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AB95914

Recombinant Human PCMT1 protein (His tag N-Terminus)

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Recombinant Human PCMT1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 227 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Protein-L-isoaspartate(D-aspartate) O-methyltransferase, PIMT, L-isoaspartyl protein carboxyl methyltransferase, Protein L-isoaspartyl/D-aspartyl methyltransferase, Protein-beta-aspartate methyltransferase, PCMT1

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SDS-PAGE - Recombinant Human PCMT1 protein (His tag N-Terminus) (AB95914)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PCMT1 protein (His tag N-Terminus) (AB95914)

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P22061

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl

storage-buffer

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Complementary Products

Primary Antibodies

AB97446

Anti-PCMT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCMT1 antibody (AB97446)

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Biochemicals

AB141153

Galanin (1-29) (rat, mouse), Galanin receptor agonist

Functional Studies - Galanin (1-29) (rat, mouse), Galanin receptor agonist (AB141153)

  • 0
  • 0
Proteins & Peptides

AB71666

Recombinant human PKR protein

Functional Studies - Recombinant human PKR protein (AB71666)

  • 0
  • 0
Primary Antibodies

AB79405

Anti-PDCD4 antibody [EPR3432]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 antibody [EPR3432] (AB79405)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMAWKSGGASHSELIHNLRKNGIIKTDKVFEVMLATDRSHYAKCNPYMDSPQSIGFQATISAPHMHAYALELLFDQLHEGAKALDVGSGSGILTACFARMVGCTGKVIGIDHIKELVDDSINNVRKDDPTLLSSGRVQLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGGNQMLEQYDKLQDGSIKMKPLMGVIYVPLTDKEKQWSRWK","proteinLength":"Full Length","predictedMolecularWeight":"28.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":227,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P22061","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PCMT1 protein also known as protein-L-isoaspartate (D-aspartate) O-methyltransferase catalyzes the repair of damaged proteins by recognizing and converting abnormal L-isoaspartyl residues to normal L-aspartyl residues. This enzymatic activity is important for maintaining protein integrity and function. The purified form of PCMT1 has a mass of approximately 24 kDa. It is ubiquitously expressed in various tissues with high levels found in the liver and brain emphasizing its essential role across different body systems.
Biological function summary

The PCMT1 enzyme plays a significant role in cellular repair mechanisms by rejuvenating proteins that have been altered due to spontaneous deamidation or isomerization. This process is critical for preserving the longevity of proteins and preventing cellular aging and dysfunction. PCMT1 does not generally form part of a complex but works by interacting directly with protein substrates that contain abnormal isoaspartyl residues. It functions independently in its activity and this specificity highlights its importance in maintaining cellular homeostasis.

Pathways

PCMT1 is an integral component of protein repair pathways specifically the pathways that manage protein maintenance and degradation. These pathways help prevent the accumulation of dysfunctional proteins thereby preserving cell function and survival. PCMT1 activity relates closely to proteins such as CHIP and ubiquitin-proteasome pathway elements which also manage protein quality control within the cell. Together these proteins ensure accurate protein folding and turnover.

PCMT1 activity connects to neurodegenerative disorders like Alzheimer's disease and demyelinating conditions such as multiple sclerosis. The deficiency or dysfunction in PCMT1 could lead to abnormal accumulation of damaged proteins causing cellular stress and contributing to disease pathology. In the context of these conditions PCMT1 has also been linked to other proteins like beta-amyloid in Alzheimer's illustrating the cascading effect that modified protein repair can have on disease development and progression.
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Specifications

Form

Liquid

Additional notes

ab95914 is purified using conventional chromatography techniques.

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General info

Function

Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins (PubMed : 3167043, PubMed : 6469980). Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin C-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein (By similarity).

Sequence similarities

Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.

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Product protocols

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Target data

Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins (PubMed : 3167043, PubMed : 6469980). Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin C-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein (By similarity).
See full target information PCMT1
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Product promise

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