Recombinant human PCTAIRE1 protein (Active) is a Human Fragment protein, in the 107 to 496 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Amino acid sequence
R K I S T E D I N K R L S L P A D I R L P E G Y L E K L T L N S P I F D K P L S R R L R R V S L S E I G F G K L E T Y I K L D K L G E G T Y A T V Y K G K S K L T D N L V A L K E I R L E H E E G A P C T A I R E V S L L K D L K H A N I V T L H D I I H T E K S L T L V F E Y L D K D L K Q Y L D D C G N I I N M H N V K L F L F Q L L R G L A Y C H R Q K V L H R D L K P Q N L L I N E R G E L K L A D F G L A R A K S I P T K T Y S N E V V T L W Y R P P D I L L G S T D Y S T Q I D M W G V G C I F Y E M A T G R P L F P G S T V E E Q L H F I F R I L G T P T E E T W P G I L S N E E F K T Y N Y P K Y R A E A L L S H A P R L D S D G A D L L T K L L Q F E G R N R I S A E D A M K H P F F L S L G E R I H K L P D T T S I F A L K E I Q L Q K E A S L R S S S M P D S G R P A F R V V D T E F G N T T S C C V S S S P K L R R N A H S R L E S Y R P D T D L S R E D T G C N L Q H I S D R E N I D D L N M E F N P S D H P R A S T I F L S K S Q T D V R E K R K S L F I N H H P P G Q I A R K Y S S C S T I F L D D S T V S Q P N L K Y T I K C V A L A I Y Y H I K N R D P D G R M L L D I F D E N L H P L S K S E V P P D Y D K H N P E Q K Q I Y R F V R T L F S A A Q L T A E C A I V T L V Y L E R L L T Y A E I D I C P A N W K R I V L G A I L L A S K V W D D Q A V W N V D Y C Q I L K D I T V E D M N E L E R Q F L E L L Q F N I N V P S S V Y A K Y Y F D L R S L A E A N N L S F P L E P L S R E R A H K L E A I S R L C E D K Y K D L R R S A R K R S A S A D N L T L P R W S P A I I S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro).
Alternative names
PCTAIRE1, PCTK1, CDK16, Cyclin-dependent kinase 16, Cell division protein kinase 16, PCTAIRE-motif protein kinase 1, Serine/threonine-protein kinase PCTAIRE-1
Recommended products
Recombinant human PCTAIRE1 protein (Active) is a Human Fragment protein, in the 107 to 496 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab177586 was determined to be 40 nmol/mim/mg.
- Accession
- Q00536-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris-HCl buffer, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- R K I S T E D I N K R L S L P A D I R L P E G Y L E K L T L N S P I F D K P L S R R L R R V S L S E I G F G K L E T Y I K L D K L G E G T Y A T V Y K G K S K L T D N L V A L K E I R L E H E E G A P C T A I R E V S L L K D L K H A N I V T L H D I I H T E K S L T L V F E Y L D K D L K Q Y L D D C G N I I N M H N V K L F L F Q L L R G L A Y C H R Q K V L H R D L K P Q N L L I N E R G E L K L A D F G L A R A K S I P T K T Y S N E V V T L W Y R P P D I L L G S T D Y S T Q I D M W G V G C I F Y E M A T G R P L F P G S T V E E Q L H F I F R I L G T P T E E T W P G I L S N E E F K T Y N Y P K Y R A E A L L S H A P R L D S D G A D L L T K L L Q F E G R N R I S A E D A M K H P F F L S L G E R I H K L P D T T S I F A L K E I Q L Q K E A S L R S S S M P D S G R P A F R V V D T E F G N T T S C C V S S S P K L R R N A H S R L E S Y R P D T D L S R E D T G C N L Q H I S D R E N I D D L N M E F N P S D H P R A S T I F L S K S Q T D V R E K R K S L F I N H H P P G Q I A R K Y S S C S T I F L D D S T V S Q P N L K Y T I K C V A L A I Y Y H I K N R D P D G R M L L D I F D E N L H P L S K S E V P P D Y D K H N P E Q K Q I Y R F V R T L F S A A Q L T A E C A I V T L V Y L E R L L T Y A E I D I C P A N W K R I V L G A I L L A S K V W D D Q A V W N V D Y C Q I L K D I T V E D M N E L E R Q F L E L L Q F N I N V P S S V Y A K Y Y F D L R S L A E A N N L S F P L E P L S R E R A H K L E A I S R L C E D K Y K D L R R S A R K R S A S A D N L T L P R W S P A I I S
- Accession
- Q00536
- Protein length
- Fragment
- Predicted molecular weight
- 66 kDa
- Amino acids
- 107 to 496
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purity assessed by densitometry. Affinity purified.
General info
- Function
Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro).
- Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
- Post-translational modifications
Phosphorylation of CDK16 is essential for the binding of CCNY, but also essential for the regulation of CDK16 kinase activity (PubMed:22798068). Phosphorylation of CDK16 is essential for the binding of CCNYl1, but also essential for the regulation of CDK16 kinase activity (By similarity). Ser-146 and Ser-153 are the most critical sites for the binding of CCNYL1 and for modulating CDK16 kinase activity (By similarity). Phosphorylation at Ser-153 inhibits kinase activity (PubMed:22798068).
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Recombinant Human Rb protein ab56270 (Human Rb protein fragment), ab89813 (Histone H1 protein) and Native Pig Myelin Basic Protein ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PCTAIRE1 also known as Cyclin-dependent kinase 16 (CDK16) plays a significant mechanical role as a serine/threonine kinase. This protein with a molecular weight of approximately 45 kDa participates in various intracellular processes. It shows expression in multiple tissues including prominently in the brain and testis. PCTAIRE1 regulates cellular events by phosphorylating target proteins thereby influencing their function.
- Biological function summary
PCTAIRE1 influences certain cellular functions such as signal transduction and neuron differentiation. It exists as part of complexes that include cyclins which modulate its kinase activity. In neurons PCTAIRE1 contributes to processes like neurite outgrowth and synaptic function. Research indicates a role in the transport of synaptic vesicles hinting at its involvement in neurotransmitter release and communication between neurons.
- Pathways
PCTAIRE1 serves as a component of pathways such as the Wnt signaling pathway and the cell cycle. Its function aligns with several other proteins including CDK16 in modulating processes like cell proliferation and neural development. PCTAIRE1 interacts closely with proteins like Cyclin Y which help guide its role in these pathways ensuring proper progression of related biological events.
- Associated diseases and disorders
PCTAIRE1 has associations with neurological disorders and certain cancers. Abnormal expression or activity of PCTAIRE1 may relate to conditions like schizophrenia or glioblastoma. The protein's interaction with Cyclin Y is key in the context of cancer where dysregulation of these interactions can contribute to tumor progression. Alterations in PCTAIRE1 function and its pathways highlight its potential as a target for therapeutic strategies in these diseases.
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2 product images
SDS-PAGE - Recombinant human PCTAIRE1 protein (Active) (ab177586)
The purity of ab177586 was determined to be >90% by densitometry.
Functional Studies - Recombinant human PCTAIRE1 protein (Active) (ab177586)
The specific activity of ab177586 was determined to be 40 nmol/mim/mg.
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