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AB162600

Recombinant Human PCQAP / MED15 protein (GST tag N-Terminus)

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Recombinant Human PCQAP / MED15 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 674 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

ARC105, CTG7A, PCQAP, TIG1, TNRC7, MED15, Mediator of RNA polymerase II transcription subunit 15, Activator-recruited cofactor 105 kDa component, CTG repeat protein 7a, Mediator complex subunit 15, Positive cofactor 2 glutamine/Q-rich-associated protein, TPA-inducible gene 1 protein, Trinucleotide repeat-containing gene 7 protein, PC2 glutamine/Q-rich-associated protein, TIG-1

1 Images
SDS-PAGE - Recombinant Human PCQAP / MED15 protein (GST tag N-Terminus) (AB162600)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PCQAP / MED15 protein (GST tag N-Terminus) (AB162600)

ab162600 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q96RN5

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Primary Antibodies

AB181158

Anti-PCQAP / MED15 antibody [EPR13309]

RabMAb|Recombinant|Trial Size

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MDVSGQETDWRSTAFRQKLVSQIEDAMRKAGVAHSKSSKDMESHVFLKAKTRDEYLSLVARLIIHFRDIHNKKSQASVSAQLQLQQVALQQQQQQQQFQQQQQAALQQQQQQQQQFQAQQSAMQQQFQAVVQQQQQLQQQQQQQHLIKLHHQNQQQIQQQQQQLQRIAQLQLQQQQQQQQQQQQQQQQALQAQPPIQQPPMQQPQPPPSQALPQQLQQMHHTQHHQPPPQPQQPPVAQNQPSQLPPQSQTQPLVSQAQALPGQMLYTQPPLKFVRAPMVVQQPPVQPQVQQQQTAVQTAQAAQMVAPGVQVSQSSLPMLSSPSPGQQVQTPQSMPPPPQPSPQPGQPSSQPNSNVSSGPAPSPSSFLPSPSPQPSQSPVTARTPQNFSVPSPGPLNTPVNPSSVMSPAGSSQAEEQQYLDKLKQLSKYIEPLRRMINKIDKNEDRKKDLSKMKSLLDILTDPSKRCPLKTLQKCEIALEKLKNDMAVPTPPPPPVPPTKQQYLCQPLLDAVLANIRSPVFNHSLYRTFVPAMTAIHGPPITAPVVCTRKRRLEDDERQSIPSVLQGEVARLDPKFLVNLDPSHCSNNGTVHLICKLDDKDLPSVPPLELSVPADYPAQSPLWIDRQWQYDANPFLQSVHRCMTSRLLQLPDKHSVTALLNTWAQSVHQACLSAA","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":674,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q96RN5","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCQAP also known as MED15 is a subunit of the Mediator complex an essential coactivator for transcription. MED15 helps facilitate the transcription process in eukaryotic cells by bridging transcription factors and RNA polymerase II. Its molecular mass is approximately 100 kDa. The protein is expressed widely in various tissues suggesting it has multiple roles in cellular processes.
Biological function summary

PCQAP/MED15 is important in regulating gene expression. It forms part of the large Mediator complex which interacts with various transcription factors to initiate and control chromosomal transcription. This interaction aids in the precise regulation of gene expression necessary for cell differentiation and development. Its function is quite important in the transcription machinery within the cell nucleus.

Pathways

PCQAP/MED15 integrates into the signal transduction and gene expression pathways. It has an involvement in the Wnt signaling pathway a system essential for embryonic development and cell growth. MED15 acts in conjunction with other proteins such as β-catenin which transduces the Wnt signal to the nucleus where MED15 participates in the transcriptional regulation of target genes.

PCQAP/MED15 has connections to cancer and certain neurological disorders. Its misregulation or mutation can lead to transcriptional dysregulation contributing to the onset of cancers by affecting genes that control cell proliferation. In neurological disorders PCQAP/MED15 impacts the expression of genes involved in neural functions with possible ties to disorders like Huntington's disease. The protein interacts with p53 a well-known tumor suppressor protein highlighting its role in disease development and progression.
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Specifications

Form

Liquid

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General info

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway.

Sequence similarities

Belongs to the Mediator complex subunit 15 family.

Post-translational modifications

Ubiquitinated by TRIM11, leading to proteasomal degradation.

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Product protocols

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Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway.
See full target information MED15
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Product promise

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