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AB271651

Recombinant human PARP7 protein (Tagged)

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Recombinant human PARP7 protein (Tagged) is a Human Fragment protein, in the 400 to 657 aa range, expressed in Baculovirus infected Sf9 cells, with >33%, suitable for SDS-PAGE, FuncS.

View Alternative Names

PARP7, TIPARP, Protein mono-ADP-ribosyltransferase TIPARP, ADP-ribosyltransferase diphtheria toxin-like 14, Poly [ADP-ribose] polymerase 7, TCDD-inducible poly [ADP-ribose] polymerase, ARTD14, PARP-7

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Functional Studies - Recombinant human PARP7 protein (Tagged) (AB271651)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PARP7 protein (Tagged) (AB271651)

Specific activity of ab271651 was >40 pmol/min/μg.

SDS-PAGE - Recombinant human PARP7 protein (Tagged) (AB271651)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PARP7 protein (Tagged) (AB271651)

SDS-PAGE analysis of ab271651.

Key facts

Purity

>33% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

DDDDK tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific Activity: >40 pmol/min/μg

Assay Conditions: Assay was done according to PARP7 assay kit protocol. First, histone proteins are coated on a 96-well plate. Next, the biotinylated substrate is incubated with an assay buffer that contains the PARP7 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Accession

Q7Z3E1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"KRRPLFRSCFILLPYLQTLGGVPTQAPPPLEATSSSQIICPDGVTSANFYPETWVYMHPSQDFIQVPVSAEDKSYRIIYNLFHKTVPEFKYRILQILRVQNQFLWEKYKRKKEYMNRKMFGRDRIINERHLFHGTSQDVVDGICKHNFDPRVCGKHATMFGQGSYFAKKASYSHNFSKKSSKGVHFMFLAKVLTGRYTMGSHGMRRPPPVNPGSVTSDLYDSCVDNFFEPQIFVIFNDDQSYPYFVIQYEEVSNTVSI","proteinLength":"Fragment","predictedMolecularWeight":"31 kDa","actualMolecularWeight":null,"aminoAcidEnd":657,"aminoAcidStart":400,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q7Z3E1","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PARP7 also known as Poly (ADP-ribose) polymerase 7 is an enzyme that serves a significant role in cellular processes. PARP7 belongs to the PARP family with a molecular mass of around 77 kDa. Found in the nucleus PARP7 performs its function largely within cells of the immune system. Structural analysis reveals that PARP7 has a DNA-binding domain as well as a catalytic domain necessary for its enzymatic activity which involves adding ADP-ribose units to proteins.
Biological function summary

PARP7 influences gene expression and the cellular response to stress. It participates in regulating transcription by modifying other proteins through ADP-ribosylation impacting gene expression directly. PARP7 is not a part of a larger protein complex but collaborates with components in the immune system pathway to facilitate the innate immune response. Its function helps to maintain the equilibrium between different cellular processes and stress handling ensuring the proper cellular function.

Pathways

PARP7 integrates into the interferon and oxidative stress response pathways. The PARP7 enzyme interacts with proteins like interferon regulatory factors and plays a role in modulating antiviral responses ensuring efficient communication within cells when they encounter stress or infection. Additionally through its activity PARP7 influences pathways involving members of the same PARP protein family highlighting its interconnected role in cellular signaling processes.

PARP7 has links to certain cancers and autoimmune diseases. Aberrant PARP7 activity has been observed in some cancer types with research indicating that abnormal expression or mutations may contribute to tumor progression. In autoimmune diseases PARP7 might affect the immune response potentially altering outcomes in conditions such as lupus. In these contexts PARP7 might interact with specific proteins like STAT1 or other regulatory proteins which have key roles in both disease development and progression.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate, aspartate and cysteine residues on target proteins (PubMed : 23275542, PubMed : 25043379, PubMed : 30373764). Acts as a negative regulator of AHR by mediating mono-ADP-ribosylation of AHR, leading to inhibit transcription activator activity of AHR (PubMed : 23275542, PubMed : 30373764).

Sequence similarities

Belongs to the ARTD/PARP family.

Post-translational modifications

Auto-mono-ADP-ribosylated.

Subcellular localisation

Nucleus

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Product protocols

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Target data

ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate, aspartate and cysteine residues on target proteins (PubMed : 23275542, PubMed : 25043379, PubMed : 30373764). Acts as a negative regulator of AHR by mediating mono-ADP-ribosylation of AHR, leading to inhibit transcription activator activity of AHR (PubMed : 23275542, PubMed : 30373764).
See full target information TIPARP
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