Recombinant Human PCQAP / MED15 protein is a Human Full Length protein, in the 1 to 674 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M D V S G Q E T D W R S T A F R Q K L V S Q I E D A M R K A G V A H S K S S K D M E S H V F L K A K T R D E Y L S L V A R L I I H F R D I H N K K S Q A S V S A Q L Q L Q Q V A L Q Q Q Q Q Q Q Q F Q Q Q Q Q A A L Q Q Q Q Q Q Q Q Q F Q A Q Q S A M Q Q Q F Q A V V Q Q Q Q Q L Q Q Q Q Q Q Q H L I K L H H Q N Q Q Q I Q Q Q Q Q Q L Q R I A Q L Q L Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q A L Q A Q P P I Q Q P P M Q Q P Q P P P S Q A L P Q Q L Q Q M H H T Q H H Q P P P Q P Q Q P P V A Q N Q P S Q L P P Q S Q T Q P L V S Q A Q A L P G Q M L Y T Q P P L K F V R A P M V V Q Q P P V Q P Q V Q Q Q Q T A V Q T A Q A A Q M V A P G V Q V S Q S S L P M L S S P S P G Q Q V Q T P Q S M P P P P Q P S P Q P G Q P S S Q P N S N V S S G P A P S P S S F L P S P S P Q P S Q S P V T A R T P Q N F S V P S P G P L N T P V N P S S V M S P A G S S Q A E E Q Q Y L D K L K Q L S K Y I E P L R R M I N K I D K N E D R K K D L S K M K S L L D I L T D P S K R C P L K T L Q K C E I A L E K L K N D M A V P T P P P P P V P P T K Q Q Y L C Q P L L D A V L A N I R S P V F N H S L Y R T F V P A M T A I H G P P I T A P V V C T R K R R L E D D E R Q S I P S V L Q G E V A R L D P K F L V N L D P S H C S N N G T V H L I C K L D D K D L P S V P P L E L S V P A D Y P A Q S P L W I D R Q W Q Y D A N P F L Q S V H R C M T S R L L Q L P D K H S V T A L L N T W A Q S V H Q A C L S A A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway.
Alternative names
ARC105, CTG7A, PCQAP, TIG1, TNRC7, MED15, Mediator of RNA polymerase II transcription subunit 15, Activator-recruited cofactor 105 kDa component, CTG repeat protein 7a, Mediator complex subunit 15, Positive cofactor 2 glutamine/Q-rich-associated protein, TPA-inducible gene 1 protein, Trinucleotide repeat-containing gene 7 protein, PC2 glutamine/Q-rich-associated protein, TIG-1
Recommended products
Recombinant Human PCQAP / MED15 protein is a Human Full Length protein, in the 1 to 674 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q96RN5-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M D V S G Q E T D W R S T A F R Q K L V S Q I E D A M R K A G V A H S K S S K D M E S H V F L K A K T R D E Y L S L V A R L I I H F R D I H N K K S Q A S V S A Q L Q L Q Q V A L Q Q Q Q Q Q Q Q F Q Q Q Q Q A A L Q Q Q Q Q Q Q Q Q F Q A Q Q S A M Q Q Q F Q A V V Q Q Q Q Q L Q Q Q Q Q Q Q H L I K L H H Q N Q Q Q I Q Q Q Q Q Q L Q R I A Q L Q L Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q A L Q A Q P P I Q Q P P M Q Q P Q P P P S Q A L P Q Q L Q Q M H H T Q H H Q P P P Q P Q Q P P V A Q N Q P S Q L P P Q S Q T Q P L V S Q A Q A L P G Q M L Y T Q P P L K F V R A P M V V Q Q P P V Q P Q V Q Q Q Q T A V Q T A Q A A Q M V A P G V Q V S Q S S L P M L S S P S P G Q Q V Q T P Q S M P P P P Q P S P Q P G Q P S S Q P N S N V S S G P A P S P S S F L P S P S P Q P S Q S P V T A R T P Q N F S V P S P G P L N T P V N P S S V M S P A G S S Q A E E Q Q Y L D K L K Q L S K Y I E P L R R M I N K I D K N E D R K K D L S K M K S L L D I L T D P S K R C P L K T L Q K C E I A L E K L K N D M A V P T P P P P P V P P T K Q Q Y L C Q P L L D A V L A N I R S P V F N H S L Y R T F V P A M T A I H G P P I T A P V V C T R K R R L E D D E R Q S I P S V L Q G E V A R L D P K F L V N L D P S H C S N N G T V H L I C K L D D K D L P S V P P L E L S V P A D Y P A Q S P L W I D R Q W Q Y D A N P F L Q S V H R C M T S R L L Q L P D K H S V T A L L N T W A Q S V H Q A C L S A A
- Accession
- Q96RN5
- Protein length
- Full Length
- Amino acids
- 1 to 674
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway.
- Sequence similarities
Belongs to the Mediator complex subunit 15 family.
- Post-translational modifications
Ubiquitinated by TRIM11, leading to proteasomal degradation.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PCQAP also known as MED15 is a subunit of the Mediator complex an essential coactivator for transcription. MED15 helps facilitate the transcription process in eukaryotic cells by bridging transcription factors and RNA polymerase II. Its molecular mass is approximately 100 kDa. The protein is expressed widely in various tissues suggesting it has multiple roles in cellular processes.
- Biological function summary
PCQAP/MED15 is important in regulating gene expression. It forms part of the large Mediator complex which interacts with various transcription factors to initiate and control chromosomal transcription. This interaction aids in the precise regulation of gene expression necessary for cell differentiation and development. Its function is quite important in the transcription machinery within the cell nucleus.
- Pathways
PCQAP/MED15 integrates into the signal transduction and gene expression pathways. It has an involvement in the Wnt signaling pathway a system essential for embryonic development and cell growth. MED15 acts in conjunction with other proteins such as β-catenin which transduces the Wnt signal to the nucleus where MED15 participates in the transcriptional regulation of target genes.
- Associated diseases and disorders
PCQAP/MED15 has connections to cancer and certain neurological disorders. Its misregulation or mutation can lead to transcriptional dysregulation contributing to the onset of cancers by affecting genes that control cell proliferation. In neurological disorders PCQAP/MED15 impacts the expression of genes involved in neural functions with possible ties to disorders like Huntington's disease. The protein interacts with p53 a well-known tumor suppressor protein highlighting its role in disease development and progression.
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1 product image
SDS-PAGE - Recombinant Human PCQAP / MED15 protein (ab162600)
ab162600 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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