Recombinant Human PAX7 protein is a Human Fragment protein, in the 411 to 521 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
G G L D S A T S I S A S C S Q R A D S I K P G D S L P T S Q A Y C P P T Y S T T G Y S V D P V A G Y Q Y G Q Y G Q S E C L V P W A S P V P I P S P T P R A S C L F M E S Y K V V S G W G M S I S Q M E K L K S S Q M E Q F T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes (Recombinant protein). |
Target data
Function
Transcription factor that is involved in the regulation of muscle stem cells proliferation, playing a role in myogenesis and muscle regeneration.
Alternative names
HUP1, PAX7, Paired box protein Pax-7, HuP1
Recommended products
Recombinant Human PAX7 protein is a Human Fragment protein, in the 411 to 521 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P23759-3
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- G G L D S A T S I S A S C S Q R A D S I K P G D S L P T S Q A Y C P P T Y S T T G Y S V D P V A G Y Q Y G Q Y G Q S E C L V P W A S P V P I P S P T P R A S C L F M E S Y K V V S G W G M S I S Q M E K L K S S Q M E Q F T
- Accession
- P23759
- Protein length
- Fragment
- Predicted molecular weight
- 38.21 kDa
- Amino acids
- 411 to 521
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Transcription factor that is involved in the regulation of muscle stem cells proliferation, playing a role in myogenesis and muscle regeneration.
- Sequence similarities
Belongs to the paired homeobox family.
- Post-translational modifications
Acetylation at Lys-105 and Lys-139 by KAT8 is required for high-level transcription factor activity. Deacetylated by SIRT2.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PAX7 or paired box 7 is a member of the paired box (PAX) family of transcription factors. Alternative names for PAX7 include PAX7 protein and PAX7 FACS. This protein has a molecular weight of approximately 57 kDa. PAX7 is mainly expressed in muscle precursor cells where it plays an important role in regulating muscle development. Researchers commonly detect it through PAX7 staining techniques in laboratory settings.
- Biological function summary
PAX7 functions are important for muscle cell proliferation and differentiation. It works within a transcriptional complex where it partners with other proteins to promote satellite cell maintenance. Satellite cells are essential for muscle regeneration and PAX7 regulates their self-renewal and differentiation. The protein also modulates various genetic targets that affect the muscle cellular environment ensuring proper muscle function and repair.
- Pathways
PAX7 is involved in the myogenic regulatory pathways that guide muscle development and regeneration. It interacts with important proteins like MyoD and Myf5 to influence these processes. These pathways facilitate the conversion of satellite cells into mature muscle fibers important during muscle growth and healing. PAX7’s function in these pathways ensures satellite cells remain active and capable of muscle repair throughout an organism's life.
- Associated diseases and disorders
PAX7’s dysfunction can lead to muscle-related conditions such as rhabdomyosarcoma and certain muscle dystrophies. In rhabdomyosarcoma improper regulation of PAX7 leads to unchecked cellular proliferation contributing to tumor growth. This disorder links PAX7 to other proteins in the tumorigenic pathway such as PAX3 which similarly influences muscle cell behavior in pathological states. Identifying PAX7 alterations can guide diagnosis and therapeutic strategies in muscle-associated disorders.
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1 product image
SDS-PAGE - Recombinant Human PAX7 protein (ab114237)
12.5% SDS-PAGE showing ab114237 at approximately 38.21kDa stained with Coomassie Blue.
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Product protocols
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