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AB114699

Recombinant Human PCM1 protein (GST tag N-Terminus)

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Recombinant Human PCM1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 530 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

Pericentriolar material 1 protein, PCM-1, hPCM-1, PCM1

1 Images
SDS-PAGE - Recombinant Human PCM1 protein (GST tag N-Terminus) (AB114699)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PCM1 protein (GST tag N-Terminus) (AB114699)

12.5% SDS-PAGE showing ab114699 at approximately 84.41 kDa stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, WB, ELISA

applications

Biologically active

No

Accession

Q15154

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB72443

Anti-PCM1 antibody

Immunoprecipitation - Anti-PCM1 antibody (AB72443)

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Proteins & Peptides

AB159505

Recombinant Human SHBG protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human SHBG protein (GST tag N-Terminus) (AB159505)

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  • 0
Proteins & Peptides

AB276441

Recombinant human IL-28B protein (His tag)

Functional Studies - Recombinant human IL-28B protein (His tag) (AB276441)

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  • 0
Proteins & Peptides

AB165007

Recombinant Human CMTM1 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human CMTM1 protein (GST tag N-Terminus) (AB165007)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(Recombinant protein).</p>" } } }
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Sequence info

[{"sequence":"MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQ","proteinLength":"Full Length","predictedMolecularWeight":"84.41 kDa","actualMolecularWeight":null,"aminoAcidEnd":530,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q15154","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCM1 (Pericentriolar Material 1) also known as the PCM-1 protein functions mechanically in the organization of centrosomes and microtubules. This mechanistic role is linked with its name due to its positioning around the centrosome a critical cellular structure for division. The PCM1 protein shows an approximate mass of 228 kDa. It is expressed broadly in various tissues but mainly in organs with high cellular turnover including the testis thymus and heart. Notably PCM1 serves as a scaffold protein anchoring various centrosomal proteins important for their correct assembly and positioning.
Biological function summary

The PCM1 protein plays an important role in the microtubule stabilization and cell cycle progression. This protein becomes part of the larger pericentriolar material complex which supports the necessary structural platform for centrosome and cilia function. PCM1 assists in docking other proteins like centrin centrin-binding proteins and pericentriolar satellites in the complex ensuring the intricacies of ciliary trafficking and centrosome cohesion are well-maintained. It helps in regulating processes critical to normal cell polarity and division.

Pathways

PCM1 protein integrates pathway activities relating to not only microtubule dynamism but also cellular motility. It is involved in the centrosome-cilia interface important for signal transduction and cellular homeostasis. PCM1 interacts with key pathway proteins such as CEP290 and OFD1 which are significant for ciliogenesis thereby influencing cellular responses to extracellular cues. These pathways directly tie the mechanics of PCM1 to essential biological responses including cell signaling pathways that ensure normal embryonic development and cell differentiation.

PCM1 plays a relevant role in the context of ciliopathies and certain cancers. Abnormalities or mutations in PCM1 have associations with disorders such as Bardet-Biedl Syndrome a ciliopathy characterized by malfunctioning cilia. Aberrant PCM1 expressions or mutations also have links to leukemia through its implication in the ETV6-PCM1 fusion gene. The interplay between PCM1 and proteins such as ETV6 in leukemia highlights its impact on chromosomal translocations and the potential muscular pathway misregulation leading to malignancy.
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Specifications

Form

Liquid

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General info

Function

Required for centrosome assembly and function (PubMed : 12403812, PubMed : 15659651, PubMed : 16943179). Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2 (PubMed : 12403812, PubMed : 15659651). Required to anchor microtubules to the centrosome (PubMed : 12403812, PubMed : 15659651). Also involved in cilium biogenesis by recruiting the BBSome, a ciliary protein complex involved in cilium biogenesis, to the centriolar satellites (PubMed : 20551181, PubMed : 24121310, PubMed : 27979967). Recruits the tubulin polyglutamylase complex (TPGC) to centriolar satellites (PubMed : 34782749).

Sequence similarities

Belongs to the PCM1 family.

Post-translational modifications

Ubiquitinated. Undergoes monoubiquitination catalyzed by the E3 ubiquitin-protein ligase MIB1 in proliferating cells, preventing cilia formation (PubMed:24121310). Monoubiquitination by MIB1 is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, resulting in cilia formation initiation (PubMed:24121310).. Variant Ser-159 is phosphorylated.. Phosphorylated on multiple serine and threonine residues by DYRK3 during the G2-to-M transition, after the nuclear-envelope breakdown (PubMed:29973724). Phosphorylation by DYRK3 promotes disassembly of pericentriolar material (PubMed:29973724). Phosphorylation at Ser-372 mediated by PLK4 is required to maintain the integrity of centriolar satellites (PubMed:30804208).

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Product protocols

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Target data

Required for centrosome assembly and function (PubMed : 12403812, PubMed : 15659651, PubMed : 16943179). Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2 (PubMed : 12403812, PubMed : 15659651). Required to anchor microtubules to the centrosome (PubMed : 12403812, PubMed : 15659651). Also involved in cilium biogenesis by recruiting the BBSome, a ciliary protein complex involved in cilium biogenesis, to the centriolar satellites (PubMed : 20551181, PubMed : 24121310, PubMed : 27979967). Recruits the tubulin polyglutamylase complex (TPGC) to centriolar satellites (PubMed : 34782749).
See full target information PCM1
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