Recombinant human PCSK9 protein (Active) (Biotin) is a Human Full Length protein, in the 31 to 692 aa range, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Conjugation
- Biotin
Amino acid sequence
Q E D E D G D Y E E L V L A L R S E E D G L A E A P E H G T T A T F H R C A K D P W R L P G T Y V V V L K E E T H L S Q S E R T A R R L Q A Q A A R R G Y L T K I L H V F H G L L P G F L V K M S G D L L E L A L K L P H V D Y I E E D S S V F A Q S I P W N L E R I T P P R Y R A D E Y Q P P D G G S L V E V Y L L D T S I Q S D H R E I E G R V M V T D F E N V P E E D G T R F H R Q A S K C D S H G T H L A G V V S G R D A G V A K G A S M R S L R V L N C Q G K G T V S G T L I G L E F I R K S Q L V Q P V G P L V V L L P L A G G Y S R V L N A A C Q R L A R A G V V L V T A A G N F R D D A C L Y S P A S A P E V I T V G A T N A Q D Q P V T L G T L G T N F G R C V D L F A P G E D I I G A S S D C S T C F V S Q S G T S Q A A A H V A G I A A M M L S A E P E L T L A E L R Q R L I H F S A K D V I N E A W F P E D Q R V L T P N L V A A L P P S T H G A G W Q L F C R T V W S A H S G P T R M A T A V A R C A P D E E L L S C S S F S R S G K R R G E R M E A Q G G K L V C R A H N A F G G E G V Y A I A R C C L L P Q A N C S V H T A P P A E A S M G T R V H C H Q Q G H V L T G C S S H W E V E D L G T H K P P V L R P R G Q P N Q C V G H R E A S I H A S C C H A P G L E C K V K E H G I P A P Q E Q V T V A C E E G W T L T G C S A L P G T S H V L G A Y A V D N T C V V R S R D V S T T G S T S E G A V T A V A I C C R S R H L A Q A S Q E L Q G G G L N D I F E A Q K I E W H E H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments (PubMed:18039658). Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation (PubMed:17461796, PubMed:18197702, PubMed:18799458, PubMed:22074827). Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway (PubMed:18660751). Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
Alternative names
NARC1, PSEC0052, PCSK9, Proprotein convertase subtilisin/kexin type 9, Neural apoptosis-regulated convertase 1, Proprotein convertase 9, Subtilisin/kexin-like protease PC9, NARC-1, PC9
Recommended products
Recombinant human PCSK9 protein (Active) (Biotin) is a Human Full Length protein, in the 31 to 692 aa range, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- 50 μL reaction mix (50 mM HEPES pH 7.4, 50 mM NaCl, 200 μM CaCl2, 0.05% Tween-20, 0.1% BSA, and 0–1500 ng biotin-labeled PCSK9) is added to wells precoated with LDLR Ectodomain. Incubate for 2 hr. Add streptavidin-labeled HRP and incubate 1 hour. Finally, add HRP chemiluminescent substrates and read luminescence.
