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AB202191

Recombinant Human PCOLCE protein (denatured) (His tag N-Terminus)

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Recombinant Human PCOLCE protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 26 to 449 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

PCPE1, PCOLCE, Procollagen C-endopeptidase enhancer 1, Procollagen COOH-terminal proteinase enhancer 1, Type 1 procollagen C-proteinase enhancer protein, Type I procollagen COOH-terminal proteinase enhancer, PCPE-1, Procollagen C-proteinase enhancer 1

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SDS-PAGE - Recombinant Human PCOLCE protein (denatured) (AB202191)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PCOLCE protein (denatured) (AB202191)

15% SDS-PAGE analysis of ab202191 (3 μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q15113

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSQTPNYTRPVFLCGGDVKGESGYVASEGFPNLYPPNKECIWTITVPEGQTVSLSFRVFDLELHPACRYDALEVFAGSGTSGQRLGRFCGTFRPAPLVAPGNQVTLRMTTDEGTGGRGFLLWYSGRATSGTEHQFCGGRLEKAQGTLTTPNWPESDYPPGISCSWHIIAPPDQVIALTFEKFDLEPDTYCRYDSVSVFNGAVSDDSRRLGKFCGDAVPGSISSEGNELLVQFVSDLSVTADGFSASYKTLPRGTAKEGQGPGPKRGTEPKVKLPPKSQPPEKTEESPSAPDAPTCPKQCRRTGTLQSNFCASSLVVTATVKSMVREPGEGLAVTVSLIGAYKTGGLDLPSPPTGASLKFYVPCKQCPPMKKGVSYLLMGQVEENRGPVLPPESFVVLHRPNQDQILTNLSKRKCPSQPVRAAASQD","proteinLength":"Full Length","predictedMolecularWeight":"47.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":449,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q15113","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCOLCE known as Procollagen C-endopeptidase enhancer is a glycoprotein with an approximate mass of 55 kDa. It resides mainly in the extracellular matrix playing a role in collagen processing. PCOLCE enhances the activity of the enzyme procollagen C-proteinase which is critical for converting procollagen to collagen by cleaving its C-terminal propeptide. PCOLCE expression occurs in tissues such as skin tendons and cartilage where collagen is abundant.
Biological function summary

PCOLCE serves as an enhancer of enzyme activity important for proper collagen maturation. This protein strongly associates with the biosynthesis of collagen fibrils being part of the larger complex responsible for proper collagen assembly. The effective regulation of collagen fibrils is essential for maintaining structural integrity and strength of various tissues such as the skin and bone matrix.

Pathways

PCOLCE plays an important role in the collagen biosynthesis and modifying pathways. It collaborates closely with other proteins like BMP1 (Bone Morphogenetic Protein 1) which also participates in the procollagen processing activity. Through its precise regulation PCOLCE affects tissue repair and development pathways ensuring efficient collagen synthesis and deposition critical for functional extracellular matrix formation.

Mutations or dysregulation in PCOLCE can potentially relate to connective tissue disorders such as Ehlers-Danlos syndrome. In these conditions improper collagen processing occurs affecting tissue elasticity and strength. Studies suggest that abnormal PCOLCE activity may also influence fibrosis where excessive collagen deposition leads to tissue and organ dysfunction. In these contexts PCOLCE interaction with proteins like BMP1 and other collagen-modifying enzymes is significant affecting the progression of such disorders.
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Specifications

Form

Liquid

Additional notes

ab202191 was purified using conventional chromatography techniques.

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General info

Function

Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity.. C-terminal processed part of PCPE (CT-PCPE) may have an metalloproteinase inhibitory activity.

Post-translational modifications

C-terminally processed at multiple positions.

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Product protocols

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Target data

Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity.. C-terminal processed part of PCPE (CT-PCPE) may have an metalloproteinase inhibitory activity.
See full target information PCOLCE
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Primary Antibodies

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