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AB79638

Recombinant human PARP3/IRT1 protein (GST tag N-Terminus)

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Recombinant human PARP3/IRT1 protein (GST tag N-Terminus) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, FuncS.

View Alternative Names

ADPRT3, ADPRTL3, PARP3, Protein mono-ADP-ribosyltransferase PARP3, ADP-ribosyltransferase diphtheria toxin-like 3, DNA ADP-ribosyltransferase PARP3, IRT1, NAD(+) ADP-ribosyltransferase 3, Poly [ADP-ribose] polymerase 3, Poly[ADP-ribose] synthase 3, ARTD3, ADPRT-3, PARP-3, hPARP-3, pADPRT-3

2 Images
Functional Studies - Recombinant human PARP3/IRT1 protein (GST tag N-Terminus) (AB79638)
  • FuncS

Unknown

Functional Studies - Recombinant human PARP3/IRT1 protein (GST tag N-Terminus) (AB79638)

PARP3/IRT1 assay - Specific activity 207.5 U/mg

SDS-PAGE - Recombinant human PARP3/IRT1 protein (GST tag N-Terminus) (AB79638)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PARP3/IRT1 protein (GST tag N-Terminus) (AB79638)

ab79638 on SDS-PAGE, Molecular Weight 87 kDa
10% SDS-PAGE, Coomassie staining
Lane 1 : ab79638 6µg
Lane 2 : Protein marker

Key facts

Purity

>80% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: 207.5 U/mg.

Accession

Q9Y6F1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Complementary Products

Proteins & Peptides

AB198766

Recombinant human PARP2 protein (GST tag N-Terminus)

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Proteins & Peptides

AB79663

Recombinant human PARP1 protein (GST tag N-Terminus)

Functional Studies - Recombinant human PARP1 protein (GST tag N-Terminus) (AB79663)

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Primary Antibodies

AB32064

Anti-Cleaved PARP1 antibody [E51]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [E51] (AB32064)

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Proteins & Peptides

AB271651

Recombinant human PARP7 protein (DDDDK tag N-Terminus)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Protein previously labeled as PARP3.
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9Y6F1","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PARP3/IRT1 also known as Poly(ADP-ribose) polymerase 3 and ADP-ribosyltransferase 1 is a protein involved in DNA repair mechanisms. The protein has a molecular weight of approximately 59 kDa and is expressed in various human tissues predominantly in dividing cells including those in the bone marrow and thymus. PARP3 is one of the members of the PARP family which is known for its role in the base excision repair (BER) of DNA an important process in maintaining genomic stability.
Biological function summary

PARP3 plays a significant role in maintaining chromosomal integrity and facilitating DNA repair. It associates with other proteins as part of the BER complex ensuring efficient repair of DNA single-strand breaks. This protein regulates repair mechanisms by sensing DNA damage and signaling for the recruitment of other DNA repair enzymes. Additionally PARP3 is involved in chromatin remodeling which is critical for accurate DNA repair.

Pathways

PARP3 functions in the DNA damage response (DDR) and the base excision repair pathway. It interacts with other proteins such as XRCC1 an important player in DNA repair to coordinate repair activities. PARP3 modulates the activity of these pathways helping to prevent accumulation of DNA lesions. It plays a part in the signal transduction associated with the cell cycle control which is essential for cellular response to DNA damage and apoptosis.

