A G Y Q S L K K I E D C I R A G Q H G R A L M E A C N E F Y T R I P H D F G L R T P P L I R T Q K E L S E K I Q L L E A L G D I E I A I K L V K T E L Q S P E H P L D Q H Y R N L H C A L R P L D H E S Y E F K V I S Q Y L Q S T H A P T H S D Y T M T L L D L F E V E K D G E K E A F R E D L H N R M L L W H G S R M S N W V G I L S H G L R I A P P E A P I T G Y M F G K G I Y F A D M S S K S A N Y C F A S R L K N T G L L L L S E V A L G Q C N E L L E A N P K A E G L L Q G K H S T K G L G K M A P S S A H F V T L N G S T V P L G P A S D T G I L N P D G Y T L N Y N E Y I V Y N P N Q V R M R Y L L K V Q F N F L Q L W

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair (PubMed:10364231, PubMed:25043379, PubMed:27471034, PubMed:30104678, PubMed:32028527, PubMed:32939087, PubMed:34108479, PubMed:34486521, PubMed:34874266). Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units (PubMed:25043379, PubMed:30104678, PubMed:30321391). Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed:32939087). Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1 (PubMed:25043379). Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site (PubMed:28190768, PubMed:32028527, PubMed:34108479, PubMed:34486521, PubMed:34874266). PARP2 initiates the repair of double-strand DNA breaks: recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones, thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks (PubMed:10364231, PubMed:32939087, PubMed:34108479). HPF1 initiates serine ADP-ribosylation but restricts the polymerase activity of PARP2 in order to limit the length of poly-ADP-ribose chains (PubMed:34732825, PubMed:34795260). Specifically mediates formation of branched poly-ADP-ribosylation (PubMed:30104678). Branched poly-ADP-ribose chains are specifically recognized by some factors, such as APLF (PubMed:30104678). In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex (PubMed:27471034, PubMed:29361132).

Alternative names

Recommended products

Recombinant human PARP2 protein is a Human Full Length protein, in the 2 to 583 aa range, expressed in Baculovirus infected Sf9, with >=12% purity and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>=12% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Applications

SDS-PAGE, FuncS

Biological activity

Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.

Assay Conditions: Enzyme reaction is conducted using a PARP2 Assay Kit at room temperature for 1 hour.

Accession
Q9UGN5-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.49% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

Sequence info

Amino acid sequence

A A R R R R S T G G G R A R A L N E S K R V N N G N T A P E D S S P A K K T R R C Q R Q E S K K M P V A G G K A N K D R T E D K Q D G M P G R S W A S K R V S E S V K A L L L K G K A P V D P E C T A K V G K A H V Y C E G N D V Y D V M L N Q T N L Q F N N N K Y Y L I Q L L E D D A Q R N F S V W M R W G R V G K M G Q H S L V A C S G N L N K A K E I F Q K K F L D K T K N N W E D R E K F E K V P G K Y D M L Q M D Y A T N T Q D E E E T K K E E S L K S P L K P E S Q L D L R V Q E L I K L I C N V Q A M E E M M M E M K Y N T K K A P L G K L T V A Q I K A G Y Q S L K K I E D C I R A G Q H G R A L M E A C N E F Y T R I P H D F G L R T P P L I R T Q K E L S E K I Q L L E A L G D I E I A I K L V K T E L Q S P E H P L D Q H Y R N L H C A L R P L D H E S Y E F K V I S Q Y L Q S T H A P T H S D Y T M T L L D L F E V E K D G E K E A F R E D L H N R M L L W H G S R M S N W V G I L S H G L R I A P P E A P I T G Y M F G K G I Y F A D M S S K S A N Y C F A S R L K N T G L L L L S E V A L G Q C N E L L E A N P K A E G L L Q G K H S T K G L G K M A P S S A H F V T L N G S T V P L G P A S D T G I L N P D G Y T L N Y N E Y I V Y N P N Q V R M R Y L L K V Q F N F L Q L W

Accession

Q9UGN5

Protein length

Full Length

Predicted molecular weight

92 kDa

Amino acids

2 to 583

Nature

Recombinant

Tags

GST tag N-Terminus

Specifications

Form

Liquid

Additional notes

Affinity purified.

