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AB182818

Recombinant Human PCDH12 protein

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Recombinant Human PCDH12 protein is a Human Fragment protein, in the 25 to 718 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

UNQ395/PRO731, PCDH12, Protocadherin-12, Vascular cadherin-2, Vascular endothelial cadherin-2, VE-cad-2, VE-cadherin-2

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9NPG4

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MASMTGGQQMGRGHHHHHHGNLYFQGGEFQEVTTLTVKYQVSEEVPSGTVIGKLSQELGREERRRQAGAAFQVLQLPQALPIQVDSEEGLLSTGRRLDREQLCRQWDPCLVSFDVLATGDLALIHVEIQVLDINDHQPRFPKGEQELEISESASLRTRIPLDRALDPDTGPNTLHTYTLSPSEHFALDVIVGPDETKHAELIVVKELDREIHSFFDLVLTAYDNGNPPKSGTSLVKVNVLDSNDNSPAFAESSLALEIQEDAAPGTLLIKLTATDPDQGPNGEVEFFLSKHMPPEVLDTFSIDAKTGQVILRRPLDYEKNPAYEVDVQARDLGPNPIPAHCKVLIKVLDVNDNIPSIHVTWASQPSLVSEALPKDSFIALVMADDLDSGNNGLVHCWLSQELGHFRLKRTNGNTYMLLTNATLDREQWPKYTLTLLAQDQGLQPLSAKKQLSIQISDINDNAPVFEKSRYEVSTRENNLPSLHLITIKAHDADLGINGKVSYRIQDSPVAHLVAIDSNTGEVTAQRSLNYEEMAGFEFQVIAEDSGQPMLASSVSVWVSLLDANDNAPEVVQPVLSDGKASLSVLVNASTGHLLVPIETPNGLGPAGTDTPPLATHSSRPFLLTTIVARDADSGANGEPLYSIRSGNEAHLFILNPHTGQLFVNVTNASSLIGSEWELEIVVEDQGSPPLQTRALLRVMFVTSVDHLRDSARKPGALSMSMLT","proteinLength":"Fragment","predictedMolecularWeight":"79.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":718,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NPG4","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCDH12 also known as Protocadherin 12 belongs to the protocadherin family which is part of the cadherin superfamily. PCDH12 has a molecular mass of about 120 kDa. This protein is expressed in endothelial cells and certain types of smooth muscle tissues. Researchers have identified it in the central nervous system and specific fetal tissues suggesting a role in early development and vascular formation.
Biological function summary

PCDH12 contributes to cell-cell adhesion mechanisms. It plays a significant part in maintaining cellular architecture and establishing cell connectivity. Although not definitively confirmed as part of a larger complex its function aligns with other cadherins that mediate homophilic binding to stabilize cell interactions. It also participates in the development of the blood-brain barrier by supporting endothelial junctions.

Pathways

PCDH12 functions within the signaling networks related to cell adhesion and migration. It influences angiogenesis through its role in adherens junction pathways. This involvement links it to proteins such as VE-cadherin which are critical for vascular integrity. Additionally PCDH12 interacts with pathways that manage neural development potentially cooperating with other neural-specific cadherins.

PCDH12 shows connections to cerebrovascular diseases and certain cancer types. In stroke research altered PCDH12 expression correlates with blood-brain barrier dysfunction. Studies also indicate a possible involvement in tumor progression where its regulation affects cellular proliferation pathways. PCDH12 interacts with proteins like beta-catenin which may facilitate changes in cell signaling during tumorigenesis.
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Specifications

Form

Liquid

Additional notes

ab182818 was expressed in E.coli as inclusion bodies, refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified.

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General info

Function

Cellular adhesion molecule that may play an important role in cell-cell interactions at interendothelial junctions (By similarity). Acts as a regulator of cell migration, probably via increasing cell-cell adhesion (PubMed : 21402705). Promotes homotypic calcium-dependent aggregation and adhesion and clusters at intercellular junctions (By similarity). Unable to bind to catenins, weakly associates with the cytoskeleton (By similarity).

Post-translational modifications

Protocadherin-12. Cleaved by ADAM10 close to the transmembrane domain to release the Protocadherin-12, secreted form in the serum. Cleavage results in reduced cellular adhesion in a cell migration assay.

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Product protocols

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Target data

Cellular adhesion molecule that may play an important role in cell-cell interactions at interendothelial junctions (By similarity). Acts as a regulator of cell migration, probably via increasing cell-cell adhesion (PubMed : 21402705). Promotes homotypic calcium-dependent aggregation and adhesion and clusters at intercellular junctions (By similarity). Unable to bind to catenins, weakly associates with the cytoskeleton (By similarity).
See full target information PCDH12
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