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AB123196

Recombinant Human PBK/SPK protein

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(1 Publication)

Recombinant Human PBK/SPK protein is a Human Full Length protein, in the 1 to 322 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

TOPK, PBK, Lymphokine-activated killer T-cell-originated protein kinase, Cancer/testis antigen 84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, T-LAK cell-originated protein kinase, CT84, SPK

1 Images
SDS-PAGE - Recombinant Human PBK/SPK protein (AB123196)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PBK/SPK protein (AB123196)

15% SDS-PAGE showing ab123196 (3μg).
(Molecular size on SDS-PAGE will appear higher).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q96KB5

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as PBK
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMEGISNFKTPSKLSEKKKSVLCSTPTINIPASPFMQKLGFGTGVNVYLMKRSPRGLSHSPWAVKKINPICNDHYRSVYQKRLMDEAKILKSLHHPNIVGYRAFTEANDGSLCLAMEYGGEKSLNDLIEERYKASQDPFPAAIILKVALNMARGLKYLHQEKKLLHGDIKSSNVVIKGDFETIKICDVGVSLPLDENMTVTDPEACYIGTEPWKPKEAVEENGVITDKADIFAFGLTLWEMMTLSIPHINLSNDDDDEDKTFDESDFDDEAYYAALGTRPPINMEELDESYQKVIELFSVCTNEDPKDRPSAAHIVEALETDV","proteinLength":"Full Length","predictedMolecularWeight":"38.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":322,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96KB5","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PBK/SPK also known as PDZ-binding kinase or Cdc2-related kinase is a serine/threonine protein kinase with an approximate molecular weight of 47 kDa. This protein is involved in cell division playing an important role in mitotic processes. PBK/SPK is expressed in various tissues including significant levels in testis and placenta often in proliferating cell types. It also exhibits higher expression in cancerous tissues suggesting a role in tumorigenesis.
Biological function summary

PBK/SPK facilitates cell cycle progression and cellular proliferation. This protein is not typically part of larger complexes but it partners with other molecules during cell division to ensure proper mitotic spindle formation and chromosome segregation. Its expression levels increase during the G2/M phase of the cell cycle indicating its involvement in cell cycle regulation. In cancer it often shows overexpression contributing to unregulated proliferation of cancer cells.

Pathways

PBK/SPK is associated with critical cell division pathways such as the MAPK signaling pathway. This involvement impacts cellular responses to growth signals and stress. PBK can phosphorylate and interact with proteins like p38 MAPK which further modulates signal transduction related to cellular growth and survival. Through these pathways PBK/SPK helps manage the balance between cell division and apoptosis particularly under stress conditions.

PBK/SPK has strong correlations with cancer especially glioblastoma and cervical cancer. Overexpression of PBK often links to poor prognosis in these conditions. In cancerous settings PBK interacts with proteins such as p53 and c-Myc influencing tumor progression and resistance to therapy. PBK can modulate oncogenic pathways providing a potential target for therapeutic intervention in tumor proliferation and survival.
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Specifications

Form

Liquid

Additional notes

ab123196 is purified using conventional chromatography techniques.

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General info

Function

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

Post-translational modifications

Phosphorylated; in a cell-cycle dependent manner at mitosis.

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Product protocols

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Target data

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
See full target information PBK
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 38:1136-1150 PubMed30237440

2018

A reciprocal feedback between the PDZ binding kinase and androgen receptor drives prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Y Warren,Charlie E Massie,Kate Watt,Katarina Luko,Folake Orafidiya,Luke A Selth,Hisham Mohammed,Brinder S Chohan,Suraj Menon,Ajoeb Baridi,Wanfeng Zhao,Carles Escriu,Thanakorn Pungsrinont,Clive D'Santos,Xiaoping Yang,Chris Taylor,Arham Qureshi,Vincent R Zecchini,Greg L Shaw,Scott M Dehm,Ian G Mills,Jason S Carroll,Wayne D Tilley,Iain J McEwan,Aria Baniahmad,David E Neal,Mohammad Asim
View all publications
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