General info

Function

Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair (PubMed:10364231, PubMed:25043379, PubMed:27471034, PubMed:30104678, PubMed:32028527, PubMed:32939087, PubMed:34108479, PubMed:34486521, PubMed:34874266). Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units (PubMed:25043379, PubMed:30104678, PubMed:30321391). Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed:32939087). Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1 (PubMed:25043379). Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site (PubMed:28190768, PubMed:32028527, PubMed:34108479, PubMed:34486521, PubMed:34874266). PARP2 initiates the repair of double-strand DNA breaks: recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones, thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks (PubMed:10364231, PubMed:32939087, PubMed:34108479). HPF1 initiates serine ADP-ribosylation but restricts the polymerase activity of PARP2 in order to limit the length of poly-ADP-ribose chains (PubMed:34732825, PubMed:34795260). Specifically mediates formation of branched poly-ADP-ribosylation (PubMed:30104678). Branched poly-ADP-ribose chains are specifically recognized by some factors, such as APLF (PubMed:30104678). In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex (PubMed:27471034, PubMed:29361132).

Sequence similarities

Belongs to the ARTD/PARP family.

Post-translational modifications

Auto poly-ADP-ribosylated on serine residues, leading to dissociation of the PARP2-HPF1 complex from chromatin (PubMed:32939087, PubMed:34108479). Poly-ADP-ribosylated by PARP1 (By similarity).

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PARP2 also known as Poly (ADP-ribose) polymerase 2 plays an important role in the repair of damaged DNA. This protein has a molecular mass of approximately 62 kDa. Researchers usually find PARP2 expression in the nucleus primarily in cells undergoing stress or damage responses. The PARP2 protein interacts with substrates during the DNA repair process facilitating the attachment of ADP-ribose polymers to target proteins which is a critical step in the cellular response to DNA strand breaks.

Biological function summary

Poly (ADP-ribose) polymerase 2 serves a critical function in maintaining genomic stability. This protein is part of the PARP protein family and operates in conjunction with PARP1. Together they form a complex that senses DNA damage and initiates the repair mechanism. This action helps prevent mutations and genome rearrangements which could otherwise lead to cell death or oncogenesis.

Pathways

PARP2 contributes significantly to the base excision repair (BER) and homologous recombination (HR) pathways. It collaborates with other repair enzymes and kinases like XRCC1 and DNA-PKcs to effectively mediate the repair processes. The activity of PARP2 ensures prompt and accurate repair of single-strand and double-strand DNA breaks allowing these pathways to maintain cellular homeostasis and integrity.

Associated diseases and disorders

PARP2 associates prominently with cancer and neurodegenerative diseases. Due to its DNA repair function improper regulation or expression of PARP2 may lead to an increased risk of tumorigenesis making it a target for cancer therapy. Additionally disorders like Alzheimer's disease show an association with PARP2 due to its relationship with oxidative stress and DNA damage. Therapies targeting PARP2 along with PARP1 represent a promising approach in treating these conditions by enhancing DNA repair or inducing synthetic lethality in cancer cells.

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2 product images

  • Functional Studies - Recombinant human PARP2 protein (ab198766), expandable thumbnail

    Functional Studies - Recombinant human PARP2 protein (ab198766)

    Activity assay using ab198766.

  • SDS-PAGE - Recombinant human PARP2 protein (ab198766), expandable thumbnail

    SDS-PAGE - Recombinant human PARP2 protein (ab198766)

    10% SDS-PAGE analysis of 2 μg ab198766 with Coomassie staining.

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Product protocols

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