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AB101217

Recombinant Human PBLD protein

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Recombinant Human PBLD protein is a Human Full Length protein, in the 1 to 288 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

MAWBP, PBLD, Phenazine biosynthesis-like domain-containing protein, MAWD-binding protein, Unknown protein 32 from 2D-page of liver tissue, MAWDBP

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SDS-PAGE - Recombinant Human PBLD protein (AB101217)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PBLD protein (AB101217)

ab101217 at 3 μg analysed by 15% SDS PAGE.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P30039

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMKLPIFIADAFTARAFRGNPAAVCLLENELDEDMHQKIAREMNLSETAFIRKLHPTDNFAQSSCFGLRWFTPASEVPLCGHATLASAAVLFHKIKNMNSTLTFVTLSGELRARRAEDGIVLDLPLYPAHPQDFHEVEDLIKTAIGNTLVQDICYSPDTQKLLVRLSDVYNRSFLENLKVNTENLLQVENTGKVKGLILTLKGEPGGQTQAFDFYSRYFAPWVGVAEDPVTGSAHAVLSSYWSQHLGKKEMHAFQCSHRGGELGISLRPDGRVDIRGGAAVVLEGTLTA","proteinLength":"Full Length","predictedMolecularWeight":"33.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":288,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P30039","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PBLD also known as Phenazine Biosynthesis-Like Protein Domain is a protein with an approximate mass of 40 kDa. It is expressed in various tissues including the liver and kidney. PBLD possesses structural domains that resemble those found in the phenazine biosynthesis family suggesting its involvement in redox processes. Researchers have yet to fully clarify its mechanical role but its conserved domains hint at significant enzymatic functions in cellular metabolism.
Biological function summary

The role of PBLD appears linked to modulation of oxidative stress responses and metabolic processes. PBLD is part of larger protein complexes that regulate intracellular metabolic pathways. Its involvement in these complexes enables it to impact cellular balance in response to environmental or cellular stresses. This function suggests a protective role in maintaining cellular homeostasis and potentially implicates PBLD in detoxification processes within the cell.

Pathways

PBLD participates in metabolic and oxidative stress response pathways specifically the glutathione metabolism and the pentose phosphate pathway. It interacts with other proteins involved in these processes such as NADPH-producing enzymes and components of the oxidative defense system. These interactions allow PBLD to contribute to cellular redox balance and metabolic flux connecting it to essential cellular functions.

PBLD has shown associations with liver disorders and certain metabolic syndromes. For example altered expression of PBLD may relate to hepatic steatosis in conjunction with proteins such as CYP450 enzymes affecting liver metabolism. It also exhibits potential connections to metabolic syndrome where it might influence pathways involving insulin resistance and lipid metabolism indicating its broader role in metabolic health conditions.
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Specifications

Form

Liquid

Additional notes

ab101217 was purified using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

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General info

Sequence similarities

Belongs to the PhzF family.

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Product protocols

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Target data

See full target information PBLD
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Secondary Antibodies

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Primary Antibodies

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