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AB177586

Recombinant human PCTAIRE1 protein (Active)

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(1 Publication)

Recombinant human PCTAIRE1 protein (Active) is a Human Fragment protein, in the 107 to 496 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

PCTAIRE1, PCTK1, CDK16, Cyclin-dependent kinase 16, Cell division protein kinase 16, PCTAIRE-motif protein kinase 1, Serine/threonine-protein kinase PCTAIRE-1

2 Images
Functional Studies - Recombinant human PCTAIRE1 protein (Active) (AB177586)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PCTAIRE1 protein (Active) (AB177586)

The specific activity of ab177586 was determined to be 40 nmol/mim/mg.

SDS-PAGE - Recombinant human PCTAIRE1 protein (Active) (AB177586)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PCTAIRE1 protein (Active) (AB177586)

The purity of ab177586 was determined to be >90% by densitometry.

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, WB, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab177586 was determined to be 40 nmol/mim/mg.

Accession

Q00536

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris-HCl buffer, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Complementary Products

Primary Antibodies

AB181208

Anti-PCTAIRE1 antibody [EPR13210]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-PCTAIRE1 antibody [EPR13210] (AB181208)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Biochemicals

AB147242

MK-1775

Chemical Structure - MK-1775 (AB147242)

  • 0
  • 0
Proteins & Peptides

AB107698

Recombinant human VRK2 protein

Functional Studies - Recombinant human VRK2 protein (AB107698)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab56270 (Human Rb protein fragment), ab89813 (Histone H1 protein) and ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"sequence":"RKISTEDINKRLSLPADIRLPEGYLEKLTLNSPIFDKPLSRRLRRVSLSEIGFGKLETYIKLDKLGEGTYATVYKGKSKLTDNLVALKEIRLEHEEGAPCTAIREVSLLKDLKHANIVTLHDIIHTEKSLTLVFEYLDKDLKQYLDDCGNIINMHNVKLFLFQLLRGLAYCHRQKVLHRDLKPQNLLINERGELKLADFGLARAKSIPTKTYSNEVVTLWYRPPDILLGSTDYSTQIDMWGVGCIFYEMATGRPLFPGSTVEEQLHFIFRILGTPTEETWPGILSNEEFKTYNYPKYRAEALLSHAPRLDSDGADLLTKLLQFEGRNRISAEDAMKHPFFLSLGERIHKLPDTTSIFALKEIQLQKEASLRSSSMPDSGRPAFRVVDTEFGNTTSCCVSSSPKLRRNAHSRLESYRPDTDLSREDTGCNLQHISDRENIDDLNMEFNPSDHPRASTIFLSKSQTDVREKRKSLFINHHPPGQIARKYSSCSTIFLDDSTVSQPNLKYTIKCVALAIYYHIKNRDPDGRMLLDIFDENLHPLSKSEVPPDYDKHNPEQKQIYRFVRTLFSAAQLTAECAIVTLVYLERLLTYAEIDICPANWKRIVLGAILLASKVWDDQAVWNVDYCQILKDITVEDMNELERQFLELLQFNINVPSSVYAKYYFDLRSLAEANNLSFPLEPLSRERAHKLEAISRLCEDKYKDLRRSARKRSASADNLTLPRWSPAIIS","proteinLength":"Fragment","predictedMolecularWeight":"66 kDa","actualMolecularWeight":null,"aminoAcidEnd":496,"aminoAcidStart":107,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q00536","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCTAIRE1 also known as Cyclin-dependent kinase 16 (CDK16) plays a significant mechanical role as a serine/threonine kinase. This protein with a molecular weight of approximately 45 kDa participates in various intracellular processes. It shows expression in multiple tissues including prominently in the brain and testis. PCTAIRE1 regulates cellular events by phosphorylating target proteins thereby influencing their function.
Biological function summary

PCTAIRE1 influences certain cellular functions such as signal transduction and neuron differentiation. It exists as part of complexes that include cyclins which modulate its kinase activity. In neurons PCTAIRE1 contributes to processes like neurite outgrowth and synaptic function. Research indicates a role in the transport of synaptic vesicles hinting at its involvement in neurotransmitter release and communication between neurons.

Pathways

PCTAIRE1 serves as a component of pathways such as the Wnt signaling pathway and the cell cycle. Its function aligns with several other proteins including CDK16 in modulating processes like cell proliferation and neural development. PCTAIRE1 interacts closely with proteins like Cyclin Y which help guide its role in these pathways ensuring proper progression of related biological events.

PCTAIRE1 has associations with neurological disorders and certain cancers. Abnormal expression or activity of PCTAIRE1 may relate to conditions like schizophrenia or glioblastoma. The protein's interaction with Cyclin Y is key in the context of cancer where dysregulation of these interactions can contribute to tumor progression. Alterations in PCTAIRE1 function and its pathways highlight its potential as a target for therapeutic strategies in these diseases.
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Specifications

Form

Liquid

Additional notes

Purity assessed by densitometry. Affinity purified.

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General info

Function

Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro).

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Post-translational modifications

Phosphorylation of CDK16 is essential for the binding of CCNY, but also essential for the regulation of CDK16 kinase activity (PubMed:22798068). Phosphorylation of CDK16 is essential for the binding of CCNYl1, but also essential for the regulation of CDK16 kinase activity (By similarity). Ser-146 and Ser-153 are the most critical sites for the binding of CCNYL1 and for modulating CDK16 kinase activity (By similarity). Phosphorylation at Ser-153 inhibits kinase activity (PubMed:22798068).

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Product protocols

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Target data

Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro).
See full target information CDK16
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10028 PubMed39562571

2024

A Huluwa phosphorylation switch regulates embryonic axis induction.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Li,Yun Yan,Bo Gong,Qianwen Zheng,Haiyan Zhou,Jiarui Sun,Mingpeng Li,Zhao Wang,Yaohui Li,Yunjing Wan,Weixi Chen,Shiqian Qi,Xianming Mo,Anming Meng,Bo Xiang,Jing Chen
View all publications
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Product promise

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