- Conjugation
- Biotin
- Accession
- Q8NBP7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.48% Tris, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- Q E D E D G D Y E E L V L A L R S E E D G L A E A P E H G T T A T F H R C A K D P W R L P G T Y V V V L K E E T H L S Q S E R T A R R L Q A Q A A R R G Y L T K I L H V F H G L L P G F L V K M S G D L L E L A L K L P H V D Y I E E D S S V F A Q S I P W N L E R I T P P R Y R A D E Y Q P P D G G S L V E V Y L L D T S I Q S D H R E I E G R V M V T D F E N V P E E D G T R F H R Q A S K C D S H G T H L A G V V S G R D A G V A K G A S M R S L R V L N C Q G K G T V S G T L I G L E F I R K S Q L V Q P V G P L V V L L P L A G G Y S R V L N A A C Q R L A R A G V V L V T A A G N F R D D A C L Y S P A S A P E V I T V G A T N A Q D Q P V T L G T L G T N F G R C V D L F A P G E D I I G A S S D C S T C F V S Q S G T S Q A A A H V A G I A A M M L S A E P E L T L A E L R Q R L I H F S A K D V I N E A W F P E D Q R V L T P N L V A A L P P S T H G A G W Q L F C R T V W S A H S G P T R M A T A V A R C A P D E E L L S C S S F S R S G K R R G E R M E A Q G G K L V C R A H N A F G G E G V Y A I A R C C L L P Q A N C S V H T A P P A E A S M G T R V H C H Q Q G H V L T G C S S H W E V E D L G T H K P P V L R P R G Q P N Q C V G H R E A S I H A S C C H A P G L E C K V K E H G I P A P Q E Q V T V A C E E G W T L T G C S A L P G T S H V L G A Y A V D N T C V V R S R D V S T T G S T S E G A V T A V A I C C R S R H L A Q A S Q E L Q G G G L N D I F E A Q K I E W H E H H H H H H
- Accession
- Q8NBP7
- Protein length
- Full Length
- Predicted molecular weight
- 73.8 kDa
- Amino acids
- 31 to 692
- Nature
- Recombinant
- Tags
- Avi tag C-Terminus, His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments (PubMed:18039658). Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation (PubMed:17461796, PubMed:18197702, PubMed:18799458, PubMed:22074827). Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway (PubMed:18660751). Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
- Sequence similarities
Belongs to the peptidase S8 family.
- Post-translational modifications
Cleavage by furin and PCSK5 generates a truncated inactive protein that is unable to induce LDLR degradation.
- Subcellular localisation
- Endosome, Lysosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Enzymatically biotin-labeled using Avi-tag™ technology
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a protein responsible for the regulation of cholesterol levels by binding to low-density lipoprotein receptors (LDLR) on hepatocytes. This interaction marks the LDLRs for degradation reducing the liver's ability to clear LDL cholesterol from the blood. PCSK9 has a molecular weight of approximately 74 kDa. The protein is mainly expressed in the liver but is also found in the intestine and kidneys. PCSK9 is referred to as NARC-1 standing for neural apoptosis-regulated convertase 1 highlighting its role in the liver's cholesterol management system.
- Biological function summary
PCSK9 influences cholesterol homeostasis by its important role in degrading LDL receptors. It functions independently rather than as part of larger protein complexes. PCSK9 gains particular interest in therapeutic contexts where its inhibition can lead to increased LDLR levels and enhanced clearance of LDL cholesterol. Biotinylated PCSK9 and mouse PCSK9 variants provide significant tools for experimental study. Kits such as the PCSK9 ELISA kit enable detailed measurement of PCSK9 levels in blood samples providing insights into cholesterol metabolism dynamics.
- Pathways
PCSK9 operates within the lipid metabolism pathway and the cholesterol biosynthesis pathway. The protein's activity affects the fate of LDL cholesterol within these pathways. It interacts with proteins such as apolipoprotein B (ApoB) which plays a central role in the structural component of LDL particles. The modulation of these pathways by PCSK9 highlights the significance of its function in maintaining cardiovascular health and managing cholesterol levels.
- Associated diseases and disorders
PCSK9 is closely linked to hypercholesterolemia and coronary artery disease. Mutations in PCSK9 can lead to autosomal dominant hypercholesterolemia due to its effect on LDL receptor degradation. Other proteins such as ApoB and LDLR are involved in these conditions tightly interacting with PCSK9's regulatory function. A better understanding of PCSK9's role offers potential therapeutic targets for cardiovascular disease interventions especially through the development of PCSK9 antibodies and PCSK9 assays that adjust cholesterol levels.
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2 product images
Functional Studies - Recombinant human PCSK9 protein (Active) (Biotin) (ab271653)
Specific activity of ab271653.
SDS-PAGE - Recombinant human PCSK9 protein (Active) (Biotin) (ab271653)
SDS-PAGE analysis of 4 μg ab271653.
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