Recombinant Human Parvalbumin Protein Standard (His tag) is a Human Fragment protein, expressed in Escherichia coli, with >=80% purity and suitable for SDS-PAGE, sELISA.
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Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, sELISA
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.
Alternative names
Parvalbumin alpha, PVALB
Recommended products
Recombinant Human Parvalbumin Protein Standard (His tag) is a Human Fragment protein, expressed in Escherichia coli, with >=80% purity and suitable for SDS-PAGE, sELISA.
Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, sELISA
- Biologically active
- No
- Accession
- P20472-1
- Animal free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- Accession
- P20472
- Protein length
- Fragment
- Predicted molecular weight
- 13.9 kDa
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.
- Sequence similarities
Belongs to the parvalbumin family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Parvalbumin sometimes referred to as PV is a calcium-binding protein approximately 12 kDa in size. It is recognized for its roles in muscle and neuronal tissues. Within the nervous system parvalbumin is prominently expressed in fast-spiking GABAergic interneurons while in muscle tissues it is present in fast-twitch muscle fibers. Other popular descriptors include anti PV and parvalbumins. These proteins play important roles in the regulation of cellular calcium levels.
- Biological function summary
Parvalbumin helps modulate calcium ion concentration which is key in muscle relaxation and neurotransmission. It acts by buffering calcium ions and is not part of a larger protein complex. By impacting calcium transients parvalbumin affects processes like synaptic plasticity and the rapid contractions of muscle fibers. This is especially significant in the brain where rapid neuronal firing is needed and in muscles where quick recovery is essential.
- Pathways
Parvalbumin is intimately linked with calcium signaling pathways as well as the regulation of muscle contraction. It ensures proper calcium ion homeostasis thereby aiding muscle relaxation after contraction. In the nervous system calbindin proteins parallel some of parvalbumin's functions participating in calcium ion buffering and modulation of neuron excitability. The balanced action of these proteins helps maintain cellular functions in both neurons and muscles.
- Associated diseases and disorders
Insufficient parvalbumin expression may link to epilepsy and schizophrenia. Deficits in this protein could interfere with normal neurotransmission and muscle relaxation cycles. Parvalbumin deficiency influences neurotransmitter release affecting synaptic transmission which may exacerbate symptoms in these conditions. In disease mechanisms proteins such as calbindin may indirectly relate with parvalbumin showing similar patterns of dysregulation and compensating for its absence in calcium handling.
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2 product images
SDS-PAGE - Recombinant Human Parvalbumin Protein Standard (His tag) (ab316551)
SDS-PAGE analysis of ab316551 under reducing conditions for 2ug protein
Sandwich ELISA - Recombinant Human Parvalbumin Protein Standard (His tag) (ab316551)
Sandwich ELISA with the capture antibody dilution at 2 μg/mL and detector antibody dilution at 0.5 μg/mL.
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Product protocols
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