Recombinant Human PC4 protein is a Human Full Length protein, in the 1 to 127 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M P K S K E L V S S S S S G S D S D S E V D K K L K R K K Q V A P E K P V K K Q K T G E T S R A L S S S K Q S S S S R D D N M F Q I G K M R Y V S V R D F K G K V L I D I R E Y W M D P E G E M K P G R K G I S L N P E Q W S Q L K E Q I S D I D D A V R K L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
Alternative names
PC4, RPO2TC1, SUB1, Activated RNA polymerase II transcriptional coactivator p15, Positive cofactor 4, SUB1 homolog, p14
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Recombinant Human PC4 protein is a Human Full Length protein, in the 1 to 127 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P53999-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M P K S K E L V S S S S S G S D S D S E V D K K L K R K K Q V A P E K P V K K Q K T G E T S R A L S S S K Q S S S S R D D N M F Q I G K M R Y V S V R D F K G K V L I D I R E Y W M D P E G E M K P G R K G I S L N P E Q W S Q L K E Q I S D I D D A V R K L
- Accession
- P53999
- Protein length
- Full Length
- Predicted molecular weight
- 16.5 kDa
- Amino acids
- 1 to 127
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab95890 is purified using conventional chromatography techniques.
General info
- Function
General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
- Sequence similarities
Belongs to the transcriptional coactivator PC4 family.
- Post-translational modifications
Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PC4 also known as Sub1 is a transcriptional coactivator protein. Its molecular weight is approximately 20 kDa. The protein is expressed in various tissues with particularly high expression in the liver and heart. PC4 facilitates transcription by interacting with basal transcription factors. It bonds with single-stranded DNA enhancing RNA polymerase II's ability to start transcription.
- Biological function summary
Many experts note PC4's significant role in transcriptional regulation and chromatin structure dynamics. It operates as part of a multifunctional complex that regulates gene expression. PC4 stabilizes transcriptional pre-initiation complexes which helps initiate gene transcription. Its capacity to modulate chromatin can also impact DNA repair and replication processes emphasizing its diverse roles beyond transcriptional activation.
- Pathways
Transcription regulation processes often feature PC4 prominently. It interacts within the transcriptional machinery alongside proteins such as TFIIB and TFIIH. Additionally PC4 participates in chromatin remodeling pathways which affect DNA accessibility and gene transcription. This protein's interaction with other transcription factors establishes it as a versatile component in gene expression pathways.
- Associated diseases and disorders
Researchers link PC4 to cancer and cardiac hypertrophy. In cancer PC4 can influence oncogene activation and tumor suppressor pathways interacting with proteins like p53 in these contexts. Studies also suggest PC4 involvement in cardiac hypertrophy by affecting genes associated with heart muscle enlargement where it functions alongside other cardiac stress-responsive proteins. These associations highlight PC4's potential as a therapeutic target in various pathologies.
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SDS-PAGE - Recombinant Human PC4 protein (ab95890)
15% SDS-PAGE analysis of 3μg ab95890.
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