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AB161560

Recombinant Human PASK protein (GST tag N-Terminus)

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Recombinant Human PASK protein (GST tag N-Terminus) is a Human Fragment protein, in the 1 to 100 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

KIAA0135, PASK, PAS domain-containing serine/threonine-protein kinase, PAS-kinase, PASKIN, hPASK

1 Images
SDS-PAGE - Recombinant Human PASK protein (GST tag N-Terminus) (AB161560)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PASK protein (GST tag N-Terminus) (AB161560)

ab161560 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q96RG2

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MEDGGLTAFEEDQRCLSQSLPLPVSAEGPAAQTTAEPSRSFSSAHRHLSRRNGLSRLCQSRTALSEDRWSSYCLSSLAAQNICTSKLHCPAAPEHTDPSE","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":100,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q96RG2","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PASK also known as PAS domain-containing serine/threonine-protein kinase is a protein with a mass of approximately 142 kDa. This kinase expresses in various tissues including the liver heart and skeletal muscle. PASK contains PAS (Per-ARNT-Sim) domains that allow adaptation to environmental and cellular signals. The protein kinase plays an important role in regulating energy balance and metabolism through phosphorylation of downstream targets.
Biological function summary

PASK functions as a metabolic sensor that links changes in cellular energy levels to the regulation of gene expression and metabolic processes. It does not form part of a well-characterized complex but interacts with other proteins to exert its effects. PASK influences insulin secretion in pancreatic beta-cells and regulates glucose homeostasis. It plays a role in lipid metabolism by modulating the activity of various metabolic enzymes and transcription factors.

Pathways

PASK participates in the insulin signaling pathway and nutrient-sensitive pathways influencing energy homeostasis and cellular metabolism. It interacts closely with proteins such as AMP-activated protein kinase (AMPK) and mTOR which are themselves central regulators of cellular energy balance. These interactions place PASK as a node in the complex network controlling energy storage and expenditure.

PASK has been linked to metabolic diseases such as type 2 diabetes and obesity. Altered expression or mutations in PASK can disrupt metabolic regulation leading to insulin resistance. Additionally studies have shown connections between PASK and cardiovascular diseases due to its involvement in lipid metabolism and glucose homeostasis. It interacts with proteins like peroxisome proliferator-activated receptor gamma (PPARγ) which plays a role in fat storage and glucose metabolism highlighting PASK's importance in these conditions.
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Specifications

Form

Liquid

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General info

Function

Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis : regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate in respiratory regulation.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Post-translational modifications

Autophosphorylated on Thr-1161 and Thr-1165. Autophosphorylation is activated by phospholipids.

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Product protocols

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Target data

Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis : regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate in respiratory regulation.
See full target information PASK
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