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AB198485

Recombinant Human PCGF6 protein (GST tag N-Terminus)

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Recombinant Human PCGF6 protein (GST tag N-Terminus) is a Human Full Length protein, in the 2 to 350 aa range, expressed in Baculovirus infected Sf9 cells, with >86%, suitable for SDS-PAGE, FuncS.

View Alternative Names

MBLR, RNF134, PCGF6, Polycomb group RING finger protein 6, Mel18 and Bmi1-like RING finger, RING finger protein 134

1 Images
SDS-PAGE - Recombinant Human PCGF6 protein (GST tag N-Terminus) (AB198485)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PCGF6 protein (GST tag N-Terminus) (AB198485)

4-20% SDS PAGE analysis of ab198485 (2.1 μg).

Key facts

Purity

>86% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

No

Accession

Q9BYE7

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.48% Tris, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Complementary Products

Primary Antibodies

AB200038

Anti-PCGF6 antibody [EPR19450]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PCGF6 antibody [EPR19450] (AB200038)

  • 0
  • 0
Proteins & Peptides

AB313361

Recombinant Human WFDC2 Protein (His tag)

Mass Spectrometry - Recombinant Human WFDC2 Protein (His tag) (AB313361)

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Proteins & Peptides

AB95933

Recombinant human XIAP protein (DDDDK tag N-Terminus)

Functional Studies - Recombinant human XIAP protein (DDDDK tag N-Terminus) (AB95933)

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Proteins & Peptides

AB217407

Recombinant human Vitronectin/S-Protein (Animal Free)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab198485 is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

This product was previously labelled as RING6a

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Sequence info

[{"sequence":"MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFKDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDPLVPRGSPGEFEGVAVVTAGSVGAAKTEGAAALPPPPPVSPPALTPAPAAGEEGPAPLSETGAPGCSGSRPPELEPERSLGRFRGRFEDEDEELEEEEELEEEEEEEEEDMSHFSLRLEGGRQDSEDEEERLINLSELTPYILCSICKGYLIDATTITECLHTFCKSCIVRHFYYSNRCPKCNIVVHQTQPLYNIRLDRQLQDIVYKLVINLEEREKKQMHDFYKERGLEVPKPAVPQPVPSSKGRSKKVLESVFRIPPELDMSLLLEFIGANEGTGHFKPLEKKFVRVSGEATIGHVEKFLRRKMGLDPACQVDIICGDHLLEQYQTLREIRRAIGDAAMQDGLLVLHYGLVVSPLKIT","proteinLength":"Full Length","predictedMolecularWeight":"65.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":350,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9BYE7","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCGF6 also known as Polycomb group RING finger protein 6 plays a role in regulating gene expression. This protein with a molecular mass of about 36 kDa is part of the Polycomb Group (PcG) proteins that epigenetically silence genes preventing their expression. Scientists have observed PCGF6 expression in embryonic stem cells as well as in various tissues hinting at its broad potential impact on cellular processes. The protein contains a RING finger motif which suggests it functions as an E3 ubiquitin ligase tagging other proteins for modification or degradation.
Biological function summary

PCGF6 participates in transcriptional repression through its involvement in the non-canonical Polycomb Repressive Complex 1 (PRC1). The protein acts by aiding the assembly of PRC1 alongside other constituents such as RING1B to modify histones and maintain stem cell characteristics. This activity is important for cell differentiation and maintaining the balance between pluripotency and lineage specification during development. Disruptions in PCGF6 function may influence epigenetic landscapes significantly thereby impacting cellular identity and behavior.

Pathways

PCGF6 integrates its activity with key biological systems such as the Wnt signaling and the polycomb repression pathways. Within these pathways PCGF6 interacts with core PRC1 components like RING1B to affect gene silencing and chromatin remodeling. These interactions allow the protein to serve as a critical node in controlling genetic hierarchies that regulate embryogenesis and other developmental processes. The pathway network involving PCGF6 and PRC1 provides essential insights into the fine-tuning of gene expressions needed for cellular differentiation.

Aberrations in PCGF6 expression or function have connections to cancer and developmental disorders. Altered PCGF6 activity can lead to dysregulated expression of genes that are normally repressed fostering oncogenic or detrimental developmental outcomes. In the context of cancer its interaction with components such as BMI1 helps to drive tumor progression by supporting unchecked cell proliferation. Furthermore understanding these interactions highlights potential therapeutic avenues by targeting the components influenced by PCGF6 offering opportunities for better management of these diseases.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Transcriptional repressor (PubMed : 12167161). May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase (PubMed : 17320162). Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed : 12167161). Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (PubMed : 26151332).

Post-translational modifications

Phosphorylated during mitosis. Phosphorylated on Ser-30 by CDK7 in vitro.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Transcriptional repressor (PubMed : 12167161). May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase (PubMed : 17320162). Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed : 12167161). Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (PubMed : 26151332).
See full target information PCGF6
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Product promise

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