Recombinant Human PCNA protein (His tag) is a Human Full Length protein, in the 1 to 261 aa range, expressed in Escherichia coli, with >=95% purity, <= 0.1 EU/mg endotoxin level and suitable for SDS-PAGE, HPLC, MS.
Images
Key facts
- Purity
>=95% HPLC
- Endotoxin level
<= 0.1 EU/mg
- Expression system
Escherichia coli
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, HPLC, MS
- Biologically active
No
Amino acid sequence
M F E A R L V Q G S I L K K V L E A L K D L I N E A C W D I S S S G V N L Q S M D S S H V S L V Q L T L R S E G F D T Y R C D R N L A M G V N L T S M S K I L K C A G N E D I I T L R A E D N A D T L A L V F E A P N Q E K V S D Y E M K L M D L D V E Q L G I P E Q E Y S C V V K M P S G E F A R I C R D L S H I G D A V V I S C A K D G V K F S A S G E L G N G N I K L S Q T S N V D K E E E A V T I E M N E P V Q L T F A L R Y L N F F T K A T P L S S T V T L S M S A D V P L V V E Y K I A D M G H L K Y Y L A P K I E D E E G S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).
Alternative names
Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
Recommended products
Recombinant Human PCNA protein (His tag) is a Human Full Length protein, in the 1 to 261 aa range, expressed in Escherichia coli, with >=95% purity, <= 0.1 EU/mg endotoxin level and suitable for SDS-PAGE, HPLC, MS.
Alternative names
Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
Key facts
- Purity
>=95% HPLC
- Endotoxin level
<= 0.1 EU/mg
- Expression system
Escherichia coli
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, HPLC, MS
- Biologically active
No
- Accession
- P12004-1
- Animal free
Yes
- Species
Human
- Reconstitution
Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
M F E A R L V Q G S I L K K V L E A L K D L I N E A C W D I S S S G V N L Q S M D S S H V S L V Q L T L R S E G F D T Y R C D R N L A M G V N L T S M S K I L K C A G N E D I I T L R A E D N A D T L A L V F E A P N Q E K V S D Y E M K L M D L D V E Q L G I P E Q E Y S C V V K M P S G E F A R I C R D L S H I G D A V V I S C A K D G V K F S A S G E L G N G N I K L S Q T S N V D K E E E A V T I E M N E P V Q L T F A L R Y L N F F T K A T P L S S T V T L S M S A D V P L V V E Y K I A D M G H L K Y Y L A P K I E D E E G S
- Accession
- Protein length
Full Length
- Predicted molecular weight
29.83 kDa
- Actual molecular weight
29.83 kDa
- Amino acids
1 to 261
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Lyophilized
- Additional notes
SDS-PAGE >=95%
General info
- Function
Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).
- Sequence similarities
Belongs to the PCNA family.
- Post-translational modifications
Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
Ambient
- Appropriate long-term storage conditions
Ambient
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.
- Biological function summary
This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.
- Pathways
PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.
- Associated diseases and disorders
PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.
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3 product images
Mass Spectrometry - Recombinant Human PCNA protein (His tag) (ab307191)
Mass determination by ESI-TOF.
Predicted MW is 29832.96 Da (+/- 10 Da by ESI-TOF). Observed MW is 29832.64 Da.
HPLC - Recombinant Human PCNA protein (His tag) (ab307191)
HPLC analysis of ab307191
SDS-PAGE - Recombinant Human PCNA protein (His tag) (ab307191)
SDS-page analysis of ab307191
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