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AB123157

Recombinant Human PCYT2 protein (His tag N-Terminus)

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Recombinant Human PCYT2 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 389 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Ethanolamine-phosphate cytidylyltransferase, CTP:phosphoethanolamine cytidylyltransferase, Phosphorylethanolamine transferase, PCYT2

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SDS-PAGE - Recombinant Human PCYT2 protein (His tag N-Terminus) (AB123157)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PCYT2 protein (His tag N-Terminus) (AB123157)

15% SDS-PAGE analysis of 3 µg of Human PCYT2 protein (ab123157).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q99447

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMIRNGRGAAGGAEQPGPGGRRAVRVWCDGCYDMVHYGHSNQLRQARAMGDYLIVGVHTDEEIAKHKGPPVFTQEERYKMVQAIKWVDEVVPAAPYVTTLETLDKYNCDFCVHGNDITLTVDGRDTYEEVKQAGRYRECKRTQGVSTTDLVGRMLLVTKAHHSSQEMSSEYREYADSFGKCPGGRNPWTGVSQFLQTSQKIIQFASGKEPQPGETVIYVAGAFDLFHIGHVDFLEKVHRLAERPYIIAGLHFDQEVNHYKGKNYPIMNLHERTLSVLACRYVSEVVIGAPYAVTAELLSHFKVDLVCHGKTEIIPDRDGSDPYQEPKRRGIFRQIDSGSNLTTDLIVQRIITNRLEYEARNQKKEAKELAFLEAARQQAAQPLGERDGDF","proteinLength":"Full Length","predictedMolecularWeight":"45.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":389,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q99447","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCYT2 also known as ethanolamine-phosphate cytidylyltransferase is an enzyme that plays a critical role in phospholipid metabolism. It has a molecular mass of approximately 41 kDa. PCYT2 is expressed in various tissues with high levels found in the liver and brain. The enzyme catalyzes the conversion of phosphoethanolamine and CTP to CDP-ethanolamine an important step in the CDP-ethanolamine pathway which synthesizes phosphatidylethanolamine.
Biological function summary

The function of PCYT2 centers around lipid metabolism and membrane biosynthesis. PCYT2 acts as an integral component of the CDP-ethanolamine pathway which is essential for the production of phosphatidylethanolamine a principal phospholipid in cell membranes. It works in conjunction with other enzymes to ensure the proper biosynthesis of phospholipids. Although its precise participation as part of a larger protein complex remains uncertain its enzymatic activity is significant for lipid homeostasis.

Pathways

The activity of PCYT2 mainly fits into the phosphatidylserine decarboxylation and CDP-ethanolamine pathways. The enzyme interacts with CDP-diacylglycerol and phosphatidylcholine playing a significant role in lipid assembly and remodeling of cellular membranes. It shares functional interactions within these pathways with other enzymes like CHPT1 which further converges toward the regulation of phosphatidylcholine and phosphatidylethanolamine synthesis.

Mutations in PCYT2 have been linked to neurodegenerative diseases and certain metabolic disorders. Its dysfunction can contribute to neurological symptoms due to its fundamental role in maintaining normal brain phospholipid levels. The protein's connections to disorders particularly concerning phospholipid imbalance include interactions with related enzymes like EPT1. Such connections underline how PCYT2 disruptions can impact phospholipid biosynthesis and potential disease outcomes.
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Specifications

Form

Liquid

Additional notes

ab123157 was purified using conventional chromatography.

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General info

Function

Ethanolamine-phosphate cytidylyltransferase that catalyzes the second step in the synthesis of phosphatidylethanolamine (PE) from ethanolamine via the CDP-ethanolamine pathway (PubMed : 31637422, PubMed : 9083101). Phosphatidylethanolamine is a dominant inner-leaflet phospholipid in cell membranes, where it plays a role in membrane function by structurally stabilizing membrane-anchored proteins, and participates in important cellular processes such as cell division, cell fusion, blood coagulation, and apoptosis (PubMed : 9083101).

Sequence similarities

Belongs to the cytidylyltransferase family.

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Product protocols

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Target data

Ethanolamine-phosphate cytidylyltransferase that catalyzes the second step in the synthesis of phosphatidylethanolamine (PE) from ethanolamine via the CDP-ethanolamine pathway (PubMed : 31637422, PubMed : 9083101). Phosphatidylethanolamine is a dominant inner-leaflet phospholipid in cell membranes, where it plays a role in membrane function by structurally stabilizing membrane-anchored proteins, and participates in important cellular processes such as cell division, cell fusion, blood coagulation, and apoptosis (PubMed : 9083101).
See full target information PCYT2
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