Recombinant Human PCGF6 protein is a Human Full Length protein, in the 2 to 350 aa range, expressed in Baculovirus infected Sf9, with >=86% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >=86% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- No
Amino acid sequence
M S P I L G Y W K I K G L V Q P T R L L L E Y L E E K Y E E H L Y E R D E G D K W R N K K F E L G L E F P N L P Y Y I D G D V K L T Q S M A I I R Y I A D K H N M L G G C P K E R A E I S M L E G A V L D I R Y G V S R I A Y S K D F E T L K V D F L S K L P E M L K M F K D R L C H K T Y L N G D H V T H P D F M L Y D A L D V V L Y M D P M C L D A F P K L V C F K K R I E A I P Q I D K Y L K S S K Y I A W P L Q G W Q A T F G G G D H P P K S D P L V P R G S P G E F E G V A V V T A G S V G A A K T E G A A A L P P P P P V S P P A L T P A P A A G E E G P A P L S E T G A P G C S G S R P P E L E P E R S L G R F R G R F E D E D E E L E E E E E L E E E E E E E E E D M S H F S L R L E G G R Q D S E D E E E R L I N L S E L T P Y I L C S I C K G Y L I D A T T I T E C L H T F C K S C I V R H F Y Y S N R C P K C N I V V H Q T Q P L Y N I R L D R Q L Q D I V Y K L V I N L E E R E K K Q M H D F Y K E R G L E V P K P A V P Q P V P S S K G R S K K V L E S V F R I P P E L D M S L L L E F I G A N E G T G H F K P L E K K F V R V S G E A T I G H V E K F L R R K M G L D P A C Q V D I I C G D H L L E Q Y Q T L R E I R R A I G D A A M Q D G L L V L H Y G L V V S P L K I T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Transcriptional repressor (PubMed:12167161). May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase (PubMed:17320162). Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:12167161). Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (PubMed:26151332).
Alternative names
MBLR, RNF134, PCGF6, Polycomb group RING finger protein 6, Mel18 and Bmi1-like RING finger, RING finger protein 134
Recommended products
Recombinant Human PCGF6 protein is a Human Full Length protein, in the 2 to 350 aa range, expressed in Baculovirus infected Sf9, with >=86% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=86% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- No
- Accession
- Q9BYE7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.48% Tris, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M S P I L G Y W K I K G L V Q P T R L L L E Y L E E K Y E E H L Y E R D E G D K W R N K K F E L G L E F P N L P Y Y I D G D V K L T Q S M A I I R Y I A D K H N M L G G C P K E R A E I S M L E G A V L D I R Y G V S R I A Y S K D F E T L K V D F L S K L P E M L K M F K D R L C H K T Y L N G D H V T H P D F M L Y D A L D V V L Y M D P M C L D A F P K L V C F K K R I E A I P Q I D K Y L K S S K Y I A W P L Q G W Q A T F G G G D H P P K S D P L V P R G S P G E F E G V A V V T A G S V G A A K T E G A A A L P P P P P V S P P A L T P A P A A G E E G P A P L S E T G A P G C S G S R P P E L E P E R S L G R F R G R F E D E D E E L E E E E E L E E E E E E E E E D M S H F S L R L E G G R Q D S E D E E E R L I N L S E L T P Y I L C S I C K G Y L I D A T T I T E C L H T F C K S C I V R H F Y Y S N R C P K C N I V V H Q T Q P L Y N I R L D R Q L Q D I V Y K L V I N L E E R E K K Q M H D F Y K E R G L E V P K P A V P Q P V P S S K G R S K K V L E S V F R I P P E L D M S L L L E F I G A N E G T G H F K P L E K K F V R V S G E A T I G H V E K F L R R K M G L D P A C Q V D I I C G D H L L E Q Y Q T L R E I R R A I G D A A M Q D G L L V L H Y G L V V S P L K I T
- Accession
- Q9BYE7
- Protein length
- Full Length
- Predicted molecular weight
- 65.7 kDa
- Amino acids
- 2 to 350
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Transcriptional repressor (PubMed:12167161). May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase (PubMed:17320162). Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:12167161). Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (PubMed:26151332).
- Post-translational modifications
Phosphorylated during mitosis. Phosphorylated on Ser-30 by CDK7 in vitro.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Notes
ab198485 is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
This product was previously labelled as RING6a
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PCGF6 also known as Polycomb group RING finger protein 6 plays a role in regulating gene expression. This protein with a molecular mass of about 36 kDa is part of the Polycomb Group (PcG) proteins that epigenetically silence genes preventing their expression. Scientists have observed PCGF6 expression in embryonic stem cells as well as in various tissues hinting at its broad potential impact on cellular processes. The protein contains a RING finger motif which suggests it functions as an E3 ubiquitin ligase tagging other proteins for modification or degradation.
- Biological function summary
PCGF6 participates in transcriptional repression through its involvement in the non-canonical Polycomb Repressive Complex 1 (PRC1). The protein acts by aiding the assembly of PRC1 alongside other constituents such as RING1B to modify histones and maintain stem cell characteristics. This activity is important for cell differentiation and maintaining the balance between pluripotency and lineage specification during development. Disruptions in PCGF6 function may influence epigenetic landscapes significantly thereby impacting cellular identity and behavior.
- Pathways
PCGF6 integrates its activity with key biological systems such as the Wnt signaling and the polycomb repression pathways. Within these pathways PCGF6 interacts with core PRC1 components like RING1B to affect gene silencing and chromatin remodeling. These interactions allow the protein to serve as a critical node in controlling genetic hierarchies that regulate embryogenesis and other developmental processes. The pathway network involving PCGF6 and PRC1 provides essential insights into the fine-tuning of gene expressions needed for cellular differentiation.
- Associated diseases and disorders
Aberrations in PCGF6 expression or function have connections to cancer and developmental disorders. Altered PCGF6 activity can lead to dysregulated expression of genes that are normally repressed fostering oncogenic or detrimental developmental outcomes. In the context of cancer its interaction with components such as BMI1 helps to drive tumor progression by supporting unchecked cell proliferation. Furthermore understanding these interactions highlights potential therapeutic avenues by targeting the components influenced by PCGF6 offering opportunities for better management of these diseases.
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1 product image
SDS-PAGE - Recombinant Human PCGF6 protein (ab198485)
4-20% SDS PAGE analysis of ab198485 (2.1 μg).
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