PARP3 has links to cancer and neurodegenerative diseases. It has been implicated in tumorigenesis due to its role in DNA repair leading to genome stability. Overexpression or mutations might affect the repair process contributing to cancer development. Furthermore PARP3 associates with proteins like p53 a tumor suppressor involved in controlling cell cycle and apoptosis influencing pathways that when altered can lead to neurodegenerative conditions. Understanding PARP3 functions could aid in the development of therapeutic strategies targeting these pathways.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins and plays a key role in the response to DNA damage (PubMed : 16924674, PubMed : 19354255, PubMed : 20064938, PubMed : 21211721, PubMed : 21270334, PubMed : 23742272, PubMed : 24598253, PubMed : 25043379, PubMed : 28447610). Mediates mono-ADP-ribosylation of glutamate, aspartate or lysine residues on target proteins (PubMed : 20064938, PubMed : 25043379). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed : 25043379). Involved in DNA repair by mediating mono-ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism, such as histone H2B, XRCC5 and XRCC6 (PubMed : 16924674, PubMed : 24598253). ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed : 16924674, PubMed : 21211721, PubMed : 21270334). Involved in single-strand break repair by catalyzing mono-ADP-ribosylation of histone H2B on 'Glu-2' (H2BE2ADPr) of nucleosomes containing nicked DNA (PubMed : 27530147). Cooperates with the XRCC5-XRCC6 (Ku80-Ku70) heterodimer to limit end-resection thereby promoting accurate NHEJ (PubMed : 24598253). Suppresses G-quadruplex (G4) structures in response to DNA damage (PubMed : 28447610). Associates with a number of DNA repair factors and is involved in the response to exogenous and endogenous DNA strand breaks (PubMed : 16924674, PubMed : 21211721, PubMed : 21270334). Together with APLF, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ) (PubMed : 21211721). May link the DNA damage surveillance network to the mitotic fidelity checkpoint (PubMed : 16924674). Acts as a negative regulator of immunoglobulin class switch recombination, probably by controlling the level of AICDA /AID on the chromatin (By similarity). In addition to proteins, also able to ADP-ribosylate DNA : mediates DNA mono-ADP-ribosylation of DNA strand break termini via covalent addition of a single ADP-ribose moiety to a 5'- or 3'-terminal phosphate residues in DNA containing multiple strand breaks (PubMed : 29361132, PubMed : 29520010).

Sequence similarities

Belongs to the ARTD/PARP family.

Post-translational modifications

Auto-mono-ADP-ribosylated.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins and plays a key role in the response to DNA damage (PubMed : 16924674, PubMed : 19354255, PubMed : 20064938, PubMed : 21211721, PubMed : 21270334, PubMed : 23742272, PubMed : 24598253, PubMed : 25043379, PubMed : 28447610). Mediates mono-ADP-ribosylation of glutamate, aspartate or lysine residues on target proteins (PubMed : 20064938, PubMed : 25043379). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed : 25043379). Involved in DNA repair by mediating mono-ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism, such as histone H2B, XRCC5 and XRCC6 (PubMed : 16924674, PubMed : 24598253). ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed : 16924674, PubMed : 21211721, PubMed : 21270334). Involved in single-strand break repair by catalyzing mono-ADP-ribosylation of histone H2B on 'Glu-2' (H2BE2ADPr) of nucleosomes containing nicked DNA (PubMed : 27530147). Cooperates with the XRCC5-XRCC6 (Ku80-Ku70) heterodimer to limit end-resection thereby promoting accurate NHEJ (PubMed : 24598253). Suppresses G-quadruplex (G4) structures in response to DNA damage (PubMed : 28447610). Associates with a number of DNA repair factors and is involved in the response to exogenous and endogenous DNA strand breaks (PubMed : 16924674, PubMed : 21211721, PubMed : 21270334). Together with APLF, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ) (PubMed : 21211721). May link the DNA damage surveillance network to the mitotic fidelity checkpoint (PubMed : 16924674). Acts as a negative regulator of immunoglobulin class switch recombination, probably by controlling the level of AICDA /AID on the chromatin (By similarity). In addition to proteins, also able to ADP-ribosylate DNA : mediates DNA mono-ADP-ribosylation of DNA strand break termini via covalent addition of a single ADP-ribose moiety to a 5'- or 3'-terminal phosphate residues in DNA containing multiple strand breaks (PubMed : 29361132, PubMed : 29520010).
See full target information PARP3
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